Recombinant Anti-CBR3 antibody [EPR14129] (ab180939)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14129] to CBR3
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CBR3 antibody [EPR14129]
See all CBR3 primary antibodies -
Description
Rabbit monoclonal [EPR14129] to CBR3 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- HeLa, K562 and 293 cell lysate. K562 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
-
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14129 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180939 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt (Intra) |
1/130.
|
|
WB | (1) |
1/10000 - 1/50000. Predicted molecular weight: 30 kDa.
|
Notes |
---|
Flow Cyt (Intra)
1/130. |
WB
1/10000 - 1/50000. Predicted molecular weight: 30 kDa. |
Target
-
Function
Has low NADPH-dependent oxidoreductase activity towards 4-benzoylpyridine and menadione (in vitro). -
Tissue specificity
Detected in ovary, pancreas, intestine, colon, kidney, brain, thymus, lung, heart, liver, spleen, leukocyte, prostate and testis. -
Sequence similarities
Belongs to the short-chain dehydrogenases/reductases (SDR) family. -
Cellular localization
Cytoplasm. - Information by UniProt
-
Database links
- Entrez Gene: 874 Human
- Entrez Gene: 109857 Mouse
- Entrez Gene: 304078 Rat
- Omim: 603608 Human
- SwissProt: O75828 Human
- SwissProt: Q8K354 Mouse
- Unigene: 154510 Human
- Unigene: 4512 Mouse
-
Alternative names
- Carbonyl reductase (NADPH) 3 antibody
- Carbonyl reductase (NADPH) 3 EC 1.1.1.184 antibody
- Carbonyl reductase [NADPH] 3 antibody
see all
Images
-
All lanes : Anti-CBR3 antibody [EPR14129] (ab180939) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : 293 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 30 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
-
Intracellular Flow Cytometry analysis of K562 cells labeling CBR3 using ab180939 at a 1/130 dilution (pink). Goat anti rabbit IgG (FITC) used as the secondary at a 1/150 dilution. Isotype control Rabbit monoclonal IgG (green). Cells were fixed in 2% paraformaldehyde.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab180939 has not yet been referenced specifically in any publications.