Recombinant Anti-CCL23 antibody [EPR11363] (ab171751)
![Western blot - Anti-CCL23 antibody [EPR11363] (ab171751)](https://www.abcam.com/ps/products/171/ab171751/Images/ab171751-172220-anti-ccl23-antibody-epr11363-western-blot.jpg "Western blot - Anti-CCL23 antibody [EPR11363] (ab171751)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11363] to CCL23
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CCL23 antibody [EPR11363]
See all CCL23 primary antibodies -
Description
Rabbit monoclonal [EPR11363] to CCL23 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CCL23 aa 50 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P55773 -
Positive control
- Human CCL23 recombinant protein; Human liver and pancreas tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR11363 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab171751 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 13 kDa.
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/1000 - 1/5000. Predicted molecular weight: 13 kDa. |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
Tissue specificity
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
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Alternative names
- C C motif chemokine ligand 23 antibody
- C-C motif chemokine 23 antibody
- C6 beta chemokine antibody
see all
Images
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Western blot - Anti-CCL23 antibody [EPR11363] (ab171751)Anti-CCL23 antibody [EPR11363] (ab171751) at 1/1000 dilution + Human recombinant CCL23 at 0.005 µg
Predicted band size: 13 kDaNote: Primary antibody diluted in 1% BSA.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] (ab171751)](https://www.abcam.com/ps/products/171/ab171751/Images/ab171751-172219-anti-ccl23-antibody-epr11363-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] (ab171751)Immunohistochemistry of paraffin-embedded Human pancreas tissue labeling CCL23 using ab171751 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] (ab171751)](https://www.abcam.com/ps/products/171/ab171751/Images/ab171751-172218-anti-ccl23-antibody-epr11363-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL23 antibody [EPR11363] (ab171751)Immunohistochemistry of paraffin-embedded Human liver tissue labeling CCL23 using ab171751 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-CCL23 antibody [EPR11363] (ab171751)](https://www.abcam.com/ps/products/171/ab171751/Images/ab171751-4-benefits-of-recombinant-antibodies.png)
Anti-CCL23 antibody [EPR11363] (ab171751)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab171751 has not yet been referenced specifically in any publications.