Recombinant Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)
![Western blot - Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)](https://www.abcam.com/ps/products/249/ab249623/Images/ab249623-402860-anti-ccl23-antibody-epr11364-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11364] to CCL23 - BSA and Azide free
- Suitable for: ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CCL23 antibody [EPR11364] - BSA and Azide free
See all CCL23 primary antibodies -
Description
Rabbit monoclonal [EPR11364] to CCL23 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CCL23 aa 1-100. The exact sequence is proprietary.
Database link: P55773 -
General notes
ab249623 is the carrier-free version of ab170958.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EPR11364 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab249623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa.
|
| Notes |
|---|
|
ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 13 kDa. |
Target
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Function
Shows chemotactic activity for monocytes, resting T-lymphocytes, and neutrophils, but not for activated lymphocytes. Inhibits proliferation of myeloid progenitor cells in colony formation assays. This protein can bind heparin. Binds CCR1. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are more potent chemoattractants than the small-inducible cytokine A23. -
Tissue specificity
High levels in adult lung, liver, skeletal muscle and pancreas. Moderate levels in fetal liver, adult bone marrow and placenta. The short form is the major species and the longer form was detected only in very low abundance. CCL23(19-99), CCL23(22-99), CCL23(27-99), CCL23(30-99) are found in high levels in synovial fluids from rheumatoid patients. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 6368 Human
- Omim: 602494 Human
- SwissProt: P55773 Human
- Unigene: 169191 Human
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Alternative names
- C C motif chemokine ligand 23 antibody
- C-C motif chemokine 23 antibody
- C6 beta chemokine antibody
see all
Images
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Western blot - Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)Anti-CCL23 antibody [EPR11364] (ab170958) at 1/1000 dilution + Human CCL23 recombinant protein (5ng)
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 13 kDaThis data was developed using ab170958, the same antibody clone in a different buffer formulation.
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![Immunocytochemistry/ Immunofluorescence - Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)](https://www.abcam.com/ps/products/249/ab249623/Images/ab249623-1-anti-ccl23-antibody-epr11364-bsa-and-azide-free-immunocytochemistry.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623) -
![Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)](https://www.abcam.com/ps/products/249/ab249623/Images/ab249623-3-benefits-of-recombinant-antibodies.png)
Anti-CCL23 antibody [EPR11364] - BSA and Azide free (ab249623)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab249623 has not yet been referenced specifically in any publications.
