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RecombinantRabMAb

Recombinant Anti-CCN3 antibody [EPR8781(3)] (ab191425)

Submit a review Submit a question References (2)
Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
  • Anti-CCN3 antibody [EPR8781(3)] (ab191425)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8781(3)] to CCN3
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CCN3 antibody [EPR8781(3)]
    See all CCN3 primary antibodies

  • Description

    Rabbit monoclonal [EPR8781(3)] to CCN3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa, 293, Caco-2 and TT lysates; Human hepatocellular carcinoma and Human pancreas tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR8781(3)

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab191425 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 39 kDa).
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Immediate-early protein likely to play a role in cell growth regulation.

  • Tissue specificity

    Expressed in bone marrow, thymic cells and nephroblastoma. Increased expression in Wilms tumor of the stromal type.

  • Sequence similarities

    Belongs to the CCN family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
    Contains 1 IGFBP N-terminal domain.
    Contains 1 TSP type-1 domain.
    Contains 1 VWFC domain.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • CCN family member 3 antibody
    • CCN3 antibody
    • IBP-9 antibody
    • IGF-binding protein 9 antibody
    • IGFBP-9 antibody
    • IGFBP9 antibody
    • Insulin-like growth factor-binding protein 9 antibody
    • Nephroblastoma overexpressed antibody
    • Nephroblastoma overexpressed gene protein homolog antibody
    • Nephroblastoma-overexpressed gene protein homolog antibody
    • NOV antibody
    • NOV_HUMAN antibody
    • NovH antibody
    • Protein NOV homolog antibody
    see all

Images

  • Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    All lanes : Anti-CCN3 antibody [EPR8781(3)] (ab191425) at 1/10000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : Caco-2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CCN3 with ab191425 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Western blot - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Anti-CCN3 antibody [EPR8781(3)] (ab191425) at 1/5000 dilution + TT lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCN3 antibody [EPR8781(3)] (ab191425)

    Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling CCN3 with ab191425 at 1/100 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.  Inset: Negative control: using PBS instead of primary antibody.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-CCN3 antibody [EPR8781(3)] (ab191425)
    Anti-CCN3 antibody [EPR8781(3)] (ab191425)

References (2)

Publishing research using ab191425? Please let us know so that we can cite the reference in this datasheet.

ab191425 has been referenced in 2 publications.

  • Chen R  et al. SNAIL regulates gastric carcinogenesis through CCN3 and NEFL. Carcinogenesis 42:190-201 (2021). PubMed: 33313663
  • Zhu HP  et al. Regulatory mechanism of NOV/CCN3 in the inflammation and apoptosis of lung epithelial alveolar cells upon lipopolysaccharide stimulation. Mol Med Rep 21:1872-1880 (2020). PubMed: 31545412

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