Name: Anti-CD10 antibody [EPR22867-118]
Brand: RabMAb
SKU: ab256494
Rating: 5 (5 reviews)

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Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22867-118] to CD10
Suitable for: ICC/IF, WB, IHC-P, IP, IHC-Fr
Knockout validated
Reacts with: Mouse, Rat, Human

Carrier Free

Product name

Anti-CD10 antibody [EPR22867-118]
See all CD10 primary antibodies
Description

Rabbit monoclonal [EPR22867-118] to CD10
Host species

Rabbit
Tested applications

Suitable for: ICC/IF, WB, IHC-P, IP, IHC-Frmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild type HAP1, Raji and Ramos whole cell lysate, Rat lung, Rat kidney, Human tonsil and Mouse lung lysates. IHC-P: Human kidney, Human placenta, Mouse kidney and Rat kidney tissues. IHC-Fr: Mouse and rat kidney tissue. ICC/IF: Ramos, WEHI-231 and 2.4G2 cells. IP: Raji cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 0.5% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22867-118
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD10 antibody [EPR22867-118] - BSA and Azide free (ab261729)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CD10 protein (ab155720)
Related Products

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab256494 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
ICC/IF	(1)	1/500.
WB	(1)	1/1000. Predicted molecular weight: 85 kDa.
IHC-P	(1)	1/500.
IP		1/30.
IHC-Fr	(1)	1/100.

Notes
ICC/IF 1/500.
WB 1/1000. Predicted molecular weight: 85 kDa.
IHC-P 1/500.
IP 1/30.
IHC-Fr 1/100.

Application notes

Is unsuitable for Flow Cyt.

Function

Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
Sequence similarities

Belongs to the peptidase M13 family.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P08473 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Entrez Gene: 24590 Rat
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- SwissProt: P07861 Rat
- Unigene: 307734 Human
- Unigene: 296022 Mouse
- Unigene: 33598 Rat
see all
Alternative names
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
- CD10 antigen antibody
- Common acute lymphocytic leukemia antigen antibody
- DKFZp686O16152 antibody
- EC 3.4.24.11 antibody
- Enkephalinase antibody
- EPN antibody
- Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase) antibody
- Membrane metallo endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10) antibody
- Membrane metallo endopeptidase antibody
- Membrane metallo endopeptidase variant 1 antibody
- Membrane metallo endopeptidase variant 2 antibody
- Membrane metalloendopeptidase antibody
- Membrane metalloendopeptidase neutral endopeptidase enkephalinase antibody
- Membrane metalloendopeptidase neutral endopeptidase enkephalinase CALLA CD10 antibody
- Membrane metalloendopeptidase variant 1 antibody
- Membrane metalloendopeptidase variant 2 antibody
- MGC126681 antibody
- MGC126707 antibody
- MME antibody
- NEP antibody
- NEP_HUMAN antibody
- Neprilysin antibody
- neprilysin-390 antibody
- neprilysin-411 antibody
- Neutral endopeptidase 24.11 antibody
- Neutral endopeptidase antibody
- Neutral endopeptidase, membrane-associated antibody
- SFE antibody
- Skin fibroblast elastase antibody
see all

Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494)

All lanes : Anti-CD10 antibody [EPR22867-118] (ab256494) at 1/1000 dilution

Lane 1 : Rat lung tissue lysate at 10 µg
Lane 2 : Rat kidney tissue lysate at 10 µg
Lane 3 : Human tonsil tissue lysate at 10 µg
Lane 4 : Mouse lung tissue lysate at 20 µg

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

The molecular weight observed is consistent with what has been described in the literature (PMID:15286660)

Exposure time: Lane 1/3: 8 seconds Lane 2: 1 second Lane 4: 3 seconds
Western blot - Anti-CD10 antibody [EPR22867-118] (ab256494)

All lanes : Anti-CD10 antibody [EPR22867-118] (ab256494) at 1/1000 dilution

Lane 1 : Wild type HAP1 whole cell lysate
Lane 2 : CD10 knockout HAP1 whole cell lysate
Lane 3 : Raji (human Burkitts lymphoma B lymphocyte), whole cell lysate
Lane 4 : Ramos (human Burkitts lymphoma B lymphocyte), whole cell lysate
Lane 5 : HT-29 (human colorectal adenocarcinoma epithelial cell), whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST

ab256494 was shown to specifically react with CD10 in wild-type HAP1 cells as signal was lost in CD10 knockout cells. Wild-type and CD10 knockout samples were subjected to SDS-PAGE. ab256494 and ab181602 (Rabbit anti-GAPDH loading control) were incubated 1 hour at room temperature at 1/1000 dilution and 1/200,000 dilution respectively. Blots were developed with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) secondary antibody at 1/100,000 dilution for 1 hour at room temperature before imaging.

The molecular weight observed is consistent with what has been described in the literature (PMID:15286660) Negative control: HT-29 (PMID:19828468).

Exposure time: 59 seconds
Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CD10 with ab256494 at 1/100 (5.45 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution.
Immunohistochemistry (Frozen sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunohistochemical analysis of 4% PFA fixed 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling CD10 with ab256494 at 1:100 (5.45 µg/ml) dilution followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 (2 µg/ml) dilution. The nuclear counterstain was DAPI (Blue). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody was ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1:1000 (2 µg/ml) dilution.
Immunoprecipitation - Anti-CD10 antibody [EPR22867-118] (ab256494)

CD10 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab256494 at 1/30 (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab256494 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate 10ug

Lane 2: ab256494 IP in Raji whole cell lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab256494 in Raji whole cell lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 min
Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunofluorescent analysis of 100% Methanol-fixed 2.4G2 (rat B cell lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by Ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in 2.4G2 cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunofluorescent analysis of 100% Methanol-fixed WEHI-231 (mouse B cell lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by Ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing staining in WEHI-231 cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control: Secondary antibody is Ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunofluorescent analysis of 100% Methanol-fixed, Ramos (human Burkitt's lymphoma B lymphocyte) cells labelling CD10 with ab256494 at 1/500 dilution, followed by Ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green).Confocal image showing membranous staining in Ramos cell line is observed. Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue). Negative control: HT-29 （PMID: 19828468）

Secondary antibody only control: Secondary antibody is Ab256494 anti- CD10 ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on renal tubules and of rat kidney (PMID:10705818) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunohistochemical analysis of paraffin-embedded Mouse kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on renal tubules and of mouse kidney (PMID:10705818) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous staining on syncytiotrophoblast layer of human placenta (PMID:11092533) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [EPR22867-118] (ab256494)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab256494 at 1/500 dilution followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Positive staining on proximal convoluted tubules and glomerular epithelial cells of human kidney (PMID:10705818) is observed. Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Anti-CD10 antibody [EPR22867-118] (ab256494)

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

SDS download

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Datasheet download

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Publishing research using ab256494? Please let us know so that we can cite the reference in this datasheet.

ab256494 has been referenced in 6 publications.

Chen Q et al. Rho-kinase inhibitor hydroxyfasudil protects against HIV-1 Tat-induced dysfunction of tight junction and neprilysin/Aß transfer receptor expression in mouse brain microvessels. Mol Cell Biochem 476:2159-2170 (2021). PubMed: 33548010
Wang L et al. PTH-induced EndMT via miR-29a-5p/GSAP/Notch1 pathway contributed to valvular calcification in rats with CKD. Cell Prolif 54:e13018 (2021). PubMed: 33945189
Zhang S et al. The Hippo Effector Transcriptional Coactivator with PDZ-Binding Motif Cooperates with Oncogenic ß-Catenin to Induce Hepatoblastoma Development in Mice and Humans. Am J Pathol 190:1397-1413 (2020). PubMed: 32283103
Wen S et al. Whole-Exome Sequencing Reveals New Potential Mutations Genes for Primary Mucosa-Associated Lymphoid Tissue Lymphoma Arising From the Kidney. Front Oncol 10:609839 (2020). PubMed: 33585230
Khaleefah MM et al. Isolated oculomotor nerve palsy as a manifestation of diffuse large B cell lymphoma: A case report. Oncol Lett 20:285 (2020). PubMed: 33014163
Song Z et al. Comprehensive Proteomic Profiling of Urinary Exosomes and Identification of Potential Non-invasive Early Biomarkers of Alzheimer's Disease in 5XFAD Mouse Model. Front Genet 11:565479 (2020). PubMed: 33250918

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293T)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Treatment

CD10-transfected

Specification

HEK293T

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

Submitted Nov 12 2021

Application

Immunocytochemistry

Sample

Human Cultured Cells (Melanoma cells)

Permeabilization

Specification

Melanoma cells

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Apr 16 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Skin)

Permeabilization

Specification

Skin

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 22 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (Fibroblasts)

Permeabilization

Specification

Fibroblasts

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: RT°C

Fixative

Acetone

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 14 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Scar tissue)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: EDTA

Permeabilization

Specification

Scar tissue

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 01 2020

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (6)

Customer reviews and Q&As

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Western blot abreview for Anti-CD10 antibody [EPR22867-118]

Immunocytochemistry abreview for Anti-CD10 antibody [EPR22867-118]

Immunohistochemistry (Frozen sections) abreview for Anti-CD10 antibody [EPR22867-118]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD10 antibody [EPR22867-118]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD10 antibody [EPR22867-118]