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    products/primary-antibodies/cd105-antibody-epr10145-10-ab170943.pdf

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Immunology Cell Type Markers CD Endothelial Cells
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD105 antibody [EPR10145-10] (ab170943)

  • Datasheet
  • SDS
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Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Anti-CD105 antibody [EPR10145-10] (ab170943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10145-10] to CD105
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

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Knockout
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Human ENG (CD105) knockout HeLa cell line (ab265178)

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Overview

  • Product name

    Anti-CD105 antibody [EPR10145-10]
    See all CD105 primary antibodies
  • Description

    Rabbit monoclonal [EPR10145-10] to CD105
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human CD105 aa 1-200. The exact sequence is proprietary.
    Database link: P17813

  • Positive control

    • WB: HeLa, HUVEC, and ECV-304 whole cell lysates. IHC-P: Human lung carcinoma, urinary bladder transitional carcinoma, cervical carcinoma, tonsil, uterus and placenta tissues.
  • General notes

    According to our internal WB data, ab170943 does not react with mouse and rat.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR10145-10
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Anti-CD105 antibody [EPR10145-10] - BSA and Azide free (ab249611)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human ENG (CD105) knockout HeLa cell line (ab265178)
  • KO cell lysates

    • Human ENG (CD105) knockout HeLa cell lysate (ab256906)
  • Recombinant Protein

    • Recombinant human CD105 protein (ab54338)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab170943 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P17813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2022 Human
    • Omim: 131195 Human
    • SwissProt: P17813 Human
    • Unigene: 76753 Human
    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ENG knockout HeLa cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 70-120 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab170943 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab170943 Anti-CD105 antibody [EPR10145-10] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab170943 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : CD105 knockout HeLa whole cell lysate
      Lane 3 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 70 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab170943 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab170943 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105  knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab170943 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human lung carcinoma-vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human normal tonsil-vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human urinary bladder transitional carcinoma, vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human normal uterus, vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD105 with ab170943  at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD105 with ab170943 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : ECV-304 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa

    • Anti-CD105 antibody [EPR10145-10] (ab170943)
      Anti-CD105 antibody [EPR10145-10] (ab170943)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab170943? Please let us know so that we can cite the reference in this datasheet.

    ab170943 has been referenced in 3 publications.

    • Piao L  et al. Leucine Zipper-EF-Hand Containing Transmembrane Protein 1 Is a Potential Prognostic Biomarker and Promotes Cell Progression in Prostate Cancer. Cancer Manag Res 12:1649-1660 (2020). PubMed: 32184668
    • Piao L  et al. LETM1 is a potential biomarker of prognosis in lung non-small cell carcinoma. BMC Cancer 19:898 (2019). PubMed: 31500591
    • Bychkov A  et al. PSMA expression by microvasculature of thyroid tumors - Potential implications for PSMA theranostics. Sci Rep 7:5202 (2017). PubMed: 28701709

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