Recombinant Anti-CD105 antibody [EPR10145-10] (ab170943)
![Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-386666-anti-cd105-antibody-epr10145-10-western-blot-human-lysate.jpg "Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10145-10] to CD105
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD105 antibody [EPR10145-10]
See all CD105 primary antibodies -
Description
Rabbit monoclonal [EPR10145-10] to CD105 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Does not react with: Mouse, Rat -
Immunogen
Synthetic peptide within Human CD105 aa 1-200. The exact sequence is proprietary.
Database link: P17813 -
Positive control
- WB: HeLa, HUVEC, and ECV-304 whole cell lysates. IHC-P: Human lung carcinoma, urinary bladder transitional carcinoma, cervical carcinoma, tonsil, uterus and placenta tissues.
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General notes
According to our internal WB data, ab170943 does not react with mouse and rat.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10145-10 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170943 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
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| IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa. |
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IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ENG knockout HeLa cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab170943 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.
ab170943 Anti-CD105 antibody [EPR10145-10] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab170943 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-338202-anti-cd105-antibody-epr10145-10-western-blot.jpg)
Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lane 3 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaLanes 1 - 3: Merged signal (red and green). Green - ab170943 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab170943 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab170943 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-221498-anti-cd105-antibody-epr10145-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)ab170943 showing +ve staining in Human lung carcinoma-vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-221497-anti-cd105-antibody-epr10145-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)ab170943 showing +ve staining in Human normal tonsil-vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-221496-anti-cd105-antibody-epr10145-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)ab170943 showing +ve staining in Human urinary bladder transitional carcinoma, vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-221495-anti-cd105-antibody-epr10145-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)ab170943 showing +ve staining in Human normal uterus, vessels tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-171189-anti-cd105-antibody-epr10145-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD105 with ab170943 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-171188-anti-cd105-antibody-epr10145-10-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD105 with ab170943 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-171190-anti-cd105-antibody-epr10145-10-western-blot.jpg)
Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution
Lane 1 : HUVEC cell lysate
Lane 2 : ECV-304 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 70 kDa -
![Anti-CD105 antibody [EPR10145-10] (ab170943)](https://www.abcam.com/ps/products/170/ab170943/Images/ab170943-15-benefits-of-recombinant-antibodies.png)
Anti-CD105 antibody [EPR10145-10] (ab170943)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab170943 has been referenced in 3 publications.
- Piao L et al. Leucine Zipper-EF-Hand Containing Transmembrane Protein 1 Is a Potential Prognostic Biomarker and Promotes Cell Progression in Prostate Cancer. Cancer Manag Res 12:1649-1660 (2020). PubMed: 32184668
- Piao L et al. LETM1 is a potential biomarker of prognosis in lung non-small cell carcinoma. BMC Cancer 19:898 (2019). PubMed: 31500591
- Bychkov A et al. PSMA expression by microvasculature of thyroid tumors - Potential implications for PSMA theranostics. Sci Rep 7:5202 (2017). PubMed: 28701709