Recombinant Anti-CD105 antibody [EPR10145-12] (ab169545)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10145-12] to CD105
- Suitable for: WB, IHC-P
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-CD105 antibody [EPR10145-12]
See all CD105 primary antibodies -
Description
Rabbit monoclonal [EPR10145-12] to CD105 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD105 aa 1-200.
Database link: P17813 -
Positive control
- WB: HeLa, ECV-304 and HUVEC cell lysates; Human tonsil tissue lysate; Immunoprecipitation pellet from ECV-304 cell lysate. IHC-P: Human glioma, clear cell carcinoma, tonsil and kidney tissues.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10145-12 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
KO cell lines
-
KO cell lysates
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab169545 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/2000. Predicted molecular weight: 70 kDa.
For unpurified use at 1/50. |
IHC-P | (2) |
1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/30. |
Notes |
---|
WB
1/1000 - 1/2000. Predicted molecular weight: 70 kDa. For unpurified use at 1/50. |
IHC-P
1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30. |
Target
-
Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
-
Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
-
All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ENG knockout HeLa cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : MCF7 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 70 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?Lanes 1-4: Merged signal (red and green). Green - ab169545 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.
ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lane 3 : HUVEC whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 70 kDaLanes 1 - 3: Merged signal (red and green). Green - ab169545 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab169545 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab169545 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with unpurified ab169545 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
-
All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified)
Lane 1 : ECV-304 cell lysate
Lane 2 : Human tonsil cell lysate
Lane 3 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 70 kDa -
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + ECV-304 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + ECV-304 cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified) + immunoprecipitation pellet from ECV-304 cell lysate
Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG
Predicted band size: 70 kDa -
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + HUVEC cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + HUVEC cell lysate at 10 µg
Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with purified ab169545 at 1/900. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human clear cell carcinoma tissue labelling CD105 with unpurified ab169545 at 1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling CD105 with unpurified ab169545 at 1/250.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD105 with unpurified ab169545 at 1/250.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab169545 has been referenced in 15 publications.
- Fattahi F et al. Expressions of TWIST1 and CD105 markers in colorectal cancer patients and their association with metastatic potential and prognosis. Diagn Pathol 16:26 (2021). PubMed: 33752711
- Huang J et al. Myocardial infarction cardiomyocytes-derived exosomal miR-328-3p promote apoptosis via Caspase signaling. Am J Transl Res 13:2365-2378 (2021). PubMed: 34017395
- Sharma R et al. Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. J Exp Clin Cancer Res 40:186 (2021). PubMed: 34099013
- Chun J et al. Osteogenic differentiation and inflammatory response of recombinant human bone morphogenetic protein-2 in human maxillary sinus membrane-derived cells. Exp Ther Med 20:81 (2020). PubMed: 32968438
- Su C et al. The effective role of sodium copper chlorophyllin on the dysfunction of bone marrow mesenchymal stem cells in multiple myeloma via regulating TGF-ß1. Tissue Cell 67:101406 (2020). PubMed: 32835939