Name: Anti-CD105 antibody [EPR10145-12]
Brand: RabMAb
SKU: ab169545
Rating: 4 (3 reviews)

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Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10145-12] to CD105
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-CD105 antibody [EPR10145-12]
See all CD105 primary antibodies
Description

Rabbit monoclonal [EPR10145-12] to CD105
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-Pmore details
Species reactivity

Reacts with: Human
Immunogen

Recombinant fragment corresponding to Human CD105 aa 1-200.
Database link: P17813
Positive control
- WB: HeLa, ECV-304 and HUVEC cell lysates; Human tonsil tissue lysate; Immunoprecipitation pellet from ECV-304 cell lysate. IHC-P: Human glioma, clear cell carcinoma, tonsil and kidney tissues.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10145-12
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD105 antibody [EPR10145-12] - Low endotoxin, Azide free (ab219362)
- Anti-CD105 antibody [EPR10145-12] - BSA and Azide free (ab271922)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
KO cell lines
- Human ENG (CD105) knockout HeLa cell line (ab265178)
KO cell lysates
- Human ENG (CD105) knockout HeLa cell lysate (ab256906)
Recombinant Protein
- Recombinant human CD105 protein (ab54338)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab169545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/2000. Predicted molecular weight: 70 kDa. For unpurified use at 1/50.
IHC-P	(2)	1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30.

Notes
WB 1/1000 - 1/2000. Predicted molecular weight: 70 kDa. For unpurified use at 1/50.
IHC-P 1/900. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/30.

Function

Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
Tissue specificity

Endoglin is restricted to endothelial cells in all tissues except bone marrow.
Involvement in disease

Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
Cellular localization

Membrane.
Target information above from: UniProt accession P17813 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
- CD105 antibody
- CD105 antigen antibody
- EGLN_HUMAN antibody
- END antibody
- Endoglin antibody
- Eng antibody
- FLJ41744 antibody
- HHT1 antibody
- ORW antibody
- ORW1 antibody
- Osler Rendu Weber syndrome 1 antibody
- RP11 228B15.2 antibody
- S endoglin antibody
- S-endoglin antibody
- SN6 antibody
see all

Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ENG knockout HeLa cell lysate
Lane 3 : HUVEC cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 70 kDa
Observed band size: 70-120 kDa why is the actual band size different from the predicted?

Lanes 1-4: Merged signal (red and green). Green - ab169545 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.

ab169545 Anti-CD105 antibody [EPR10145-12] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab169545 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution

Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : CD105 knockout HeLa whole cell lysate
Lane 3 : HUVEC whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 70 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab169545 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab169545 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab169545 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with unpurified ab169545 at 1/30. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

All lanes : Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified)

Lane 1 : ECV-304 cell lysate
Lane 2 : Human tonsil cell lysate
Lane 3 : HUVEC cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution

Predicted band size: 70 kDa
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + ECV-304 cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + ECV-304 cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1000 dilution (unpurified) + immunoprecipitation pellet from ECV-304 cell lysate

Secondary
HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG

Predicted band size: 70 kDa
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/50 dilution (unpurified) + HUVEC cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-CD105 antibody [EPR10145-12] (ab169545)

Anti-CD105 antibody [EPR10145-12] (ab169545) at 1/1200 dilution (purified) + HUVEC cell lysate at 10 µg

Secondary
Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD105 with purified ab169545 at 1/900. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis human clear cell carcinoma tissue labelling CD105 with unpurified ab169545 at 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human glioma tissue labelling CD105 with unpurified ab169545 at 1/250.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-12] (ab169545)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling CD105 with unpurified ab169545 at 1/250.
Anti-CD105 antibody [EPR10145-12] (ab169545)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab169545? Please let us know so that we can cite the reference in this datasheet.

ab169545 has been referenced in 15 publications.

Fattahi F et al. Expressions of TWIST1 and CD105 markers in colorectal cancer patients and their association with metastatic potential and prognosis. Diagn Pathol 16:26 (2021). PubMed: 33752711
Huang J et al. Myocardial infarction cardiomyocytes-derived exosomal miR-328-3p promote apoptosis via Caspase signaling. Am J Transl Res 13:2365-2378 (2021). PubMed: 34017395
Sharma R et al. Determinants of resistance to VEGF-TKI and immune checkpoint inhibitors in metastatic renal cell carcinoma. J Exp Clin Cancer Res 40:186 (2021). PubMed: 34099013
Chun J et al. Osteogenic differentiation and inflammatory response of recombinant human bone morphogenetic protein-2 in human maxillary sinus membrane-derived cells. Exp Ther Med 20:81 (2020). PubMed: 32968438
Su C et al. The effective role of sodium copper chlorophyllin on the dysfunction of bone marrow mesenchymal stem cells in multiple myeloma via regulating TGF-ß1. Tissue Cell 67:101406 (2020). PubMed: 32835939

View all Publications for this product

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing

Sample

Human Cell lysate - whole cell (Normal Human Keratinocyte)

Specification

Normal Human Keratinocyte

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 30 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval

Sample

Cynomolgus Monkey Tissue sections (Bone marrow)

Specification

Bone marrow

Permeabilization

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 26 2014

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: 10mm sodium citrate PH 6

Sample

Human Tissue sections (breast)

Specification

breast

Permeabilization

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Aug 22 2013

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

KO cell lines

KO cell lysates

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (15)

Customer reviews and Q&As

Western blot abreview for Anti-CD105 antibody [EPR10145]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD105 antibody [EPR10145]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD105 antibody [EPR10145]