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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-CD105 antibody [EPR19911] (ab206419)

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Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
  • Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)
  • Anti-CD105 antibody [EPR19911] (ab206419)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19911] to CD105
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CD105 antibody [EPR19911]
    See all CD105 primary antibodies

  • Description

    Rabbit monoclonal [EPR19911] to CD105

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: THP-1 and HUVEC whole cell lysates; human fetal kidney lysate. IHC-P: Human tonsil and kidney cancer tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab206419 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000. Detects a band of approximately 95, 90 kDa (predicted molecular weight: 70 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000. Detects a band of approximately 95, 90 kDa (predicted molecular weight: 70 kDa).
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.

  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.

  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
      Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
      All lanes : Anti-CD105 antibody [EPR19911] (ab206419) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : CD105  knockout HeLa whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 70 kDa



      Lanes 1 - 2: Merged signal (red and green). Green - ab206419 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab206419 was shown to recognize CD105  in wild-type HeLa cells as signal was lost at the expected MW in CD105  knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105  knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab206419 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
      Western blot - Anti-CD105 antibody [EPR19911] (ab206419)
      All lanes : Anti-CD105 antibody [EPR19911] (ab206419) at 1/1000 dilution

      Lane 1 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
      Lane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
      Lane 3 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lane 3 : Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution

      Predicted band size: 70 kDa
      Observed band size: 90,95 kDa why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Dilution buffer: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 1692830, PMID: 18223685, PMID: 11164421).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)

      Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Cytoplasm on human tonsil vascular endothelium is observed [PMID:17502949].

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR19911] (ab206419)

      Immunohistochemical analysis of paraffin-embedded human kidney cancer tissue labeling CD105 with ab206419 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

      Cytoplasm on human kidney vascular endothelium is observed.

      Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Anti-CD105 antibody [EPR19911] (ab206419)
      Anti-CD105 antibody [EPR19911] (ab206419)

    References (0)

    Publishing research using ab206419? Please let us know so that we can cite the reference in this datasheet.

    ab206419 has not yet been referenced specifically in any publications.

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