Recombinant Anti-CD105 antibody [EPR21846] (ab221675)
![Flow Cytometry - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302218-anti-cd105-antibody-epr21846-flow-cytometry.jpg "Flow Cytometry - Anti-CD105 antibody [EPR21846] (ab221675)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21846] to CD105
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IP, Flow Cyt
- Reacts with: Mouse
Related conjugates and formulations
Overview
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Product name
Anti-CD105 antibody [EPR21846]
See all CD105 primary antibodies -
Description
Rabbit monoclonal [EPR21846] to CD105 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: bEND.3 whole cell lysate; Mouse lung lysate. IHC-P: Mouse lung and E14.5 liver tissues. IHC-Fr: Mouse liver and brain tissues. ICC/IF: bEND.3 cells. Flow Cyt: bEND.3 cells. IP: bEND.3 whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21846 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab221675 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr |
1/50.
Perform heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681). |
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| ICC/IF |
1/1000.
|
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| IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
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| WB |
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 70 kDa).
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| IP |
1/30.
|
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| Flow Cyt |
1/500.
|
| Notes |
|---|
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IHC-Fr
1/50. Perform heat mediated antigen retrieval by using Tris-EDTA buffer (pH9.0) (ab94681). |
|
ICC/IF
1/1000. |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
|
WB
1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 70 kDa). |
|
IP
1/30. |
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Flow Cyt
1/500. |
Target
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Function
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
Tissue specificity
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
Involvement in disease
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 13805 Mouse
- SwissProt: Q63961 Mouse
- Unigene: 225297 Mouse
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Alternative names
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
Images
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Flow Cytometry - Anti-CD105 antibody [EPR21846] (ab221675)
Flow cytometric analysis of NIH/3T3 (mouse embryo fibroblast cell line) cell line (left panel) and bEND.3 (mouse brain endothelioma cell line) cell line (right panel) labeling CD105 with ab221675 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on total viable cells.
Negative control: NIH/3T3. (PMID: 8194490).
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![Western blot - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302217-anti-cd105-antibody-epr21846-western-blot.jpg)
Western blot - Anti-CD105 antibody [EPR21846] (ab221675)All lanes : Anti-CD105 antibody [EPR21846] (ab221675) at 1/1000 dilution
Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 70 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
Exposure time: 70 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular mass is consistent with the literature (PMID: 8932339). Negative control: NIH/3T3 (PMID: 8194490).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302215-anti-cd105-antibody-epr21846-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (ab221675)Immunohistochemical analysis of paraffin-embedded mouse E14.5 liver tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse E14.5 liver is observed (PMID: 18805961). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302216-anti-cd105-antibody-epr21846-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (ab221675)Immunohistochemical analysis of paraffin-embedded mouse lung tissue labeling CD105 with ab221675 at 1/100 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use. Positive staining on endothelial cells of mouse lung is observed (PMID: 14528280). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101), Ready to use.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302473-anti-cd105-antibody-epr21846-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD105 antibody [EPR21846] (ab221675)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution (green). Positive staining in the hepatic sinusoidal endothelial cells on mouse liver tissue section (PMID: 12947156; 24507660). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302474-anti-cd105-antibody-epr21846-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR21846] (ab221675)Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse brain tissue labeling CD105 with ab221675 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution. Positive staining in the endothelial cells of blood vessels in mouse brain tissue section (PMID: 24699047). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/500 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302214-anti-cd105-antibody-epr21846-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR21846] (ab221675)Immunofluorescent analysis of 100% methanol-fixed bEND.3 (mouse brain endothelioma cell line) cells labeling CD105 with ab221675 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in Neuro-2a cell line. Negative control: NIH/3T3 (PMID: 8194490).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Immunoprecipitation - Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-302219-anti-cd105-antibody-epr21846-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD105 antibody [EPR21846] (ab221675)CD105 was immunoprecipitated from 0.35 mg of bEND.3 (mouse brain endothelioma cell line) whole cell lysate with ab221675 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab221675 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/5000 dilution.
Lane 1: bEND.3 whole cell lysate 10 µg (Input).
Lane 2: ab221675 IP in bEND.3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab221675 in bEND.3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 5 seconds.
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![Anti-CD105 antibody [EPR21846] (ab221675)](https://www.abcam.com/ps/products/221/ab221675/Images/ab221675-9-benefits-of-recombinant-antibodies.png)
Anti-CD105 antibody [EPR21846] (ab221675)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab221675 has been referenced in 2 publications.
- Walker DG et al. Microglial Phenotyping in Neurodegenerative Disease Brains: Identification of Reactive Microglia with an Antibody to Variant of CD105/Endoglin. Cells 8:N/A (2019). PubMed: 31340569
- Li AD et al. miR-124 regulates cerebromicrovascular function in APP/PS1 transgenic mice via C1ql3. Brain Res Bull 153:214-222 (2019). PubMed: 31499089