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RecombinantRabMAb

Recombinant Anti-CD105 antibody [EPR22811-18] (ab231774)

Reviews (1) Submit a question References (3)
Western blot - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Western blot - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Immunoprecipitation - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Flow Cytometry - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Flow Cytometry - Anti-CD105 antibody [EPR22811-18] (ab231774)
  • Anti-CD105 antibody [EPR22811-18] (ab231774)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22811-18] to CD105
  • Suitable for: Flow Cyt, ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Overview

  • Product name

    Anti-CD105 antibody [EPR22811-18]
    See all CD105 primary antibodies

  • Description

    Rabbit monoclonal [EPR22811-18] to CD105

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IP, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC and U937 whole cell lysate; Human lung and placenta tissue lysate. IHC-P: Human ovarian carcinoma and placenta tissue. ICC/IF: U937 cells. Flow Cyt: HUVEC and U937 cells. IP: HUVEC whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab231774 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
ICC/IF
1/500.
IP
1/30.
IHC-P (1)
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 70 kDa.
Notes
Flow Cyt
1/500.
ICC/IF
1/500.
IP
1/30.
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB
1/1000. Predicted molecular weight: 70 kDa.

Target

  • Function

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.

  • Tissue specificity

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.

  • Involvement in disease

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    Images

    • Western blot - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Western blot - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Anti-CD105 antibody [EPR22811-18] (ab231774) at 1/1000 dilution + U937 (human histiocytic lymphoma monocyte), whole cell lysate 40 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 70 kDa
      Observed band size: 97 kDa why is the actual band size different from the predicted?


      Exposure time: 103 seconds


      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 8932339).

    • Western blot - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Western blot - Anti-CD105 antibody [EPR22811-18] (ab231774)
      All lanes : Anti-CD105 antibody [EPR22811-18] (ab231774) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate 20 µg
      Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 20 µg
      Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate 20 µg
      Lane 4 : Human lung tissue lysate 20 µg
      Lane 5 : Human placenta tissue lysate 20 µg

      Secondary
      Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
      Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution

      Predicted band size: 70 kDa



      Blocking/Diluting buffer and concentration: 5% NFDM/TBST.

      Exposure times: Lanes 1-3: 26 secs; Lanes 4-5: 3 mins.

      The molecular weight observed is consistent with what has been described in the literature (PMID: 8932339).

      This blot was developed using a higher sensitivity ECL substrate.

      Negative control: Jurkat (PMID: 28351936); Raji (PMID: 28351936).

    • Immunoprecipitation - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Immunoprecipitation - Anti-CD105 antibody [EPR22811-18] (ab231774)

      CD105 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab231774 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab231774 1/1000 dilution (0.51 μg/ml). VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

      Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10μg
      Lane 2: ab231774 IP in HUVEC whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab231774 in HUVEC whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 3 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] (ab231774)

      Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling CD105 with ab231774 at 1/100 dilution (5.1 μg/ml)  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human ovarian carcinoma (PMID: 17502949). The section was incubated with ab231774 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR22811-18] (ab231774)

      Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD105 with ab231774 at 1/100 dilution (5.1 μg/ml)  followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placental trophoblasts (PMID: 17956952) is observed. The section was incubated with ab231774 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [EPR22811-18] (ab231774)

      Immunofluorescent analysis of 100% methanol-fixed U-937 (human histiocytic lymphoma monocyte) cells labeling CD105 with ab231774 at 1/500 dilution, followed by a ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in U-937 cells is observed. ab195889  Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)  was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      Negative control: Jurkat (PMID: 28351936).

    • Flow Cytometry - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Flow Cytometry - Anti-CD105 antibody [EPR22811-18] (ab231774)

      Flow cytometric analysis of U-937 (human histiocytic lymphoma monocyte) cells labeling CD105 with ab231774 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells. 

    • Flow Cytometry - Anti-CD105 antibody [EPR22811-18] (ab231774)
      Flow Cytometry - Anti-CD105 antibody [EPR22811-18] (ab231774)

      Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labeling CD105 with ab231774 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: Jurkat (PMID: 28351936). Gated on viable cells.

    • Anti-CD105 antibody [EPR22811-18] (ab231774)
      Anti-CD105 antibody [EPR22811-18] (ab231774)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab231774? Please let us know so that we can cite the reference in this datasheet.

    ab231774 has been referenced in 3 publications.

    • Ye G  et al. Autophagy-Mediated Activation of Mucosal-Associated Invariant T Cells Driven by Mesenchymal Stem Cell-Derived IL-15. Stem Cell Reports 16:926-939 (2021). PubMed: 33798448
    • Wangzhou K  et al. MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/ß-Catenin Pathway. Front Physiol 11:606967 (2020). PubMed: 33603676
    • Ye G  et al. Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis. Cell Death Dis 11:775 (2020). PubMed: 32943613

    Customer reviews and Q&As

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD105 antibody [EPR22811-18]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (FFPE Heart section)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH6 and pH9
    Permeabilization
    No
    Specification
    FFPE Heart section
    Blocking step
    H2O2 as blocking agent for 7 minute(s) · Concentration: 3% · Temperature: 24°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    In Hwa Um

    Verified customer

    Submitted Jun 29 2020

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