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    products/primary-antibodies/cd11b-antibody-ep1345y-c-terminal-ab52478.pdf

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Signal Transduction Cytoskeleton / ECM Cell Adhesion Integrins Alpha
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RecombinantRabMAb

Recombinant Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

  • Datasheet
  • SDS
Reviews (5)Q&A (2)References (53)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Immunoprecipitation - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
  • Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1345Y] to CD11b - C-terminal
  • Suitable for: ICC/IF, IHC-FoFr, WB, IP, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-CD11b antibody [EP1345Y] - C-terminal
    See all CD11b primary antibodies
  • Description

    Rabbit monoclonal [EP1345Y] to CD11b - C-terminal
  • Host species

    Rabbit
  • Specificity

    Testing of mouse and rat tissues (brain, spleen, kidney and heart) in WB gave negative results. However, flow cytometry for mouse RAW 264.7 cell line gave positive results. We have not tested any rat samples in flow cytometry. Due to the variability in mouse, we do not list this as a tested species. We welcome any feedback on mouse and rat reactivity.
  • Tested applications

    Suitable for: ICC/IF, IHC-FoFr, WB, IP, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CD11b aa 1100 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P11215

  • Positive control

    • IHC-P: Human spleen and human cervical cancer tissue WB: TF1 lysate. THP-1 macrophages, +10 ng/ml LPS. ICC/IF: THP-1 cell lysate IP: TF-1 whole cell lysate
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1345Y
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Cell Adhesion
    • Integrins
    • Alpha
    • Tags & Cell Markers
    • Cell Type Markers
    • Neuroscience Markers
    • Glial
    • Neuroscience
    • Cell Type Marker
    • Glia marker
    • Microglia marker
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • Murine Lineage Negative
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-CD11b antibody [EP1345Y] - BSA and Azide free (ab187537)
    • PE Anti-CD11b antibody [EP1345Y] (ab213186)
  • Compatible Secondaries

    • VeriBlot for IP Detection Reagent (HRP) (ab131366)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD11b protein (ab126010)
  • Related Products

    • Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab52478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100 - 1/250.
IHC-FoFr (1)
Use at an assay dependent concentration.
WB (2)
1/1000. Predicted molecular weight: 128 kDa.

For unpurified use at 1/20000 - 1/50000

IP
1/30.

For unpurified use at 1/80

IHC-P (1)
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1 - 5 µg/ml

Notes
ICC/IF
1/100 - 1/250.
IHC-FoFr
Use at an assay dependent concentration.
WB
1/1000. Predicted molecular weight: 128 kDa.

For unpurified use at 1/20000 - 1/50000

IP
1/30.

For unpurified use at 1/80

IHC-P
1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1 - 5 µg/ml

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.
  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P11215 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 3684 Human
    • Omim: 120980 Human
    • SwissProt: P11215 Human
    • Unigene: 172631 Human
    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical cancer tissue sections labeling CD11b with purified ab52478 at 1:1000 dilution (0.28 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Hematoxylin was used as a counterstain.

      Negative control: PBS instead of the primary antibody (inset).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

      Unpurified ab52478 staining CD11b in the THP-1 (Human monocytic leukemia cell line) cell line by ICC/IF (Immunocytochemistry/immunofluorescence).

      Cells were fixed with 100% methanol. Samples were incubated with primary antibody (1/250). ab150077 was used as the secondary antibody (1/1000). Nuclei were stained with DAPI.

    • Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) at 0.3 µg/ml (purified) + TF-1 (Human Erythroleukemia erythroblast) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 128 kDa



      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunoprecipitation - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunoprecipitation - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

      ab52478 (purified) at 1:30 dilution (2 µg) immunoprecipitating CD11b in TF-1 (Human bone marrow erythroleukemia cell line) whole cell lysate.
      Lane 1: TF-1 whole cell lysate 10 µg (input). 
      Lane 2: ab52478 + TF-1 whole cell lysate
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab52478 in TF-1 whole cell lysate
      For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
      Blocking and diluting buffer: 5% NFDM/TBST.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)Sindhu et al PLoS One. 2015 Jul 22;10(7):e0133494. doi: 10.1371/journal.pone.0133494. eCollection 2015. Fig 5. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Enhanced expression of monocytes/ macrophage markers in the obese adipose tissue.

      The protein expression (intensity) of monocyte/ macrophage markers was detected by immunohistochemistry (IHC) in the adipose tissue samples from lean, overweight, and obese individuals, 10 each. As shown by representative IHC photomicrographs (100× magnification), expression of (A) CD11b was found to be markedly elevated in overweight and obese adipose tissue samples as compared with lean samples.

      Paraffin-embedded sections (4 μm thick) of subcutaneous adipose tissue were deparaffinized in xylene and rehydrated through descending grades of ethanol (100%, 95%, and 75%) to water. Antigen retrieval was performed under pressure cooker boiling for 8 min and cooling for 15 min. After washing in PBS, endogenous peroxidase activity was blocked with 3% H2O2 for 30 min and non-specific antibody binding was clocked with 5% nonfat milk for 1hr and 1% bovine serum albumin (BSA) solution for 1hr. Slides were treated overnight with primary antibodies at room temperature. After washing with PBS (0.5% Tween), slides were incubated for 1hr with secondary antibody conjugated with HRP polymer chain and color was developed using 3,3ʹ-diaminobenzidine chromogen substrate. Specimens were washed in running tap water, lightly counterstained with hematoxylin, dehydrated through ascending grades of ethanol (75%, 95%, and 100%), cleared in xylene, and finally mounted in dibutyl phthalate xylene (DPX).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)Martin et al PLoS One. 2014 Feb 5;9(2):e88226. doi: 10.1371/journal.pone.0088226. eCollection 2014. Fig 6. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

      Duchenne muscular dystrophy (DMD) muscle was co-stained for Neu5Gc (green), ab52478 (red) and DAPI (blue).

      For double immunostaining, sections were first stained overnight at 4°C with anti-Neu5Gc after blocking in 10% (Neu5Gc-free) human serum, after blocking in 5 mg/mL BSA, sections were incubated overnight with both primary antibodies without fixation, washed for one hour and incubated with the appropriate secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

      IHC image of CD11b staining in a formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F.

      The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6, epitope retrieval solution 1) for 20 minutes. The section was then incubated with unpurified ab52478, 5 µg/ml, for 15 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

       

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

    • Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Western blot - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478) at 1/20000 dilution (unpurified) + TF-1 (Human bone marrow erythroleukemia cell line) lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 128 kDa
      Observed band size: 170 kDa why is the actual band size different from the predicted?

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue using unpurified ab52478 at a dilution of 1/100.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)
      Anti-CD11b antibody [EP1345Y] - C-terminal (ab52478)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (53)

    Publishing research using ab52478? Please let us know so that we can cite the reference in this datasheet.

    ab52478 has been referenced in 53 publications.

    • Chen SJ  et al. Targeting lysosomal cysteine protease cathepsin S reveals immunomodulatory therapeutic strategy for oxaliplatin-induced peripheral neuropathy. Theranostics 11:4672-4687 (2021). PubMed: 33754020
    • Keren L  et al. MIBI-TOF: A multiplexed imaging platform relates cellular phenotypes and tissue structure. Sci Adv 5:eaax5851 (2019). Mass Cytometry . PubMed: 31633026
    • Ru W  et al. Microglia Mediate HIV-1 gp120-Induced Synaptic Degeneration in Spinal Pain Neural Circuits. J Neurosci 39:8408-8421 (2019). PubMed: 31471472
    • Li D  et al. ß-1,3-Glucan/CR3/SYK pathway-dependent LC3B-II accumulation enhanced the fungicidal activity in human neutrophils. J Microbiol N/A:N/A (2019). PubMed: 30721460
    • Liu W & Rask-Andersen H Super-resolution immunohistochemistry study on CD4 and CD8 cells and the relation to macrophages in human cochlea. J Otol 14:1-5 (2019). PubMed: 30936894
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD11b antibody [EP1345Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (TUBO Breast Cancer Tumor)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    Yes - 0.1% Triton X-100
    Specification
    TUBO Breast Cancer Tumor
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5%
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jun 22 2017

    Western blot abreview for Anti-CD11b antibody [EP1345Y]

    Excellent
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    10 µg
    Specification
    THP-1
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Mar 27 2017

    Immunohistochemistry (Frozen sections) abreview for Anti-CD11b antibody [EP1345Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (human cells grown on interphase co-culture)
    Permeabilization
    No
    Specification
    human cells grown on interphase co-culture
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted May 24 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-CD11b antibody [EP1345Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    Yes - 3% Triton X 100
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Apr 08 2013

    Western blot abreview for Anti-CD11b antibody [EP1345Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (THP-1 (human monocytic leukemia))
    Loading amount
    40 µg
    Specification
    THP-1 (human monocytic leukemia)
    Treatment
    200 nM PMA for 72 hours (THP-1 macrophages)
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 07 2012

    Question

    Product Anti-CD11b antibody [EP1345Y] (ab52478) is not listed to be reactive with mouse samples, but the datasheet shows an image of Flow Cytometry performed using mouse RAW 264.7 cells (mouse). Is ab52478 reactive with mouse samples?

    Is ab52478 reactive with rat samples?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 28 2012

    Answer

    Product Anti-CD11b antibody [EP1345Y] (ab52478) is currently validated for use with human samples types, and is covered by our Abpromise Guarantee only for use with human sample types in the applications listed on the datasheet.

    Testing of ab52478 with mouse and rat tissue samples (brain, spleen, kidney, and heart) in WB applications produced negative results. However, we did obtain positive results for use of this Ab in Flow Cytometry applications with the mouse RAW 264.7 cell line. Because of this variability in results when ab52478 is used with mouse samples, we do not at this time list mouse as a validated species for ab52478. Likewise, we do not recommend use of ab52478 with rat samples.

    Additional information regarding our Abpromise Guarantee can be found here: Abpromise Guarantee. Read More

    Abcam Scientific Support

    Answered on Mar 28 2012

    Question

    For use of ab52478 in Flow Cytometry with RAW 246.7 cells, how many cells were used, and in what final incubation volume?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2011

    Answer

    For Flow Cytometry experiments with ab52478 using RAW 246.7 cells were performed in a 100uL volume, using 1x10^6 cells.

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2011

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