Recombinant Anti-CD11b antibody [M1/70] (ab8878)
![Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)](https://www.abcam.com/ps/products/8/ab8878/Images/ab8878-501373-anti-cd11b-immunocytochemistry-raw-264-7.png "Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [M1/70] to CD11b
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD11b antibody [M1/70]
See all CD11b primary antibodies -
Description
Rat monoclonal [M1/70] to CD11b -
Host species
Rat -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cytometry: RAW 264.7 cells, Human peripheral blood mononuclear cell (PBMC), Mouse peripheral blood mononuclear cell (PBMC) IF/ICC: RAW 264.7 cells
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
M1/70 -
Myeloma
NS1 -
Isotype
IgG2b -
Light chain type
unknown -
Research areas
Associated products
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Alternative Versions
- Alexa Fluor® 488 Anti-CD11b antibody [M1/70] (ab197701)
- Alexa Fluor® 647 Anti-CD11b antibody [M1/70] (ab197702)
- PerCP/Cy5.5® Anti-CD11b antibody [M1/70] (ab210299)
- PE/Cy7® Anti-CD11b antibody [M1/70] (ab218786)
- violetFluor™ 450 Anti-CD11b antibody [M1/70] (ab242266)
- FITC Anti-CD11b antibody [M1/70] (ab24874)
- PE Anti-CD11b antibody [M1/70] (ab25175)
- redFluor™ 710 Anti-CD11b antibody [M1/70] (ab253091)
- APC Anti-CD11b antibody [M1/70] (ab25482)
- PE/Cy5® Anti-CD11b antibody [M1/70] (ab25533)
- Alexa Fluor® 594 Anti-CD11b antibody [M1/70] (ab312914)
- APC/Cy7® Anti-CD11b antibody [M1/70] (ab79096)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8878 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (4) |
1/50.
|
| Flow Cyt | (2) |
1/1000 - 1/10000.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
|
ICC/IF
1/50. |
|
Flow Cyt
1/1000 - 1/10000. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Target
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Function
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
Tissue specificity
Predominantly expressed in monocytes and granulocytes. -
Involvement in disease
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
Domain
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 3684 Human
- Entrez Gene: 16409 Mouse
- Omim: 120980 Human
- SwissProt: P11215 Human
- SwissProt: P05555 Mouse
- Unigene: 172631 Human
- Unigene: 262106 Mouse
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Alternative names
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD11b antibody [M1/70] (ab8878)
Confocal image showing membranous staining in RAW 264.7 cells.
RAW 264.7, WEHI-231 and THP-1 cells were fixed in 4% PFA and permeabilized with 0.1% Triton X-100. Primary antibody, ab8878 at 1:50 was incubated overnight at 4° C, followed by AlexaFluor® 488- conjugated Goat anti-Rat secondary antibody (ab150157) at 1/1000 dilution at RT for 45 min. ab179513 Anti-beta Tubulin, used as a counterstain at 1/200 dilution, was co-incubated with ab8878 overnight at 4° C, followed by Alexa Fluor® 594 Goat Anti-Rabbit secondary (ab150080) at 1/1000 dilution at RT for 45 min. Nucleus were visualized using DAPI.
Negative control: WEHI-231 (PMID: 2457584)
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![Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)](https://www.abcam.com/ps/products/8/ab8878/Images/ab8878-501379-anti-cd11b-flow-cytometry-wehi-231-raw-264-7.png)
Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)Flow Cytometry analysis of WEHI-231 (Mouse B cell lymphoma B lymphocyte, Left) / RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Right) cells stained for CD11b using AB8878 at a 1/1000 dilution (0.1 μg) (Red) compared to Rat monoclonal IgG - Isotype Control (Black) and cells without incubation with primary antibody and secondary antibody (Blue). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Negative control: WEHI-231.(PMID: 2457584)
Gated on viable cells.
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![Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)](https://www.abcam.com/ps/products/8/ab8878/Images/ab8878-501380-anti-cd11b-flow-cytometry-pbmc-human.png)
Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)Flow Cytometry analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD11b with AB8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
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![Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)](https://www.abcam.com/ps/products/8/ab8878/Images/ab8878-501382-anti-cd11b-flow-cytometry-pbmc-mouse.png)
Flow Cytometry - Anti-CD11b antibody [M1/70] (ab8878)Flow Cytometry analysis of Mouse peripheral blood mononuclear cell (PBMC) cells labelling CD11b with AB8878 at a 1/10,000 dilution (0.01 μg) (right) compared to Rat monoclonal IgG - Isotype Control (right). Goat F(ab)2 Anti-Rat IgG Fc (Alexa Fluor® 488, ab150161) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (138)
ab8878 has been referenced in 138 publications.
- Rana I et al. Mindin (SPON2) Is Essential for Cutaneous Fibrogenesis in a Mouse Model of Systemic Sclerosis. J Invest Dermatol 143:699-710.e10 (2023). PubMed: 36528128
- Gwak SY et al. Potential Role of Heme Oxygenase-1 in the Resolution of Experimentally Induced Colitis through Regulation of Macrophage Polarization. Gut Liver 16:246-258 (2022). PubMed: 34737242
- Zhou T et al. miR-204-containing exosomes ameliorate GVHD-associated dry eye disease. Sci Adv 8:eabj9617 (2022). PubMed: 35020440
- Lee HJ et al. Idebenone Regulates Aβ and LPS-Induced Neurogliosis and Cognitive Function Through Inhibition of NLRP3 Inflammasome/IL-1β Axis Activation. Front Immunol 13:749336 (2022). PubMed: 35222363
- Moudio S et al. Exposure of α-Synuclein Aggregates to Organotypic Slice Cultures Recapitulates Key Molecular Features of Parkinson's Disease. Front Neurol 13:826102 (2022). PubMed: 35309552