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RecombinantRabMAb

Recombinant Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Flow Cytometry - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
  • Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP330] to CD11b - BSA and Azide free
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alkaline Phosphatase APC HRP PE Unconjugated

Overview

  • Product name

    Anti-CD11b antibody [SP330] - BSA and Azide free
    See all CD11b primary antibodies

  • Description

    Rabbit monoclonal [SP330] to CD11b - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human tonsil, colon, spleen, bone marrow, lung, cerebellum, thymus, lymph node, HK lymphoma, pancreatic adenocarcinoma and endometrial adenocarcinoma tissues. Flow Cyt: TF-1 cells.

  • General notes

    ab241408 is the carrier-free version of ab224805.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab241408 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.

Primary incubation for 1 hour at room temperature.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
Flow Cyt
Use at an assay dependent concentration.

Primary incubation for 30 minutes at at 4°C.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 127 kDa.

Primary incubation for 1 hour at room temperature.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary incubation for 10 minutes at room temperature.
Flow Cyt
Use at an assay dependent concentration.

Primary incubation for 30 minutes at at 4°C.

Target

  • Function

    Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.

  • Tissue specificity

    Predominantly expressed in monocytes and granulocytes.

  • Involvement in disease

    Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.

  • Sequence similarities

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.

  • Domain

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • antigen CD11b (p170) antibody
      • Antigen CD11b p170 antibody
      • CD11 antigen like family member B antibody
      • CD11 antigen-like family member B antibody
      • CD11b antibody
      • CD11b/CD18 antibody
      • CD49d antibody
      • Cell surface glycoprotein MAC-1 subunit alpha antibody
      • Complement component 3 receptor 3 subunit antibody
      • Complement Component Receptor 3 Alpha antibody
      • Complement receptor type 3 antibody
      • Complement receptor type 3, alpha subunit antibody
      • CR 3 alpha chain (CR3A) antibody
      • CR 3 alpha chain antibody
      • CR-3 alpha chain antibody
      • CR3 antibody
      • CR3A antibody
      • F730045J24Rik antibody
      • Integrin Alpha M antibody
      • Integrin alpha M chain antibody
      • Integrin alpha-M antibody
      • Integrin beta 2 alpha subunit antibody
      • Integrin subunit alpha M antibody
      • integrin, alpha M (complement component 3 receptor 3 subunit) antibody
      • ITAM_HUMAN antibody
      • ITGAM antibody
      • Leukocyte adhesion receptor MO1 antibody
      • Ly-40 antibody
      • MAC 1 antibody
      • Mac-1a antibody
      • MAC1 antibody
      • Mac1, alpha subunit antibody
      • MAC1A antibody
      • Macrophage antigen alpha polypeptide antibody
      • MGC117044 antibody
      • Mo1 antibody
      • Mo1, alpha subunit antibody
      • MO1A antibody
      • Neutrophil adherence receptor alpha M subunit antibody
      • Neutrophil adherence receptor antibody
      • SLEB6 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human tonsil tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Flow Cytometry - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Flow Cytometry - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Flow cytometric analysis of TF-1 (human bone marrow erythroleukemia cell line) cell line labeling CD11b with ab224805 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue) .

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human colon tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human spleen tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human lung tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human cerebellum tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human thymus tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human lymph node tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human HK lymphoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

      Formalin-fixed, paraffin-embedded human endometrial adenocarcinoma tissue stained for CD11b using ab224805 at 1/100 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, and sodium azide (ab224805).

    • Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)
      Anti-CD11b antibody [SP330] - BSA and Azide free (ab241408)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab241408? Please let us know so that we can cite the reference in this datasheet.

    ab241408 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com