Recombinant Anti-CD13 antibody [WM15] (ab7417)

Thank you for your reply.
I cannot recommend this antibody for your purposes. Rat still shows a 77% homology and I am afraid that this could be boarder line and this antibody might cross-react.
It is always very difficult to positively proof that an antibody does not react with a certain species. If we do know this we will mention this on our datasheets in form of a "does not react" note. This indicated that the antibody was experimentally tested not to cross-react.
I attached a list of all our antibodies that react with human but do not react with rat for your information.
Maybe this list will include a suitable antibody for your purposes?
Please feel free to contact me again with any further questions.

Thank you for your inquiry.
This antibody is tested and guaranteed to work with human samples. Other species were not experimentally tested by us and we simply do not know i this antibody will cross-react.
Human CD13 show a high homology with CD13 of Gorilla and of non human primates (greater than 85%) and therefore it is very likely that this antibody will cross-react. I attached a overview to this email for your information.
If you are interested in using ab7417 with a species that shows 85% or more homology you might be eligible for our testing discount program.
I hope this information is helpful.
Please do not hesitate to contact me again with any further questions about ab7417 or the testing discount program.

DISCOUNT CODE: **********
Expiration date: *************

I am very pleased to hear you would like to accept our offer and test ab7417 in bovine samples. This code will give you 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for bovine samples and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you very much for your interest in ab7417.

To our knowledge, ab7417 has not been tested in on bovine samples, but based on sequence homology (there is a 87% level of homology between human and bovine), there is a high chance that this antibody will work for you.

Therefore, I can offer a discount off a future purchase if you buy ab7417 now, test it inbovine samplesand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab7417 inbovine samples. I will then send a discount code. This code must be issued before purchasing ab7417 so please wait for my reply before ordering.

2. Purchase ab7417 either by phone, fax, or online (www.abcam.com).

3. Test it inbovine samples.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab7417 turns out to be unsuitable forbovine samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for your enquiry. The best choice for a lysate would be a membrane fraction lysate, not a nuclear lysate, since CD13 is a trans-membrane protein. The antigen is expressed on a variety of tissues. The best choice is ab7913, THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate: https://www.abcam.com/THP1-whole-cell-lysate-ab7913.html We do not have a membrane fraction lysate for this cell line but this preparation will serve as well. Other tissue lysates that could work as positive controls are suggested by the SwissProt entry (accession number P15144) for CD13: "TISSUE SPECIFICITY: Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients." I hope this helps. Please do not hesitate to contact us if you have any other questions.