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  1. Link

    products/primary-antibodies/cd13-antibody-wm15-ab7417.pdf

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Immunology Cell Type Markers CD Myeloid Cells
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Validated using a knockout cell lineRecombinant

Recombinant Anti-CD13 antibody [WM15] (ab7417)

  • Datasheet
  • SDS
Reviews (7)Q&A (6)References (19)

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Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)
  • Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [WM15] to CD13
  • Suitable for: Flow Cyt, ICC/IF
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-CD13 antibody [WM15]
    See all CD13 primary antibodies
  • Description

    Mouse monoclonal [WM15] to CD13
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to Human CD13. Human AML cells

  • Epitope

    The antibody recognizes an extracellular epitope.
  • Positive control

    • ICC: THP-1, PANC-1 and A375 cells. Flow Cyt: Human peripheral blood leukocytes; Human Fibrosarcoma cells.
  • General notes

    This product has switched fom a hybridoma to recombinant production method on 08th March 2021.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Purification notes

    Purified by protein-A affinity chromatography > 95% pure (by SDS-PAGE).
  • Clonality

    Monoclonal
  • Clone number

    WM15
  • Myeloma

    NS1
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Microbiology
    • Organism
    • Virus
    • RNA Virus
    • ssRNA positive strand virus
    • SARS Coronavirus
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • B Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage

Associated products

  • Alternative Versions

    • Biotin Anti-CD13 antibody [WM15] (ab130430)
    • PerCP/Cy5.5® Anti-CD13 antibody [WM15] (ab157316)
    • Alexa Fluor® 488 Anti-CD13 antibody [WM15] (ab239271)
    • APC Anti-CD13 antibody [WM15] (ab239295)
    • PE/DyLight™ 594 Anti-CD13 antibody [WM15] (ab239330)
    • Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
    • FITC Anti-CD13 antibody [WM15] (ab52461)
    • PE Anti-CD13 antibody [WM15], prediluted (ab69775)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • KO cell lines

    • Human ANPEP (CD13) knockout THP-1 cell line (ab273759)
  • Recombinant Protein

    • Native human CD13 protein (ab134454)
  • Related Products

    • Human ANPEP (CD13) knockout THP-1 cell line (ab273759)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab7417 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (2)
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF (1)
Use a concentration of 1 µg/ml.

This product also produced a positive signal in A375 when fixed with 4% formaldehyde (10min)

Notes
Flow Cyt
Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF
Use a concentration of 1 µg/ml.

This product also produced a positive signal in A375 when fixed with 4% formaldehyde (10min)

Target

  • Function

    Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection.
  • Tissue specificity

    Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients.
  • Sequence similarities

    Belongs to the peptidase M1 family.
  • Domain

    Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies).
  • Post-translational
    modifications

    Sulfated.
    N- and O-glycosylated.
    May undergo proteolysis and give rise to a soluble form.
  • Cellular localization

    Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected.
  • Target information above from: UniProt accession P15144 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 290 Human
    • Omim: 151530 Human
    • SwissProt: P15144 Human
    • Unigene: 1239 Human
    • Alternative names

      • Alanyl (membrane) aminopeptidase antibody
      • Alanyl aminopeptidase antibody
      • Aminopeptidase M antibody
      • Aminopeptidase N antibody
      • AMPN_HUMAN antibody
      • ANPEP antibody
      • AP M antibody
      • AP N antibody
      • AP-M antibody
      • AP-N antibody
      • APN antibody
      • CD 13 antibody
      • CD13 antibody
      • CD13 antigen antibody
      • gp150 antibody
      • hAPN antibody
      • LAP 1 antibody
      • LAP1 antibody
      • Microsomal aminopeptidase antibody
      • Myeloid plasma membrane glycoprotein CD13 antibody
      • p150 antibody
      • PEPN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)
      Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

      ab7417 staining CD13 in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7417 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®; 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

      Also suitable in cells fixed with 4% paraformaldehyde (10 min).

      Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown

    • Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)
      Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)This image is courtesy of an anonymous Abreview

      This image was generated from the hybridoma version of the product.

      Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).

      See Abreview

    • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)
      Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)

      ab7417 staining CD13 in PANC-1 cells (top panel - positive control) and HEK-293 cells (bottom panel - negative control). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7417 at 0.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
      Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)
      Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)This image is courtesy of an anonymous Abreview

      This image was generated from the hybridoma version of the product.

      Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (19)

    Publishing research using ab7417? Please let us know so that we can cite the reference in this datasheet.

    ab7417 has been referenced in 19 publications.

    • Al-Mansoori L  et al. In vitro studies on CNGRC-CPG2 fusion proteins for ligand-directed enzyme prodrug therapy for targeted cancer therapy. Oncotarget 11:619-633 (2020). PubMed: 32110281
    • Xi Y  et al. Quantitative profiling of CD13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment. Nanoscale 11:1737-1744 (2019). PubMed: 30623954
    • Xu J  et al. SMAD4 Is Essential for Human Cardiac Mesodermal Precursor Cell Formation. Stem Cells 37:216-225 (2019). PubMed: 30376214
    • Donnenberg VS  et al. Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines. Cytometry A 93:448-457 (2018). PubMed: 29498809
    • Chen X  et al. Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-?B pathway. Cell Death Dis 9:207 (2018). PubMed: 29434225
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunocytochemistry/ Immunofluorescence abreview for Anti-CD13 antibody [WM15]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HT1080 fibrosarcoma)
    Specification
    HT1080 fibrosarcoma
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 04 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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