Recombinant Anti-CD13 antibody [WM15] (ab7417)
![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)](https://www.abcam.com/ps/products/7/ab7417/Images/ab7417-464499-anti-cd13-wm13-antibody-immunocytochemistry-a375-human.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [WM15] to CD13
- Suitable for: Flow Cyt, ICC/IF
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD13 antibody [WM15]
See all CD13 primary antibodies -
Description
Mouse monoclonal [WM15] to CD13 -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD13. Human AML cells
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Epitope
The antibody recognizes an extracellular epitope. -
Positive control
- ICC: THP-1, PANC-1 and A375 cells. Flow Cyt: Human peripheral blood leukocytes; Human Fibrosarcoma cells.
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General notes
This product has switched fom a hybridoma to recombinant production method on 08th March 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
Purified by protein-A affinity chromatography > 95% pure (by SDS-PAGE). -
Clonality
Monoclonal -
Clone number
WM15 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
- Biotin Anti-CD13 antibody [WM15] (ab130430)
- PerCP/Cy5.5® Anti-CD13 antibody [WM15] (ab157316)
- Alexa Fluor® 488 Anti-CD13 antibody [WM15] (ab239271)
- APC Anti-CD13 antibody [WM15] (ab239295)
- PE/DyLight™ 594 Anti-CD13 antibody [WM15] (ab239330)
- Anti-CD13 antibody [WM15] - BSA and Azide free (ab252261)
- FITC Anti-CD13 antibody [WM15] (ab52461)
- PE Anti-CD13 antibody [WM15], prediluted (ab69775)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7417 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | (2) |
Use a concentration of 1 µg/ml.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| ICC/IF | (1) |
Use a concentration of 1 µg/ml.
This product also produced a positive signal in A375 when fixed with 4% formaldehyde (10min) |
| Notes |
|---|
|
Flow Cyt
Use a concentration of 1 µg/ml. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 1 µg/ml. This product also produced a positive signal in A375 when fixed with 4% formaldehyde (10min) |
Target
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Function
Broad specificity aminopeptidase. Plays a role in the final digestion of peptides generated from hydrolysis of proteins by gastric and pancreatic proteases. May play a critical role in the pathogenesis of cholesterol gallstone disease. May be involved in the metabolism of regulatory peptides of diverse cell types including small intestinal and tubular epithelial cells, macrophages, granulocytes and synaptic membranes from the CNS. Found to cleave antigen peptides bound to major histocompatibility complex class II molecules of presenting cells and to degrade neurotransmitters at synaptic junctions. Is also implicated as a regulator of IL-8 bioavailability in the endometrium, and therefore may contribute to the regulation of angiogenesis. Is used as a marker for acute myeloid leukemia and plays a role in tumor invasion. In case of human coronavirus 229E (HCoV-229E) infection, serves as receptor for HCoV-229E spike glycoprotein. Mediates as well human cytomegalovirus (HCMV) infection. -
Tissue specificity
Expressed in epithelial cells of the kidney, intestine, and respiratory tract; granulocytes, monocytes, fibroblasts, endothelial cells, cerebral pericytes at the blood-brain barrier, synaptic membranes of cells in the CNS. Also expressed in endometrial stromal cells, but not in the endometrial glandular cells. Found in the vasculature of tissues that undergo angiogenesis and in malignant gliomas and lymph node metastases from multiple tumor types but not in blood vessels of normal tissues. A soluble form has been found in plasma. It is found to be elevated in plasma and effusions of cancer patients. -
Sequence similarities
Belongs to the peptidase M1 family. -
Domain
Amino acids 260-353 are essential to mediate susceptibility to infection with HCoV-229E (in porcine/human chimeric studies) and more specifically amino acids 288-295 (mutagenesis studies). -
Post-translational
modificationsSulfated.
N- and O-glycosylated.
May undergo proteolysis and give rise to a soluble form. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. A soluble form has also been detected. - Information by UniProt
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Database links
- Entrez Gene: 290 Human
- Omim: 151530 Human
- SwissProt: P15144 Human
- Unigene: 1239 Human
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Alternative names
- Alanyl (membrane) aminopeptidase antibody
- Alanyl aminopeptidase antibody
- Aminopeptidase M antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)
ab7417 staining CD13 in A375 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab7417 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor®; 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown
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![Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)](https://www.abcam.com/ps/products/7/ab7417/Images/ab7417-305145-anti-cd13-antibody-wm15-flow-cytometry.jpg)
Flow Cytometry - Anti-CD13 antibody [WM15] (ab7417)This image is courtesy of an anonymous AbreviewThis image was generated from the hybridoma version of the product.
Ab7417 staining CD13 in Human Fibrosarcoma cells by flow cytometry. Cells were fixed with paraformaldehyde. The sample was incubated with primary antibody at 10µg/ml in PBS for 1 hour at 20°C. An Allophycocyanin (APC) conjugated anti-mouse monoclonal was used as a secondary antibody at 5µg/ml. Secondary antibody only (red). Ab7417 and secondary antibody (blue).
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![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)](https://www.abcam.com/ps/products/7/ab7417/Images/ab7417-9-anti-CD13-antibody-WM15-immunocytochemistry-immunofluorescence-panc1-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)ab7417 staining CD13 in PANC-1 cells (top panel - positive control) and HEK-293 cells (bottom panel - negative control). The cells were fixed with 4% paraformaldehyde (10 min) then permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7417 at 0.5μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
![Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)](https://www.abcam.com/ps/products/7/ab7417/Images/ab7417-305119-anti-cd13-antibody-wm15-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD13 antibody [WM15] (ab7417)This image is courtesy of an anonymous AbreviewThis image was generated from the hybridoma version of the product.
Ab7417 staining CD13 in HT1080 cells by ICC/IF (Immunocytochemistry/Immunofluorescence). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at 10µg/ml for 1 hour. A Texas Red Goat Anti-mouse polyclonal was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab7417 has been referenced in 19 publications.
- Al-Mansoori L et al. In vitro studies on CNGRC-CPG2 fusion proteins for ligand-directed enzyme prodrug therapy for targeted cancer therapy. Oncotarget 11:619-633 (2020). PubMed: 32110281
- Xi Y et al. Quantitative profiling of CD13 on single acute myeloid leukemia cells by super-resolution imaging and its implication in targeted drug susceptibility assessment. Nanoscale 11:1737-1744 (2019). PubMed: 30623954
- Xu J et al. SMAD4 Is Essential for Human Cardiac Mesodermal Precursor Cell Formation. Stem Cells 37:216-225 (2019). PubMed: 30376214
- Donnenberg VS et al. Antibody-based cell-surface proteome profiling of metastatic breast cancer primary explants and cell lines. Cytometry A 93:448-457 (2018). PubMed: 29498809
- Chen X et al. Mechanical stretch-induced osteogenic differentiation of human jaw bone marrow mesenchymal stem cells (hJBMMSCs) via inhibition of the NF-?B pathway. Cell Death Dis 9:207 (2018). PubMed: 29434225