Recombinant Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)
![Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)](https://www.abcam.com/ps/products/283/ab283569/Images/ab283569-1-anti-cd130-gp130-antibody-epr245575-flow-cytometry-daudi.jpg "Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR24557-5] to CD130 (gp130)
- Suitable for: Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD130 (gp130) antibody [EPR24557-5]
See all CD130 (gp130) primary antibodies -
Description
Rabbit monoclonal [EPR24557-5] to CD130 (gp130) -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P,IP or WB -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Flow Cyt: MG63, Wild-type A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR24557-5 -
Isotype
IgG -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab283569 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50.
|
| Notes |
|---|
|
Flow Cyt
1/50. |
Target
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Function
Signal-transducing molecule. The receptor systems for IL6, LIF, OSM, CNTF, IL11, CTF1 and BSF3 can utilize gp130 for initiating signal transmission. Binds to IL6/IL6R (alpha chain) complex, resulting in the formation of high-affinity IL6 binding sites, and transduces the signal. Does not bind IL6. May have a role in embryonic development (By similarity). The type I OSM receptor is capable of transducing OSM-specific signaling events. -
Tissue specificity
Found in all the tissues and cell lines examined. Expression not restricted to IL6 responsive cells. -
Sequence similarities
Belongs to the type I cytokine receptor family. Type 2 subfamily.
Contains 5 fibronectin type-III domains.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain. -
Domain
The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation. -
Post-translational
modificationsPhosphorylation of Ser-782 down-regulates cell surface expression.
Heavily N-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3572 Human
- Omim: 600694 Human
- SwissProt: P40189 Human
- Unigene: 532082 Human
- Unigene: 706627 Human
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Alternative names
- CD130 antibody
- CD130 antigen antibody
- CDw130 antibody
see all
Images
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Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)
Flow cytometric analysis of Daudi (Human Burkitt's lymphoma lymphoblast) (Left) / MG-63 (human osteosarcoma fibroblast) (Right) cells labelling CD130 (gp130) with ab283569 at 1/50 dilution (1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
Low expression: Daudi (PMID: 2261637).
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![Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)](https://www.abcam.com/ps/products/283/ab283569/Images/ab283569-2-anti-cd130-gp130-antibody-epr245575-flow-cytometry-wildtype-a549.jpg)
Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)Flow cytometric analysis of Wild-type A549 (Human lung carcinoma epithelial cell) (Right) / CD130 (gp130) knockout A549 cells (Left) cells labelling CD130 (gp130) with ab283569 at 1/500 dilution (0.1ug)/ (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat F(ab')2 Anti-Rabbit IgG(DyLight® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.
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![Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)](https://www.abcam.com/ps/products/283/ab283569/Images/ab283569-480309-anti-CD130-YCAR245575-HT-H1L1-Human-Human-PBMCs-cells-flow-cytometry.jpg)
Flow Cytometry - Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab283569 (right) or Rabbit IgG (monoclonal) (ab172730) isotype (left). PBMCs were incubated for 30 min on ice in 1x PBS containing 10µg/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody (ab283569) or Rabbit IgG (monoclonal) (ab172730) isotype (1x106 in 100µl; with 0.01µg antibody) for 30 min on ice.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1:2000 dilution for 30 min on ice.
The cells were simultaneously stained with CD14 PE.
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.
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![Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)](https://www.abcam.com/ps/products/283/ab283569/Images/ab283569-3-Benefits-of-Recombinant-antibodies.jpg)
Anti-CD130 (gp130) antibody [EPR24557-5] (ab283569)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab283569 has not yet been referenced specifically in any publications.