Recombinant Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-326959-anti-cd134-antibody-epr22229-5-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22229-5] to CD134 / OX40L receptor
- Suitable for: WB, IHC-P, Flow Cyt, IP
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-CD134 / OX40L receptor antibody [EPR22229-5]
See all CD134 / OX40L receptor primary antibodies -
Description
Rabbit monoclonal [EPR22229-5] to CD134 / OX40L receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cyt, IPmore details
Unsuitable for: ICC/IF or IHC-Fr -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: Mouse and rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysates. IHC-P: Mouse and rat spleen tissues. Flow Cyt: Rat and mouse primary splenocytes treated with 2.5µg/ml concanavalin A for 72h. IP: Rat splenocytes treated with 2.5µg/ml concanavalin A for 72h lysate.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22229-5 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229021 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 29 kDa).
|
|
| IHC-P | (1) |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Flow Cyt |
1/500.
|
|
| IP |
1/30.
|
| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
Flow Cyt
1/500. |
|
IP
1/30. |
Target
-
Function
Receptor for TNFSF4/OX40L/GP34. -
Sequence similarities
Contains 4 TNFR-Cys repeats. -
Cellular localization
Membrane. - Information by UniProt
-
Database links
- Entrez Gene: 22163 Mouse
- Entrez Gene: 25572 Rat
- SwissProt: P47741 Mouse
- SwissProt: P15725 Rat
- Unigene: 13885 Mouse
- Unigene: 48883 Rat
-
Alternative names
- ACT 35 antibody
- ACT35 antibody
- ACT35 antigen antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in rat spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.
The section was incubated with ab229021 for 10 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-326958-anti-cd134-antibody-epr22229-5-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD134 / OX40L using ab229021 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining on T cells in periarterial lymphatic sheath of mouse spleen (PMID: 15259024; PMID: 10233901). Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10mins.
The section was incubated with ab229021 for 10 mins at 37℃. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.
-
![Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-326957-anti-cd134-antibody-epr22229-5-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)CD134 / OX40L was immunoprecipitated from 0.35 mg rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate using ab229021 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab229021 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at a 1/5000 dilution.
Lane 1: Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate 10 μg (input)
Lane 2: ab229021 IP in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab229021 in Rat splenocytes treated with 2.5μg/ml concanavalin A for 72h lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 20 seconds. -
![Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-326956-anti-cd134-antibody-epr22229-5-flow-cytometry.jpg)
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)Flow cytometric analysis of mouse primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/2000 dilution.
Data shown were gated on viable cells.
-
![Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-326955-anti-cd134-antibody-epr22229-5-flow-cytometry.jpg)
Flow Cytometry - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)Flow cytometric analysis of rat primary splenocytes treated with 2.5μg/ml concanavalin A for 72h labeling CD134 / OX40L with ab229021 at 1/500 dilution (Right) compared to cells stained with Alexa Fluor® 647-conjugated CD3 and rabbit IgG (Left). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at 1/2000 dilution.
Data shown were gated on viable cells.
-
![Western blot - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-326954-anti-cd134-antibody-epr22229-5-western-blot.jpg)
Western blot - Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)All lanes : Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021) at 1/1000 dilution
Lane 1 : Untreated mouse splenocytes lysate
Lane 2 : Mouse splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate
Lane 3 : Untreated rat splenocytes lysate
Lane 4 : Rat splenocytes treated with 2.5ug/ml concanavalin A for 72h lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 29 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Diluting buffer and concentration: 5% NFDM/TBST
CD134 / OX40L is a glycosylated protein and its expression is increased after concanavalin A treatment. PMID 23897980; PMID 17609274
-
![Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)](https://www.abcam.com/ps/products/229/ab229021/Images/ab229021-7-benefits-of-recombinant-antibodies.png)
Anti-CD134 / OX40L receptor antibody [EPR22229-5] (ab229021)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (0)
ab229021 has not yet been referenced specifically in any publications.