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Anti-CD14 antibody [4B4F12] (ab182032)

Reviews (1) Submit a question References (28)
Flow Cytometry - Anti-CD14 antibody [4B4F12] (ab182032)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)
  • Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)
  • Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
  • Western blot - Anti-CD14 antibody [4B4F12] (ab182032)

Key features and details

  • Mouse monoclonal [4B4F12] to CD14
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-CD14 antibody [4B4F12]
    See all CD14 primary antibodies

  • Description

    Mouse monoclonal [4B4F12] to CD14

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment corresponding to Human CD14 aa 1-250. Expressed in E. coli.
    Database link: P08571

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • Human CD14 recombinant protein (amino acids 20-214), lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc, HepG2, A549, HL60, mouse RAW264.7, Hela, HEK293 and mouse NIH 3T3 cell lysates. Jurkat cells. Human stomach cancer tissue.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-P (1)
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Notes
WB
1/500 - 1/2000. Predicted molecular weight: 40 kDa.
IHC-P
1/200 - 1/1000.
Flow Cyt
1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

  • Tissue specificity

    Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

  • Sequence similarities

    Contains 11 LRR (leucine-rich) repeats.

  • Post-translational
    modifications

    N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 14 antibody
      • CD_antigen=CD14 antibody
      • CD14 antibody
      • CD14 antigen antibody
      • CD14 molecule antibody
      • CD14_HUMAN antibody
      • LPS-R antibody
      • Mo2 antibody
      • Monocyte differentiation antigen CD14 antibody
      • Monocyte differentiation antigen CD14 urinary form antibody
      • Monocyte differentiation antigen CD14, membrane-bound form antibody
      • Myeloid cell specific leucine rich glycoprotein antibody
      • Myeloid cell-specific leucine-rich glycoprotein antibody
      see all

    Images

    • Flow Cytometry - Anti-CD14 antibody [4B4F12] (ab182032)
      Flow Cytometry - Anti-CD14 antibody [4B4F12] (ab182032)

      Flow cytometric analysis of Jurkat cells labeling CD14 using ab182032 at 1/200 (green) and a negative control (red).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)

      Immunohistochemical analysis of paraffin-embedded, DAB-stained Human stomach cancer tissues labeling CD14 using ab182032 at 1/200.

    • Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
      Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
      Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution + Human CD14 recombinant protein (amino acids 20-214)

      Predicted band size: 40 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [4B4F12] (ab182032)This image is courtesy of an anonymous abreview.

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue section labeling CD14 with ab182032. The tissue was fixed with formaldehyde, heat mediated antigen retrieval was performed followed by blocking with 5% serum for 12 hours at 4°C. The tissue was incubated with undiluted Anti-CD14 antibody [4B4F12] (ab182032) for 12 hours at 4°C.

      See Abreview

    • Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
      Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
      All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution

      Lane 1 : Lysate from HEK293 cells
      Lane 2 : Lysate from HEK293 cells transfected with CD14 (amino acids 20-214)-hIgGFc

      Predicted band size: 40 kDa

    • Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
      Western blot - Anti-CD14 antibody [4B4F12] (ab182032)
      All lanes : Anti-CD14 antibody [4B4F12] (ab182032) at 1/500 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HL60 cell lysate
      Lane 4 : mouse RAW264.7 cell lysate
      Lane 5 : HeLa cell lysate
      Lane 6 : HEK293 cell lysate
      Lane 7 : mouse NIH 3T3 cell lysate

      Predicted band size: 40 kDa

    References (28)

    Publishing research using ab182032? Please let us know so that we can cite the reference in this datasheet.

    ab182032 has been referenced in 28 publications.

    • Guo D  et al. ELK1-Induced upregulation of long non-coding TNK2-AS1 promotes the progression of acute myeloid leukemia by EZH2-mediated epigenetic silencing of CELF2. Cell Cycle 22:117-130 (2023). PubMed: 35941836
    • Mo YQ  et al. Lysosomal exocytosis of HSP70 stimulates monocytic BMP6 expression in Sjögren's syndrome. J Clin Invest 132:N/A (2022). PubMed: 35113815
    • Nakamura A  et al. The potential of soluble CD14 in discriminating nonalcoholic steatohepatitis from nonalcoholic fatty liver disease. Hepatol Res 52:508-521 (2022). PubMed: 35129841
    • Wu Z  et al. sGRP78 enhances selective autophagy of monomeric TLR4 to regulate myeloid cell death. Cell Death Dis 13:587 (2022). PubMed: 35798718
    • Zadka Ł  et al. Interplay of stromal tumor-infiltrating lymphocytes, normal colonic mucosa, cancer-associated fibroblasts, clinicopathological data and the immunoregulatory molecules of patients diagnosed with colorectal cancer. Cancer Immunol Immunother 70:2681-2700 (2021). PubMed: 33625532
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD14 [4B4F12] antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Antigen retrieval step
    Heat mediated
    Sample
    Mouse Tissue sections (Liver (Kupffer cells))
    Specification
    Liver (Kupffer cells)
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Jul 01 2014

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