Name: Anti-CD14 antibody [SP192]
Brand: RabMAb
SKU: ab183322
Rating: 5 (5 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [SP192] to CD14
Suitable for: Flow Cyt, IHC-P, WB
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free PE

Product name

Anti-CD14 antibody [SP192]
See all CD14 primary antibodies
Description

Rabbit monoclonal [SP192] to CD14
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WBmore details
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-p: Human tonsil lysate; Human tonsil, Human thymus, Human colon, Human colon adenocarcinoma, Human spleen, Human lung, Human liver, Human esophagus, Human endometrium, Human HK lymphoma, Human B cell lymphoma and Human bladder tissues. Flow Cyt: PBMC
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
Purity

Protein A/G purified
Purification notes

Purified from TCS by protein A/G.
Clonality

Monoclonal
Clone number

SP192
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD14 antibody [SP192] - BSA and Azide free (ab230903)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Mouse CD14 protein (ab207103)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab183322 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		1/200.
IHC-P	(2)	1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
WB		1/100. Predicted molecular weight: 40 kDa.

Notes
Flow Cyt 1/200.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at room temperature for 20 min.
WB 1/100. Predicted molecular weight: 40 kDa.

Function

Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.
Tissue specificity

Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
Sequence similarities

Contains 11 LRR (leucine-rich) repeats.
Post-translational
modifications

N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Cellular localization

Cell membrane.
Target information above from: UniProt accession P08571 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 929 Human
- Omim: 158120 Human
- SwissProt: P08571 Human
- Unigene: 163867 Human
Alternative names
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
- CD14 antigen antibody
- CD14 molecule antibody
- CD14_HUMAN antibody
- LPS-R antibody
- Mo2 antibody
- Monocyte differentiation antigen CD14 antibody
- Monocyte differentiation antigen CD14 urinary form antibody
- Monocyte differentiation antigen CD14, membrane-bound form antibody
- Myeloid cell specific leucine rich glycoprotein antibody
- Myeloid cell-specific leucine-rich glycoprotein antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on stromal cells in the human colon, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183322 for 10 mins at room temperature.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD14 with ab183322 at 1/100 dilution (1.61 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab183322 for 10 mins at room temperature.
Flow Cytometry - Anti-CD14 antibody [SP192] (ab183322)

Flow Cytometry analysis of human PBMC (human peripheral blood mononuclear cell) cells labeling CD14 with purified ab183322 at 1:200 dilution (0.805 µg/ml) - Red. A Goat anti rabbit IgG (Dylight® 488, ab98462) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human thymus tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human bone marrow tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human colon tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human colon adenocarcinoma tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human spleen tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human lung tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human esophagus tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human endometrium tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human HK lymphoma tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human B cell lymphoma tissue labeling CD14 with ab183322 at 1/100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD14 antibody [SP192] (ab183322)

Immunohistochemical analysis of paraffin embedded Human bladder tissue labeling CD14 with ab183322 at 1/100.
Western blot - Anti-CD14 antibody [SP192] (ab183322)

Anti-CD14 antibody [SP192] (ab183322) at 1/100 dilution + Human tonsil lysate

Predicted band size: 40 kDa
Anti-CD14 antibody [SP192] (ab183322)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab183322? Please let us know so that we can cite the reference in this datasheet.

ab183322 has been referenced in 10 publications.

Yang S et al. Lnc RNA ZFAS1 regulates the proliferation, apoptosis, inflammatory response and autophagy of fibroblast-like synoviocytes via miR-2682-5p/ADAMTS9 axis in rheumatoid arthritis. Biosci Rep 40:N/A (2020). PubMed: 32744323
Belbellaa B et al. High Levels of Frataxin Overexpression Lead to Mitochondrial and Cardiac Toxicity in Mouse Models. Mol Ther Methods Clin Dev 19:120-138 (2020). PubMed: 33209958
Lu Q et al. Hypoxic Tumor-Derived Exosomal Circ0048117 Facilitates M2 Macrophage Polarization Acting as miR-140 Sponge in Esophageal Squamous Cell Carcinoma. Onco Targets Ther 13:11883-11897 (2020). PubMed: 33239890
Kudo SE et al. Depressed Colorectal Cancer: A New Paradigm in Early Colorectal Cancer. Clin Transl Gastroenterol 11:e00269 (2020). PubMed: 33512809
Gomez-Lopez N et al. The origin of amniotic fluid monocytes/macrophages in women with intra-amniotic inflammation or infection. J Perinat Med 47:822-840 (2019). PubMed: 31494640
Escudero-Pérez B et al. Comparative pathogenesis of Ebola virus and Reston virus infection in humanized mice. JCI Insight 4:N/A (2019). PubMed: 31550241
Pu X et al. Silencing stomatin-like protein 2 attenuates tumor progression and inflammatory response through repressing CD14 in liver cancer. Onco Targets Ther 12:7361-7373 (2019). PubMed: 31571899
Bajpai G et al. The human heart contains distinct macrophage subsets with divergent origins and functions. Nat Med 24:1234-1245 (2018). PubMed: 29892064
Takahashi Y et al. Monocyte chemoattractant protein 1 expression and proliferation in primary central nervous system lymphoma. Oncol Lett 14:264-270 (2017). PubMed: 28693163
Hamada D et al. Suppressive Effects of Insulin on Tumor Necrosis Factor-Dependent Early Osteoarthritic Changes Associated With Obesity and Type 2 Diabetes Mellitus. Arthritis Rheumatol 68:1392-402 (2016). PubMed: 26713606

Customer reviews and Q&As

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1-5 of 5 Abreviews or Q&A

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (Tonsil)

Permeabilization

Specification

Tonsil

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3%

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Nov 24 2021

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Rhesus monkey Tissue sections (colon)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate pH 6

Permeabilization

Specification

colon

Blocking step

Serum as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 23°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 18 2019

Application

Immunohistochemistry free floating

Sample

Human Tissue sections (Human inflammed lung tissue)

Specification

Human inflammed lung tissue

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. James Xiao

Verified customer

Submitted Jan 06 2018

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Colorectal)

Antigen retrieval step

Heat mediated

Specification

Colorectal

Blocking step

(agent) for 20 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

MR. Christopher Bagnall

Verified customer

Submitted Sep 13 2017

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (Colon)

Specification

Colon

Blocking step

H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Sep 08 2017

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Purification notes

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Post-translationalmodifications

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (10)

Customer reviews and Q&As

Immunohistochemistry (Frozen sections) abreview for Anti-CD14 antibody [SP192]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD14 antibody [SP192]

Immunohistochemistry free floating abreview for Anti-CD14 antibody [SP192]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD14 antibody [SP192]

Immunocytochemistry/ Immunofluorescence abreview for Anti-CD14 antibody [SP192]

Post-translational
modifications