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Anti-CD14 antibody [UCHM1] (ab6623)

Submit a review Q&A (1)
Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)

    Key features and details

    • Mouse monoclonal [UCHM1] to CD14
    • Suitable for: Flow Cyt
    • Reacts with: Human
    • Isotype: IgG2a

    Overview

    • Product name

      Anti-CD14 antibody [UCHM1]
      See all CD14 primary antibodies

    • Description

      Mouse monoclonal [UCHM1] to CD14

    • Host species

      Mouse

    • Specificity

      ab6623 recognises a monocyte surface antigen of 55 kDa found chiefly on monocytes. UCHM1 was clustered at the Third International Workshop and Conference on Human Leucocyte Differentiation Antigens. Oxford 1986, as CDw14, group A. In tonsil tissue sections UCHM1 gives positive staining reactions with monocytic cells, the interfollicular tissue macrophages seen under the capsule, and dendritic reticulum cells. Skin Langerhans cells are always negative. UCHM1 also reacts with Kupffer cells and sinus lining cells on the liver.

    • Tested applications

      Suitable for: Flow Cytmore details
      Unsuitable for: IHC-P

    • Species reactivity

      Reacts with: Human

    • Immunogen

      Tissue, cells or virus corresponding to Human CD14. Human Thymocytes followed by peripheral blood mononuclear cells

    • Positive control

      • Tonsil.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab6623 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
    Notes
    Flow Cyt
    Use at an assay dependent concentration.

    ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 
    Application notes
    Is unsuitable for IHC-P.

    Target

    • Function

      Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.

    • Tissue specificity

      Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.

    • Sequence similarities

      Contains 11 LRR (leucine-rich) repeats.

    • Post-translational
      modifications

      N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

    • Database links

      • Alternative names

        • CD 14 antibody
        • CD_antigen=CD14 antibody
        • CD14 antibody
        • CD14 antigen antibody
        • CD14 molecule antibody
        • CD14_HUMAN antibody
        • LPS-R antibody
        • Mo2 antibody
        • Monocyte differentiation antigen CD14 antibody
        • Monocyte differentiation antigen CD14 urinary form antibody
        • Monocyte differentiation antigen CD14, membrane-bound form antibody
        • Myeloid cell specific leucine rich glycoprotein antibody
        • Myeloid cell-specific leucine-rich glycoprotein antibody
        see all

      Images

      • Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)
        Flow Cytometry - Anti-CD14 antibody [UCHM1] (ab6623)
        ab6623 staining of human peripheral blood monocytes, visualised with rabbit anti-mouse IgG: FITC.

      References (0)

      Publishing research using ab6623? Please let us know so that we can cite the reference in this datasheet.

      ab6623 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Can this antibody be used with paraffin sections or only frozen as stated on the datasheet?

      Read More
      Answer

      This antibody has been tested on paraffin sections and does not work. In IHC, it is known to work only with frozen sections.

      Read More

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