Recombinant Anti-CD147 antibody [EPR4053] (ab108308)

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. It does look like ab108308 may be the best fit for your experiment, so as requested, I have issued a free of charge replacement of ab108308.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Thank you for your reply.

I would be more than happy to send you a replacement antibody. If you could let me know the catalogue number of the antibody you would like as a replacement, I will be happy to arrange that for you.

As for ab666, we have only tested it on human samples and so not have any other data on how it would react with other species.

Looking forward to your reply.

Thank you for your message and for providing this further information.

I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments, and would like to offer a free of charge replacement in compensation (providing the product has been purchased in the last 120 days). In order to arrange this, I would appreciate if you could confirm your order number and date of purchase?

Thank you for your continued cooperation. I look forward to hearing from you with details of how you would like to proceed.

Thank you for contacting us.


I am sorry to hear that of this problem. No or low signal can be the result of a number of factors. As this product is a tissue culture supernatant I would suggest that prehaps increasing the antibody concentration may help. If you could send me the protocol that you have used I may be able to offer furhter tips as well.



This product is covered by our Abpromise guarantee.If we cannot remedy this issue and this is a product that you have purchased within the last six months, we will replace or refund it under our Abpromise guarantee, if being used according to specifications listed on our datasheet.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Thank you for your reply and for kindly confirming these details.

I am sorry that this antibody did not perform as stated on the datasheet. As requested, I have asked our Finance department to issue a credit note for you.

Credit ID: ####

The credit note may be used in one of the following ways:

(1) Redeemed against the original invoice if this hasn't already been paid. (2) Held on the account for use against a future order. (3) A full refund can be offered where no other invoices are outstanding.

Please contact your Finance department to confirm how you would like the credit note to be used and ensure it is not redeemed without your knowledge.

To specifically receive a refund please ask your Finance department to contact our Finance department at creditcontrol@abcam.com or by telephone using the information at the “Contact Us” link in the top right corner of our website.

The credit note ID is for your reference only, please refer to the credit note ID in any correspondence with our accounting department. We will send you the completed credit note by email or postal mail with the actual credit note number which will start with the letters CGB.

In addition to this, having reviewed the details provided, I recommend you may like to consider the following for future experiments, which I hope would be helpful:

1. How long was the fixation? We would recommend 10 minutes only to ensure there is not too much crosslinking.

2. As CD147 is a cell membrane protein, permeabilization is not required. Using a strong detergent like Triton can disrupt membrane proteins and significantly affect some epitopes which can affect the results of the staining. Epitopes for individual antibodies could be affected differently, which may be why the other antibody is working.

3. A more gentle detergent such as 0.1% Tween 20 can be used in the wash buffer and antibody dilution buffer to help keep the antibody solubilized.

4. Overnight incubation at 4oC with primary antibody can often provide more effective and specific staining.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service should you require further expert advice

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been succesful.

I would like to reassure you that this antibody is tested and covered by our guarantee for IHC-P, ICC and human samples. Before deciding how to proceed, I would like to investigate this particular case further for you, and also obtain some further information for our quality montoring.

In order to do this, I have enclosed a questionnaire below. I would appreciate if you could complete this. It will help you put the information we require together very easily. I have completed the sections for which information has already been provided.

In the event that a product is not functioning in the tested applications and species cited on the product data sheet (and the problem has been reported within 6 months of purchase), we will be pleased to provide a credit note, free of charge replacement or refund.

Thank you for your time and cooperation. We look forward to receiving the completed questionaire and details of how you would like to proceed.

Order Details

Antibody code:

Lot number:
Purchase order number
or preferably Abcam order number:

General Information

Antibody storage conditions (temperature/reconstitution etc)
Description of the problem (high background, low signal, non-specific satining etc.)

Sample (Species/Tissue/Cell Type/Cell Line etc.)

Fixation of sample (Ethanol/Methanol/Acetone/Paraformaldehyde/Other/Duration etc.)

Antigen retrieval (Enzymatic method, Heat mediated technique etc.)

Permeabilization step

Blocking conditions (Buffer/time period, Blocking agent etc.)

Primary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Secondary Antibody (Manufacturer/Species/Diluent/Dilution/Incubation time, Wash step)

Detection method

Positive and negative controls used (please specify)

Optimization attempts (problem solving)

How many times have you tried the IHC?

Have you run a "No Primary" control?
Yes No

Do you obtain the same results every time?
Yes No

What steps have you altered?

Additional Notes

We would appreciate if you are also able to provide and image which would help us to assess the results