For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/cd147-antibody-mem-m62-ab21903.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Immunology Cell Type Markers CD Endothelial Cells
Share by email
Validated using a knockout cell line

Anti-CD147 antibody [MEM-M6/2] (ab21903)

  • Datasheet
  • SDS
Reviews (1) Submit a question

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)

    Key features and details

    • Mouse monoclonal [MEM-M6/2] to CD147
    • Suitable for: Flow Cyt
    • Knockout validated
    • Reacts with: Human
    • Isotype: IgG2b

    You may also be interested in

    Knockout
    Product image
    Human BSG (CD147) knockout A549 cell line (ab273748)
    Primary
    PE Anti-CD147 antibody [MEM-M6/1] (ab77133)

    View more associated products

    Overview

    • Product name

      Anti-CD147 antibody [MEM-M6/2]
      See all CD147 primary antibodies
    • Description

      Mouse monoclonal [MEM-M6/2] to CD147
    • Host species

      Mouse
    • Tested applications

      Suitable for: Flow Cytmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      A soluble recombinant form of CD147, CD147Rg, which consists of the cDNA coding for the entire extracellular region of CD147 fused to the DNA coding for the hinge region, CH2 and CH3 domain of human IgG1.

    • Epitope

      This antibody recognises extracellular Ig domain D1 of CD147 antigen.
    • Positive control

      • Flow Cyt: A549 cells.
    • General notes

      This product was changed from ascites to tissue culture supernatant. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      pH: 7.40
      Preservative: 0.097% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A purified
    • Purification notes

      > 95% (by SDS-PAGE). Purified from TCS
    • Clonality

      Monoclonal
    • Clone number

      MEM-M6/2
    • Isotype

      IgG2b
    • Research areas

      • Immunology
      • Cell Type Markers
      • CD
      • Endothelial Cells
      • Neuroscience
      • Sensory System
      • Visual system
      • Neuroscience
      • Neurology process
      • Neurogenesis
      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus
      • Cancer
      • Invasion/microenvironment
      • ECM
      • Extracellular matrix
      • Other
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Types of disease
      • Cancer
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers
      • Neuroscience
      • Processes

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Isotype control

      • Mouse IgG2b, kappa monoclonal [7E10G10] - Isotype Control (ab170192)
    • KO cell lines

      • Human BSG (CD147) knockout A549 cell line (ab273748)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab21903 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Flow Cyt
    Use a concentration of 5 µg/ml.
    Notes
    Flow Cyt
    Use a concentration of 5 µg/ml.

    Target

    • Function

      Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.
    • Tissue specificity

      Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.
    • Sequence similarities

      Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
      Contains 1 Ig-like V-type (immunoglobulin-like) domain.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Target information above from: UniProt accession P35613 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 682 Human
      • Omim: 109480 Human
      • SwissProt: P35613 Human
      • Unigene: 501293 Human
      • Alternative names

        • 5A11 antigen antibody
        • 5F7 antibody
        • BASI_HUMAN antibody
        • Basigin (Ok blood group) antibody
        • Basigin antibody
        • Blood brain barrier HT7 antigen antibody
        • Bsg antibody
        • CD 147 antibody
        • CD147 antibody
        • CD147 antigen antibody
        • Collagenase stimulatory factor antibody
        • EMMPRIN antibody
        • Extracellular matrix metalloproteinase inducer antibody
        • Leukocyte activation antigen M6 antibody
        • M 6 antibody
        • M6 antibody
        • M6 leukocyte activation antigen antibody
        • Neurothelin antibody
        • OK antibody
        • OK blood group antibody
        • OK blood group antigen antibody
        • TCSF antibody
        • Tumor cell derived collagenase stimulatory factor antibody
        • Tumor cell-derived collagenase stimulatory factor antibody
        see all

      Images

      • Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)
        Flow Cytometry - Anti-CD147 antibody [MEM-M6/2] (ab21903)

        Flow cytometry overlay histogram showing wild-type A549 (green line) and BSG knockout A549 cells (ab273748) stained with ab21903 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab21903) (1x106 in 100μl at 5 μg/ml) for 30 min at 4°C.

        The secondary antibody Goat anti-mouse IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150117) was used at 1/2000 for 30 min at 4°C.

        Isotype control antibody was mouse IgG2bκ (ab170192) used at the same concentration and conditions as the primary antibody (wild-type A549 - black line; BSG knockout A549 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

        Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (0)

      Publishing research using ab21903? Please let us know so that we can cite the reference in this datasheet.

      ab21903 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Western blot abreview for Anti-CD147 antibody [MEM-M6/2]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Cell lysate - whole cell (brain endothelial cells)
      Gel Running Conditions
      Reduced Denaturing (4-12 %)
      Loading amount
      1.7 µg
      Specification
      brain endothelial cells
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      Submitted Mar 23 2017

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2023 Abcam plc. All rights reserved.