Anti-CD147 antibody [OTI9B10] (ab119020)
![Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)](https://www.abcam.com/ps/products/119/ab119020/Images/ab119020-446554-anti-cd147-antibody-oti9b10-western-blot-mps-a549-wt-mps-a549-cd147bsg-knockout-raji-jurkat.jpg "Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)")
Key features and details
- Mouse monoclonal [OTI9B10] to CD147
- Suitable for: WB, ICC/IF
- Knockout validated
- Reacts with: Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-CD147 antibody [OTI9B10]
See all CD147 primary antibodies -
Description
Mouse monoclonal [OTI9B10] to CD147 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt -
Species reactivity
Reacts with: Human, African green monkey -
Immunogen
Recombinant full length protein corresponding to Human CD147.
Database link: NP_001719 -
Positive control
- WB: A549, Raji and Jurkat cell lysates; HEK293T cell lysate transfected with pCMV6-ENTRY CD147 cDNA ICC/IF: COS7 cells transiently transfected with pCMV6-ENTRY CD147
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General notes
The clone number has been updated from 9B10 to OTI9B10, both clone numbers name the same clone.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI9B10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab119020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/2000. Predicted molecular weight: 42 kDa.
|
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| ICC/IF |
1/100.
|
| Notes |
|---|
|
WB
1/2000. Predicted molecular weight: 42 kDa. |
|
ICC/IF
1/100. |
Target
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Function
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). May target monocarboxylate transporters SLC16A1, SLC16A3 and SLC16A8 to plasma membranes of retinal pigment epithelium and neural retina. Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes. -
Tissue specificity
Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas. -
Sequence similarities
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. Melanosome. Colocalizes with SLC16A1 and SLC16A8 (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 682 Human
- Omim: 109480 Human
- SwissProt: P35613 Human
- Unigene: 501293 Human
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Alternative names
- 5A11 antigen antibody
- 5F7 antibody
- BASI_HUMAN antibody
see all
Images
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Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)All lanes : Anti-CD147 antibody [OTI9B10] (ab119020) at 1/2000 dilution
Lane 1 : Wild-type A549 cell lysate
Lane 2 : BSG knockout A549 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : Jurkat cell lysate
Lysates/proteins at 30 µg per lane.
Performed under reducing conditions.
Predicted band size: 42 kDa
Observed band size: 55-70 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab119020 observed at 55-70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab119020 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab119020 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (ab119020)](https://www.abcam.com/ps/products/119/ab119020/Images/CD147-Primary-antibodies-ab119020-2.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD147 antibody [OTI9B10] (ab119020)ab119020, at a 1/100 dilution, staining CD147 in COS7 cells transiently transfected with pCMV6-ENTRY CD147 by Immunofluorescence. -
![Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)](https://www.abcam.com/ps/products/119/ab119020/Images/CD147-Primary-antibodies-ab119020-1.jpg)
Western blot - Anti-CD147 antibody [OTI9B10] (ab119020)All lanes : Anti-CD147 antibody [OTI9B10] (ab119020) at 1/2000 dilution
Lane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY CD147 cDNA
Lysates/proteins at 5 µg per lane.
Predicted band size: 42 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab119020 has been referenced in 1 publication.
- Li X et al. Overexpression of monocarboxylate transporter 4 promotes the migration and invasion of non-carcinogenic L929 fibroblast cells. Oncol Lett 21:44 (2021). PubMed: 33262836