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RecombinantRabMAb

Recombinant Anti-CD160 antibody [EPR23644-24] (ab274374)

Submit a review Submit a question References (1)
Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)
  • Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)
  • Anti-CD160 antibody [EPR23644-24] (ab274374)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23644-24] to CD160
  • Suitable for: Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CD160 antibody [EPR23644-24]
    See all CD160 primary antibodies

  • Description

    Rabbit monoclonal [EPR23644-24] to CD160

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P,IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: Human peripheral blood mononuclear cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab274374 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
Notes
Flow Cyt
1/500.
Application notes
Is unsuitable for ICC/IF,IHC-P,IP or WB.

Target

  • Function

    Receptor showing broad specificity for both classical and non-classical MHC class I molecules.

  • Tissue specificity

    Expressed in spleen, peripheral blood, and small intestine. Expression is restricted to functional NK and T cytotoxic lymphocytes.

  • Sequence similarities

    Contains 1 Ig-like V-type (immunoglobulin-like) domain.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • BY55 antibody
      • BY55_HUMAN antibody
      • CD160 antibody
      • CD160 antigen [Precursor] antibody
      • CD160 antigen antibody
      • CD160 delta Ig antibody
      • CD160 molecule antibody
      • CD160 transmembrane isoform antibody
      • FLJ46513 antibody
      • Natural killer cell receptor BY55 antibody
      • Natural killer cell receptor, immunoglobulin superfamily member antibody
      • NK1 antibody
      • NK28 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)
      Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD160 with ab274374 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730)  isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG or ab274374. Then stained with anti-CD8 alpha conjugated to Pacific blue.

      Gated on viable cells.

    • Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)
      Flow Cytometry - Anti-CD160 antibody [EPR23644-24] (ab274374)

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD160 with ab274374 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Negative control: Monocytes (PMID: 30770415). Monocytes are the SSC high population. No positive signal was detected in this population.

    • Anti-CD160 antibody [EPR23644-24] (ab274374)
      Anti-CD160 antibody [EPR23644-24] (ab274374)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (1)

    Publishing research using ab274374? Please let us know so that we can cite the reference in this datasheet.

    ab274374 has been referenced in 1 publication.

    • Xue Z  et al. VEGFA-Enriched Exosomes from Tendon-Derived Stem Cells Facilitate Tenocyte Differentiation, Migration, and Transition to a Fibroblastic Phenotype. Biomed Res Int 2022:8537959 (2022). PubMed: 36119932

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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