Anti-CD168 antibody [2F2C9] (ab234065)
Key features and details
- Mouse monoclonal [2F2C9] to CD168
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD168 antibody [2F2C9]
See all CD168 primary antibodies -
Description
Mouse monoclonal [2F2C9] to CD168 -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human CD168 aa 306-497. (Expressed in E.coli).
Database link: O75330 -
Positive control
- WB: Human CD168 (aa 306-497) recombinant protein; CD168 (aa 306-497)-hIgGFc transfected HEK-293 cell lysate. Flow Cyt: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
2F2C9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab234065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/500. Predicted molecular weight: 84 kDa.
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Flow Cyt |
1/200 - 1/400.
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Notes |
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WB
1/100 - 1/500. Predicted molecular weight: 84 kDa. |
Flow Cyt
1/200 - 1/400. |
Target
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Function
Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including the focal adhesion kinase occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular-regulated kinase (ERK) activity. -
Tissue specificity
Expressed in breast cancer cell lines and in normal breast tissue. -
Cellular localization
Cell surface. Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 3161 Human
- Omim: 600936 Human
- SwissProt: O75330 Human
- Unigene: 728200 Human
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Alternative names
- CD168 antibody
- CD168 antigen antibody
- HMMR antibody
see all
Images
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All lanes : Anti-CD168 antibody [2F2C9] (ab234065) at 1/100 dilution
Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate
Lane 2 : CD168 (aa 306-497)-hIgGFc transfected HEK-293 cell lysate
Predicted band size: 84 kDa -
Anti-CD168 antibody [2F2C9] (ab234065) at 1/100 dilution + Human CD168 (aa 306-497) recombinant protein
Predicted band size: 84 kDaExpected MW is 48.3 kDa.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling CD168 with ab234065 at 1/200 dilution (green) compared with a negative control (red).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab234065 has not yet been referenced specifically in any publications.