Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)](https://www.abcam.com/ps/products/11/ab11076/Images/ab11076-18737-anti-cd1d-antibody-nor32-nor32-1317-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)")
Key features and details
- Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d
- Suitable for: IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)]
See all CD1d primary antibodies -
Description
Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep
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Immunogen
Fusion protein corresponding to Human CD1d.
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Positive control
- IHC-P: Human skin tissue. Flow Cyt: MOLT4 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
NOR3.2 (NOR3.2/13.17) -
Myeloma
NS0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab11076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
| Notes |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells. -
Tissue specificity
Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues. -
Sequence similarities
Contains 1 Ig-like (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes. - Information by UniProt
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Database links
- Entrez Gene: 912 Human
- Omim: 188410 Human
- SwissProt: P15813 Human
- Unigene: 1799 Human
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Alternative names
- Antigen-presenting glycoprotein CD1d antibody
- CD1.1 antibody
- CD1A antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
IHC image of CD1d staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab11076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
![Flow Cytometry - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)](https://www.abcam.com/ps/products/11/ab11076/Images/ab11076-168619-anti-cd1d-antibody-nor32-nor32-1317-flow-cytometry.jpg)
Flow Cytometry - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)Overlay histogram showing MOLT4 cells stained with ab11076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11076, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)](https://www.abcam.com/ps/products/11/ab11076/Images/ab11076-18738-anti-cd1d-antibody-nor32-nor32-1317-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)Image courtesy of an anonymous Abreview.ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.
Protocols
Datasheets and documents
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Datasheet download
References (10)
ab11076 has been referenced in 10 publications.
- Lehmann N et al. Tumor Lipids of Pediatric Papillary Renal Cell Carcinoma Stimulate Unconventional T Cells. Front Immunol 11:1819 (2020). PubMed: 32973759
- Liu D et al. Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells. Clin Immunol 149:55-64 (2013). PubMed: 23891738
- Ascierto ML et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. J Transl Med 11:145 (2013). IHC-P . PubMed: 23758773
- Xu C et al. Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol 85:539-41 (2010). IHC . PubMed: 20575029
- Allen CE et al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr ; Human . PubMed: 20220088
- Motohashi S et al. A phase I-II study of alpha-galactosylceramide-pulsed IL-2/GM-CSF-cultured peripheral blood mononuclear cells in patients with advanced and recurrent non-small cell lung cancer. J Immunol 182:2492-501 (2009). Flow Cyt ; Human . PubMed: 19201905
- Matsumoto J et al. Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation. Biochem Biophys Res Commun 371:236-41 (2008). IHC-Fr ; Human . PubMed: 18433720
- Kawana K et al. Expression of CD1d and ligand-induced cytokine production are tissue specific in mucosal epithelia of the human lower reproductive tract. Infect Immun 76:3011-8 (2008). PubMed: 18458073
- Kawana K et al. CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. J Biol Chem 282:7368-75 (2007). PubMed: 17215251
- Shimizu K et al. Evaluation of the function of human invariant NKT cells from cancer patients using alpha-galactosylceramide-loaded murine dendritic cells. J Immunol 177:3484-92 (2006). Flow Cyt ; Human . PubMed: 16920991
