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  1. Link

    products/primary-antibodies/cd1d-antibody-nor32-nor321317-ab11076.pdf

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Immunology Adaptive Immunity T Cells CD
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Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

  • Datasheet
Reviews (2)Q&A (1)References (10)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
  • Flow Cytometry - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

Key features and details

  • Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d
  • Suitable for: IHC-P, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG1

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Overview

  • Product name

    Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)]
    See all CD1d primary antibodies
  • Description

    Mouse monoclonal [NOR3.2 (NOR3.2/13.17)] to CD1d
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Sheep
  • Immunogen

    Fusion protein corresponding to Human CD1d.

  • Positive control

    • IHC-P: Human skin tissue. Flow Cyt: MOLT4 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 6.97% L-Arginine
  • Concentration information loading...
  • Purity

    Protein G purified
  • Purification notes

    Purified from tissue culture supernatant.
  • Clonality

    Monoclonal
  • Clone number

    NOR3.2 (NOR3.2/13.17)
  • Myeloma

    NS0
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Immunology
    • Immunoglobulins
    • Receptors
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • T Lymphocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Lymphoid
    • NK Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Dendritic Cell Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Monocytic Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Neutrophil Lineage
    • Stem Cells
    • Hematopoietic Progenitors
    • Myeloid
    • Mast Cell Lineage

Associated products

  • Alternative Versions

    • Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] - BSA and Azide free (ab238657)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human CD1d protein (ab114936)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab11076 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Notes
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt
Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Antigen-presenting protein that binds self and non-self glycolipids and presents them to T-cell receptors on natural killer T-cells.
  • Tissue specificity

    Expressed on cortical thymocytes, on certain T-cell leukemias, and in various other tissues.
  • Sequence similarities

    Contains 1 Ig-like (immunoglobulin-like) domain.
  • Cellular localization

    Cell membrane. Endosome membrane. Lysosome membrane. Subject to intracellular trafficking between the cell membrane, endosomes and lysosomes.
  • Target information above from: UniProt accession P15813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 912 Human
    • Omim: 188410 Human
    • SwissProt: P15813 Human
    • Unigene: 1799 Human
    • Alternative names

      • Antigen-presenting glycoprotein CD1d antibody
      • CD1.1 antibody
      • CD1A antibody
      • CD1d antibody
      • CD1D antigen antibody
      • CD1D antigen d polypeptide antibody
      • CD1d molecule antibody
      • CD1D_HUMAN antibody
      • Cd1d1 antibody
      • differentiation antigen CD1 alpha 3 antibody
      • HMC class I antigen like glycoprotein CD1D antibody
      • Ly 38 antibody
      • MGC34622 antibody
      • R3 antibody
      • R3G1 antibody
      • T cell surface glycoprotein CD1d antibody
      • Thymocyte antigen CD1D antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

      IHC image of CD1d staining in human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA based pH 9.0 solution (epitope retrieval solution 2) for 20 mins. The section was then incubated with ab11076, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    • Flow Cytometry - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
      Flow Cytometry - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)

      Overlay histogram showing MOLT4 cells stained with ab11076 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab11076, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabeled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD1d antibody [NOR3.2 (NOR3.2/13.17)] (ab11076)Image courtesy of an anonymous Abreview.
      ab11076 staining CD1d in human skin tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
      Tissue was fixed with formaldehyde and an antigen retrieval step was performed using EDTA pH 8.0 for 20 minutes at 100°C. Samples were then incubated with ab11076 at a 1/200 dilution for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse/rabbit IgG.

      See Abreview

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    References (10)

    Publishing research using ab11076? Please let us know so that we can cite the reference in this datasheet.

    ab11076 has been referenced in 10 publications.

    • Lehmann N  et al. Tumor Lipids of Pediatric Papillary Renal Cell Carcinoma Stimulate Unconventional T Cells. Front Immunol 11:1819 (2020). PubMed: 32973759
    • Liu D  et al. Medulloblastoma expresses CD1d and can be targeted for immunotherapy with NKT cells. Clin Immunol 149:55-64 (2013). PubMed: 23891738
    • Ascierto ML  et al. Molecular signatures mostly associated with NK cells are predictive of relapse free survival in breast cancer patients. J Transl Med 11:145 (2013). IHC-P . PubMed: 23758773
    • Xu C  et al. Expression of CD1d and presence of invariant NKT cells in classical Hodgkin lymphoma. Am J Hematol 85:539-41 (2010). IHC . PubMed: 20575029
    • Allen CE  et al. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells. J Immunol 184:4557-67 (2010). IHC-Fr ; Human . PubMed: 20220088
    • Motohashi S  et al. A phase I-II study of alpha-galactosylceramide-pulsed IL-2/GM-CSF-cultured peripheral blood mononuclear cells in patients with advanced and recurrent non-small cell lung cancer. J Immunol 182:2492-501 (2009). Flow Cyt ; Human . PubMed: 19201905
    • Matsumoto J  et al. Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation. Biochem Biophys Res Commun 371:236-41 (2008). IHC-Fr ; Human . PubMed: 18433720
    • Kawana K  et al. Expression of CD1d and ligand-induced cytokine production are tissue specific in mucosal epithelia of the human lower reproductive tract. Infect Immun 76:3011-8 (2008). PubMed: 18458073
    • Kawana K  et al. CD1d degradation in Chlamydia trachomatis-infected epithelial cells is the result of both cellular and chlamydial proteasomal activity. J Biol Chem 282:7368-75 (2007). PubMed: 17215251
    • Shimizu K  et al. Evaluation of the function of human invariant NKT cells from cancer patients using alpha-galactosylceramide-loaded murine dendritic cells. J Immunol 177:3484-92 (2006). Flow Cyt ; Human . PubMed: 16920991

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD1d [NOR3.2 (NOR3.2/13.17)] antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: EDTA pH 8, 20 min, 100C
    Permeabilization
    No
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Dec 12 2011

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD1d [NOR3.2 (NOR3.2/13.17)] antibody

    Good
    Abreviews
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Sheep Tissue sections (kidney)
    Specification
    kidney
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate acid
    Permeabilization
    Yes - triton x-100
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: rt°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MRS. Mari Palviainen

    Verified customer

    Submitted Mar 04 2011

    Question

    Thank you for always kind support. We bought your product #AB11076 for IHC (frozen). If you have information about dilution rate, please let me know. Best regards,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 02 2005

    Answer

    Thank you for your enquiry regarding ab11076. For use in IHC (frozen) we recommend using this antibody at a 1:100 dilution or an approximate working concentration of 10ug/ml. However, we always suggest that the user titrates the antibody for use in their own system using the appropriate positive and negative controls to see what works best for them.

    Read More

    Abcam Scientific Support

    Answered on Aug 02 2005

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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