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  1. Link

    products/primary-antibodies/cd21cd35-antibody-4b2-ab243317.pdf

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Immunology Adaptive Immunity B Cells CD
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Recombinant

Recombinant Anti-CD21+CD35 antibody [4B2] (ab243317)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
  • Western blot - Anti-CD21 antibody [4B2] (ab243317)
  • Anti-CD21+CD35 antibody [4B2] (ab243317)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [4B2] to CD21+CD35
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Overview

  • Product name

    Anti-CD21+CD35 antibody [4B2]
    See all CD21+CD35 primary antibodies
  • Description

    Mouse monoclonal [4B2] to CD21+CD35
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is considered to be commercially sensitive.

  • Positive control

    • WB: 2.4G2 whole cell lysate. ICC/IF: WEHI-231 and 2.4G2 cells.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    4B2
  • Isotype

    IgG1
  • Research areas

    • Immunology
    • Adaptive Immunity
    • B Cells
    • CD
    • Immunology
    • Adaptive Immunity
    • T Cells
    • CD
    • Cardiovascular
    • Blood
    • Blood Cell Antigens
    • RBC Antigens
    • Blood Group Antigens
    • Immunology
    • Innate Immunity
    • Myeloid Cells
    • Microbiology
    • Protein
    • Human Protein
    • Receptor

Associated products

  • Alternative Versions

    • Anti-CD21+CD35 antibody [4B2] - BSA and Azide free (ab252778)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
  • Related Products

    • Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab243317 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use a concentration of 0.487 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa).
ICC/IF
Use a concentration of 2.435 µg/ml.
Notes
WB
Use a concentration of 0.487 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa).
ICC/IF
Use a concentration of 2.435 µg/ml.
Application notes
Is unsuitable for Flow Cyt,IHC-Fr,IHC-P or IP.

Target

  • Relevance

    CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. The CD35 gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined.
  • Cellular localization

    Membrane; Single pass type I membrane protein.
  • Database links

    • Entrez Gene: 12902 Mouse
    • Entrez Gene: 289395 Rat
    • SwissProt: P19070 Mouse
    • Alternative names

      • C3 binding protein antibody
      • C3b/C4b receptor antibody
      • C3BR antibody
      • C3DR antibody
      • C4BR antibody
      • CD 21 antibody
      • CD 35 antibody
      • CD21 antigen antibody
      • CD35 antigen antibody
      • Complement C3d receptor antibody
      • Complement component (3b/4b) receptor 1 antibody
      • Complement component (3d/Epstein Barr virus) receptor 2 antibody
      • Complement component receptor 1 antibody
      • Complement component receptor 2 antibody
      • Complement receptor 1 antibody
      • Complement receptor type 1 antibody
      • Complement receptor type 2 antibody
      • Cr 2 antibody
      • CR1 antibody
      • Cr2 antibody
      • EBV receptor antibody
      • Epstein Barr virus receptor antibody
      • KN antibody
      • Knops blood group antigen antibody
      • SLEB9 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
      Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
      Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).

      Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.

      Negative control: EL4 (PMID: 10453011).

    • Western blot - Anti-CD21 antibody [4B2] (ab243317)
      Western blot - Anti-CD21 antibody [4B2] (ab243317)
      All lanes : Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/ml

      Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
      Lane 2 : 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution

      Predicted band size: 113 kDa
      Observed band size: 155 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

      Exposure time: 3 minutes.

      Negative control: EL4 (PMID: 10453011).

    • Anti-CD21+CD35 antibody [4B2] (ab243317)
      Anti-CD21+CD35 antibody [4B2] (ab243317)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab243317? Please let us know so that we can cite the reference in this datasheet.

    ab243317 has been referenced in 1 publication.

    • Kulik L  et al. A new mouse anti-mouse complement receptor type 2 and 1 (CR2/CR1) monoclonal antibody as a tool to study receptor involvement in chronic models of immune responses and disease. Mol Immunol 63:479-88 (2015). PubMed: 25457881

    Customer reviews and Q&As

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