Recombinant Anti-CD21+CD35 antibody [4B2] (ab243317)
![Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)](https://www.abcam.com/ps/products/243/ab243317/Images/ab243317-365718-anti-cd21-antibody-4b2-immunocytochemistry-rat.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [4B2] to CD21+CD35
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-CD21+CD35 antibody [4B2]
See all CD21+CD35 primary antibodies -
Description
Mouse monoclonal [4B2] to CD21+CD35 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-Fr,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is considered to be commercially sensitive.
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Positive control
- WB: 2.4G2 whole cell lysate. ICC/IF: WEHI-231 and 2.4G2 cells.
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General notes
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
4B2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab243317 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.487 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa).
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| ICC/IF |
Use a concentration of 2.435 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.487 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 113 kDa). |
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ICC/IF
Use a concentration of 2.435 µg/ml. |
Target
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Relevance
CD21 also known as complement receptor 2 (CR2), C3d receptor or EBV receptor is a 140 kDa protein. CD21 is a glycosylated type I transmembrane protein consisting of an extracellular face of a series of 15 or 16 CCP domains. CD21 is the receptor for complement components C3d and iC3b as well as the Epstein-Barr virus (EBV) glycoprotein gp350/220. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signalling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders. The CD35 gene encodes a membrane glycoprotein found on peripheral blood cells, glomerular podocytes, and follicular dendritic cells. The protein is a receptor for complement components C3b and C4b and regulates the activity of the complement cascade. Variation in this protein is the basis of the Knops blood group system. The two most common alleles, F and S, differ by 8 exons and are thought to be the result of an unequal crossover event. A secreted form of the protein present in plasma has been described, but its full length nature has not been determined. -
Cellular localization
Membrane; Single pass type I membrane protein. -
Database links
- Entrez Gene: 12902 Mouse
- Entrez Gene: 289395 Rat
- SwissProt: P19070 Mouse
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Alternative names
- C3 binding protein antibody
- C3b/C4b receptor antibody
- C3BR antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 2.4G2 (Rat B cell lymphoma B lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in 2.4G2 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)](https://www.abcam.com/ps/products/243/ab243317/Images/ab243317-365717-1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD21 antibody [4B2] (ab243317)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized WEHI-231 (Mouse B cell lymphoma B lymphocyte) or EL4 (Mouse lymphoma T lymphocyte) cells labeling CD21 with ab243317 at 2.435µg/ml, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing membranous and weak cytoplasmic staining in WEHI-231 cell line. Tubulin is detected with ab179504 Anti-beta IV Tubulin antibody - Microtubule Marker at 1/200 dilution, followed by ab150080 AlexaFluor®594 Goat anti-Rabbit secondary (red). The nuclear counter stain is DAPI (blue).
Secondary antibody only control: Used PBS instead of primary antibody, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution.
Negative control: EL4 (PMID: 10453011).
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![Western blot - Anti-CD21 antibody [4B2] (ab243317)](https://www.abcam.com/ps/products/243/ab243317/Images/ab243317-365716-anti-cd21-antibody-4b2-western-blot-mouse.jpg)
Western blot - Anti-CD21 antibody [4B2] (ab243317)All lanes : Anti-CD21+CD35 antibody [4B2] (ab243317) at 0.487 µg/ml
Lane 1 : EL4 (mouse lymphoma T lymphocyte), whole cell lysate
Lane 2 : 2.4G2 (rat B cell lymphoma B lymphocyte), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG at 1/10000 dilution
Predicted band size: 113 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted?Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
Negative control: EL4 (PMID: 10453011).
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![Anti-CD21+CD35 antibody [4B2] (ab243317)](https://www.abcam.com/ps/products/243/ab243317/Images/ab243317-4-benefits-of-recombinant-antibodies.png)
Anti-CD21+CD35 antibody [4B2] (ab243317)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab243317 has been referenced in 1 publication.
- Kulik L et al. A new mouse anti-mouse complement receptor type 2 and 1 (CR2/CR1) monoclonal antibody as a tool to study receptor involvement in chronic models of immune responses and disease. Mol Immunol 63:479-88 (2015). PubMed: 25457881