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Validated using a knockout cell lineRecombinant

Recombinant Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Flow Cytometry - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
  • Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP265] to CD276 - BSA and Azide free
  • Suitable for: ICC/IF, Flow Cyt, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

APC HRP PE Unconjugated

Overview

  • Product name

    Anti-CD276 antibody [SP265] - BSA and Azide free
    See all CD276 primary antibodies

  • Description

    Rabbit monoclonal [SP265] to CD276 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, Flow Cyt, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Hs 700T cell lysate. IHC-P: Human ovarian adenocarcinoma, lung squamous cell carcinoma, renal cell carcinoma, liver hepatoma, bladder transitional cell carcinoma, cervical squamous cell carcinoma, skin squamous cell carcinoma, skin melanoma, Hodgkin's lymphoma and prostate adenocarcinoma tissue. Flow cytometry: PC-3 cells. ICC/IF: HEK293 and LNCaP cells

  • General notes

    ab272011 is the carrier-free version of ab227679.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab272011 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/100.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

For antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
Notes
ICC/IF
1/100.
Flow Cyt
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration.

For antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes.
WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.

Target

  • Function

    May participate in the regulation of T-cell-mediated immune response. May play a protective role in tumor cells by inhibiting natural-killer mediated cell lysis as well as a role of marker for detection of neuroblastoma cells. May be involved in the development of acute and chronic transplant rejection and in the regulation of lymphocytic activity at mucosal surfaces. Could also play a key role in providing the placenta and fetus with a suitable immunological environment throughout pregnancy. Both isoform 1 and isoform 2 appear to be redundant in their ability to modulate CD4 T-cell responses. Isoform 2 is shown to enhance the induction of cytotoxic T-cells and selectively stimulates interferon gamma production in the presence of T-cell receptor signaling.

  • Tissue specificity

    Ubiquitous but not detectable in peripheral blood lymphocytes or granulocytes. Weakly expressed in resting monocytes. Expressed in dendritic cells derived from monocytes. Expressed in epithelial cells of sinonasal tissue. Expressed in extravillous trophoblast cells and Hofbauer cells of the first trimester placenta and term placenta.

  • Sequence similarities

    Belongs to the immunoglobulin superfamily. BTN/MOG family.
    Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 2 Ig-like V-type (immunoglobulin-like) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 4Ig B7 H3 antibody
    • 4Ig-B7-H3 antibody
    • AU016588 antibody
    • B7 H3 antibody
    • B7 homolog 3 antibody
    • B7-H3 antibody
    • B7H3 antibody
    • B7RP-2 antibody
    • CD_antigen=CD276 antibody
    • CD276 antibody
    • CD276 antigen antibody
    • CD276 molecule antibody
    • CD276_HUMAN antibody
    • Costimulatory molecule antibody
    • Flags: Precursor antibody
    • PSEC0249 antibody
    • UNQ309/PRO352 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human renal cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    This data was developed using the same antibody clone in a different buffer formulation (ab227679).

    ab227679 staining CD276 in wild-type HEK293 cells (top panel) and CD276 knockout HEK293 cells (ab266658) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab227679 at 1/100 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human ovarian adenocarcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunocytochemistry/ Immunofluorescence - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Immunocytochemistry/ Immunofluorescence analysis of LNCaP (Human prostate carcinoma epithelial cell) cells labeling CD276  with purified ab227679 at 1/25 (9.24 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Flow Cytometry - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Flow Cytometry - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Flow cytometric analysis of PC-3 (human prostate adenocarcinoma cell line) cell line labeling CC276 with ab227679 at 1/400 (green) compared with a rabbit isotype control (blue).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human Hodgkin's lymphoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human skin melanoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human skin squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human cervical squamous cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

    Formalin-fixed, paraffin-embedded human liver hepatoma tissue stained for CD276 with ab227679 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227679).
  • Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)
    Anti-CD276 antibody [SP265] - BSA and Azide free (ab272011)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab272011? Please let us know so that we can cite the reference in this datasheet.

ab272011 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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For licensing inquiries, please contact partnerships@abcam.com