Anti-CD3 antibody [PS1] (ab699)
Key features and details
- Mouse monoclonal [PS1] to CD3
- Suitable for: IHC-Fr, IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-CD3 antibody [PS1]
See all CD3 primary antibodies -
Description
Mouse monoclonal [PS1] to CD3 -
Host species
Mouse -
Specificity
This antibody, when used in conjunction with UCHL-1, is regarded as a reliable pan T-cell marker for immunophenotyping of lymphomas (See reference). Exceptions include some of the more aggressive, high-grade T cell lymphomas, which may not express detectable antigen. This antibody does not react with B-cells. -
Tested applications
Suitable for: IHC-Fr, IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Fusion protein corresponding to CD3. Prokaryotic fusion protein corresponding to the epsilon chain of CD3
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Positive control
- Tonsil or T-cell lymphoma. IHC-P: Human colon tissue and colorectal mucosa tissue. IHC-Fr: Human skin tissue.
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General notes
This antibody works well when used in combination with ab972 - Antigen retrieval solution - heat mediated high pH 9.5.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: Proprietary preservative
Constituents: PBS, Carrier protein
Proprietary preservative that is not sodium azide or thimerosal, protein carrier. -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
PS1 -
Myeloma
unknown -
Isotype
IgG2a -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | (1) |
Use at an assay dependent concentration.
|
| IHC-P | (3) |
Use at an assay dependent concentration.
|
| ICC/IF | (3) |
Use at an assay dependent concentration.
|
| WB | (4) |
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. PubMed: 21654633
|
| Notes |
|---|
|
IHC-Fr
Use at an assay dependent concentration. |
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IHC-P
Use at an assay dependent concentration. |
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ICC/IF
Use at an assay dependent concentration. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 19 kDa. PubMed: 21654633 |
Target
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Function
The CD3 complex mediates signal transduction. -
Involvement in disease
Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. -
Sequence similarities
Contains 1 ITAM domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 915 Human
- Entrez Gene: 916 Human
- Entrez Gene: 917 Human
- Entrez Gene: 919 Human
- Omim: 186740 Human
- Omim: 186780 Human
- Omim: 186790 Human
- Omim: 186830 Human
see all -
Alternative names
- 4930549J05Rik antibody
- A430104F18Rik antibody
- AW552088 antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab699 has been referenced in 25 publications.
- Zhan X et al. Glioma stem-like cells evade interferon suppression through MBD3/NuRD complex-mediated STAT1 downregulation. J Exp Med 217:N/A (2020). PubMed: 32181805
- Liu Z et al. IL-21 promotes osteoblastic differentiation of human valvular interstitial cells through the JAK3/STAT3 pathway. Int J Med Sci 17:3065-3072 (2020). PubMed: 33173427
- Wang B et al. Loss of survival advantage for deficient mismatch repair in patients with advanced colorectal cancer may be caused by changes in prognostic value of CD8+T cell. World J Surg Oncol 18:196 (2020). PubMed: 32767974
- Wang Y et al. Expression and clinical significance of PD-L1, B7-H3, B7-H4 and VISTA in craniopharyngioma. J Immunother Cancer 8:N/A (2020). PubMed: 32958683
- Liang H et al. The proatherosclerotic function of indoleamine 2, 3-dioxygenase 1 in the developmental stage of atherosclerosis. Signal Transduct Target Ther 4:23 (2019). PubMed: 31637003