Recombinant Anti-CD31 antibody [EPR17260-263] (ab222783)
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.com/ps/products/222/ab222783/Images/ab222783-477257-anti-cd31-antibody-epr17260-263-immunocytochemistry.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17260-263] to CD31
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
-
Product name
Anti-CD31 antibody [EPR17260-263]
See all CD31 primary antibodies -
Description
Rabbit monoclonal [EPR17260-263] to CD31 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
-
Positive control
- WB: bEND.3 whole cell lysate; Mouse platelet and lung lysates; Rat lung lysate. ICC/IF: bEND.3 cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17260-263 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab222783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
1/100.
It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
|
| WB | (2) |
1/2000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 82 kDa).
In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates. Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
| Notes |
|---|
|
ICC/IF
1/100. It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
|
WB
1/2000. Detects a band of approximately 110-130 kDa (predicted molecular weight: 82 kDa). In WB, under our testing conditions, we observe an additional band (~50 kDa) for some human, mouse and rat cell and tissue lysates. Treat samples with PNGase F or phosphatase to confirm the specificity of bands if necessary. The observed band size of CD31 may not the same as predicted MWs in WB due to the different forms and modifications of CD31. Ms Isoform 1-4: 69.8-81.3 kDa (predicted) Observed band size is around 130 kDa Positive Control: HUVEC and Jurkat cell lysates (ab7899); Human spleen and kidney tissue lysate. Negative Control: NIH/3T3 whole cell lysate (ab7179). |
Target
-
Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
-
Database links
- Entrez Gene: 18613 Mouse
- Entrez Gene: 29583 Rat
- SwissProt: Q08481 Mouse
- SwissProt: Q3SWT0 Rat
- Unigene: 343951 Mouse
- Unigene: 1878 Rat
-
Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [EPR17260-263] (ab222783)
Immunofluorescent analysis of 4% paraformaldehyde-fixed. 0.1% Triton X-100 permeabilized bEND.3 (mouse brain endothelioma cell line) and NIH/3T3 (mouse embyro fibroblast cell line) cells labeling CD31 with ab222783 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining on bEND.3 cells. Negative control: NIH/3T3 (PMID: 1429859).
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
-
![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.com/ps/products/222/ab222783/Images/ab222783-281562-anti-cd31-antibody-epr17260-263-western-blot.jpg)
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)All lanes : Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution
Lane 1 : bEND.3 (mouse brain endothelioma cell line) whole cell lysate
Lane 2 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Negative control: NIH/3T3 (PMID: 1429859).
-
![Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.com/ps/products/222/ab222783/Images/ab222783-281563-anti-cd31-antibody-epr17260-263-western-blot.jpg)
Western blot - Anti-CD31 antibody [EPR17260-263] (ab222783)All lanes : Anti-CD31 antibody [EPR17260-263] (ab222783) at 1/2000 dilution
Lane 1 : Mouse platelet lysate
Lane 2 : Rat lung lysate
Lane 3 : Mouse lung lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 110-130 kDa why is the actual band size different from the predicted?Exposure time : Lane 1-2: 3 minutes; Lane 3: 5 seconds.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 10448867).
-
![Anti-CD31 antibody [EPR17260-263] (ab222783)](https://www.abcam.com/ps/products/222/ab222783/Images/ab222783-4-benefits-of-recombinant-antibodies.png)
Anti-CD31 antibody [EPR17260-263] (ab222783)
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
Certificate of Compliance
References (39)
ab222783 has been referenced in 39 publications.
- Li Z et al. A fully biodegradable polydioxanone occluder for ventricle septal defect closure. Bioact Mater 24:252-262 (2023). PubMed: 36632501
- Lin J et al. Single cell analysis reveals inhibition of angiogenesis attenuates the progression of heterotopic ossification in Mkx-/- mice. Bone Res 10:4 (2022). PubMed: 34996891
- Chen S et al. Human Umbilical Cord Blood-Derived CD133+CD34+ Cells Protect Retinal Endothelial Cells and Ganglion Cells in X-Irradiated Rats through Angioprotective and Neurotrophic Factors. Front Cell Dev Biol 10:801302 (2022). PubMed: 35223834
- Zhang W et al. Astragaloside IV Alleviates Infarction Induced Cardiomyocyte Injury by Improving Mitochondrial Morphology and Function. Front Cardiovasc Med 9:810541 (2022). PubMed: 35265681
- Cai C et al. Empagliflozin attenuates cardiac microvascular ischemia/reperfusion through activating the AMPKα1/ULK1/FUNDC1/mitophagy pathway. Redox Biol 52:102288 (2022). PubMed: 35325804