Anti-CD31 antibody [TLD-3A12] (ab64543)
Key features and details
- Mouse monoclonal [TLD-3A12] to CD31
- Suitable for: IHC-Fr
- Reacts with: Rat
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD31 antibody [TLD-3A12]
See all CD31 primary antibodies -
Description
Mouse monoclonal [TLD-3A12] to CD31 -
Host species
Mouse -
Specificity
ab64543 recognises rat PECAM-1 (CD31). It also partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. -
Tested applications
Suitable for: IHC-Frmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Rat
Predicted to work with: Pig, Rhesus monkeyDoes not react with: Human
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Immunogen
Tissue, cells or virus corresponding to Rat CD31. Activated, Lewis rat derived microglial cells
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Positive control
- Rat spleen; activated microglial cells.
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General notes
This antibody has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Primary antibody notes
This antibody has been shown to partially block the proliferative response of antigen-specific CD4+ T cells to antigen-presenting cells and relevant antigen. -
Clonality
Monoclonal -
Clone number
TLD-3A12 -
Myeloma
Sp2 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64543 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr | (2) |
1/10 - 1/100.
A fixation with acetone or methanol is recommended. Do not fix with PFA. |
Notes |
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IHC-Fr
1/10 - 1/100. A fixation with acetone or methanol is recommended. Do not fix with PFA. |
Target
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Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 29583 Rat
- SwissProt: Q3SWT0 Rat
- Unigene: 1878 Rat
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Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
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Immunohistochemical staining of rat brain cryosection with Mouse anti Rat CD31 (ab64543)
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Immunohistochemistry (Frozen sections) - Anti-CD31 antibody [TLD-3A12] (ab64543)This image is courtesy of Valley Lasry.ab64543 staining CD31 in rat brain tissue by Immunohistochemistry (Frozen sections). The sections were fixed in paraformaldehyde prior to blocking with 5% donkey serum, BSA 1%, Triton 1.5% for 2 hours at room temperature. The primary antibody was diluted 1/200 in PBS/5% Donkey serum and incubated with the sample for 12 hours at 4°C. An Alexa Fluor® 555-conjugated donkey anti-mouse polyclonal was used as the secondary antibody, diluted 1/200.
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Immunohistochemical staining of rat spleen cryosection with Mouse anti Rat CD31 (ab64543)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (70)
ab64543 has been referenced in 70 publications.
- Kim BH et al. Restoration of Homeostasis in the Tracheal Mucosa After Thyroid Surgery in a Rat Model. In Vivo 36:161-169 (2022). PubMed: 34972711
- Adamo A et al. Blood Vessel Detection Algorithm for Tissue Engineering and Quantitative Histology. Ann Biomed Eng 50:387-400 (2022). PubMed: 35171393
- Zhi D et al. Mechanically reinforced biotubes for arterial replacement and arteriovenous grafting inspired by architectural engineering. Sci Adv 8:eabl3888 (2022). PubMed: 35294246
- Huang LY et al. Healthy Serum-Derived Exosomes Improve Neurological Outcomes and Protect Blood-Brain Barrier by Inhibiting Endothelial Cell Apoptosis and Reversing Autophagy-Mediated Tight Junction Protein Reduction in Rat Stroke Model. Front Cell Neurosci 16:841544 (2022). PubMed: 35308117
- Wang H et al. Anti-Sca-1 antibody-functionalized vascular grafts improve vascular regeneration via selective capture of endogenous vascular stem/progenitor cells. Bioact Mater 16:433-450 (2022). PubMed: 35415291