Recombinant Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
![Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)](https://www.abcam.com/ps/products/252/ab252265/Images/ab252265-1-anti-cd31-antibody-wm59-immunocytochemistry-huvec.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [WM59] to CD31 - BSA and Azide free
- Suitable for: ICC/IF, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD31 antibody [WM59] - BSA and Azide free
See all CD31 primary antibodies -
Description
Mouse monoclonal [WM59] to CD31 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- ICC: HUVEC cells. Flow Cyt: HUVEC cells.
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General notes
ab252265 is the carrier-free version of ab218.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
WM59 -
Isotype
IgG1 -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab252265 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
|
| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. It is recommended to incubate cells with 0.1% Triton-X for 5 min to detect nuclear antigen. Use 0.3M glycine to quench autofluorescence caused by aldehydes. Positive Control: HUVEC cells |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC). -
Tissue specificity
Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Isoform Long predominates in all tissues examined. Isoform Delta12 is detected only in trachea. Isoform Delta14-15 is only detected in lung. Isoform Delta14 is detected in all tissues examined with the strongest expression in heart. Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U937 histiocytic lymphoma cell lines (at protein level). -
Sequence similarities
Contains 6 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
The Ig-like C2-type domains 2 and 3 contribute to formation of the complex with BDKRB2 and in regulation of its activity. -
Post-translational
modificationsPhosphorylated on Ser and Tyr residues after cellular activation. In endothelial cells Fyn mediates mechanical-force (stretch or pull) induced tyrosine phosphorylation. -
Cellular localization
Membrane. Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells and Cell junction. Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells. - Information by UniProt
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Database links
- Entrez Gene: 5175 Human
- Omim: 173445 Human
- SwissProt: P16284 Human
- Unigene: 376675 Human
- Unigene: 514412 Human
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Alternative names
- Adhesion molecule antibody
- CD31 antibody
- CD31 antigen antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HUVEC cells labelling CD31 with ab218 at 1/100 dilution (10.62µg/ml), followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor® 488) antibody at 1/1000 (2µg/ml) dilution (Green). Confocal image showing cytoplasmic and membranous staining in HUVEC cell line. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) at a 1/500 dilution (Red). The nuclear counterstain was DAPI (Blue).
Negative control cell: HEK-293 (PMID: 27097314).
Negative control 1: ab218 at a 1/100 dilution (10.62µg/ml) followed by ab150080 at a 1/500 dilution (4µg/ml).
Negative control 2: ab179513 at a 1/500 dilution (4µg/ml) followed by ab150113 at a 1/1000 dilution (4µg/ml).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218).
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![Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)](https://www.abcam.com/ps/products/252/ab252265/Images/ab252265-2-anti-cd31-antibody-wm59-flow-cytometry-hek-293.jpg)
Flow Cytometry - Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)Flow cytometric analysis of HEK 293 (human embryonic kidney epithelial cell) (Left panel) / HUVEC (human umbilical vein endothelial cell) (Right panel) cells labelling CD31 with ab218 at 1/1000 dilution (1.062µg/ml) (Red) compared with a mouse monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti mouse IgG (Alexa Fluor® 488, ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK 293 (PMID: 27097314).
Gated on viable cells.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218).
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![Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)](https://www.abcam.com/ps/products/252/ab252265/Images/ab252265-3-benefits-of-recombinant-antibodies.png)
Anti-CD31 antibody [WM59] - BSA and Azide free (ab252265)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (6)
ab252265 has been referenced in 6 publications.
- Lundqvist A et al. The Arachidonate 15-Lipoxygenase Enzyme Product 15-HETE Is Present in Heart Tissue from Patients with Ischemic Heart Disease and Enhances Clot Formation. PLoS One 11:e0161629 (2016). PubMed: 27552229
- Bushway ME et al. Morphological and phenotypic analyses of the human placenta using whole mount immunofluorescence. Biol Reprod 90:110 (2014). PubMed: 24648400
- Wicki A et al. Targeting tumor-associated endothelial cells: anti-VEGFR2 immunoliposomes mediate tumor vessel disruption and inhibit tumor growth. Clin Cancer Res 18:454-64 (2012). PubMed: 22065082
- Oeztuerk-Winder F et al. Regulation of human lung alveolar multipotent cells by a novel p38a MAPK/miR-17-92 axis. EMBO J 31:3431-41 (2012). PubMed: 22828869
- Nagano M et al. Identification of functional endothelial progenitor cells suitable for the treatment of ischemic tissue using human umbilical cord blood. Blood 110:151-60 (2007). PubMed: 17379743
- MacFadyen JR et al. Endosialin (TEM1, CD248) is a marker of stromal fibroblasts and is not selectively expressed on tumour endothelium. FEBS Lett 579:2569-75 (2005). PubMed: 15862292
