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Anti-CD34 antibody (ab64480)

Reviews (2)Q&A (2)References (9)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (ab64480)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (ab64480)

Key features and details

  • Rabbit polyclonal to CD34
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-CD34 antibody [EPR2999] (ab110643)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-CD34 antibody
    See all CD34 primary antibodies

  • Description

    Rabbit polyclonal to CD34

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat

  • Immunogen

    Synthetic peptide within Human CD34 aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P28906

  • Positive control

    • Human lung and tonsil tissue.

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64480 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P (2)
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use 10 mM sodium citrate pH 6.0, boiling for 10 minutes followed by 20 minutes cooling.
Notes
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Use 10 mM sodium citrate pH 6.0, boiling for 10 minutes followed by 20 minutes cooling.

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.

  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.

  • Sequence similarities

    Belongs to the CD34 family.

  • Developmental stage

    On early hematopoietic progenitor cells.

  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (ab64480)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (ab64480)
      ab64480, at a 1/100 dilution, staining CD34 in formalin fixed, paraffin embedded human lung tissue by Immunohistochemistry.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (ab64480)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody (ab64480)This image is courtesy of an anonymous Abreview
      ab64480 staining CD34 in Human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 1 hour at 20°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/50) for 12 hours at 4°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

      See Abreview

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (9)

    Publishing research using ab64480? Please let us know so that we can cite the reference in this datasheet.

    ab64480 has been referenced in 9 publications.

    • Qiu L  et al. Pro-Angiogenic and Pro-Inflammatory Regulation by lncRNA MCM3AP-AS1-Mediated Upregulation of DPP4 in Clear Cell Renal Cell Carcinoma. Front Oncol 10:705 (2020). PubMed: 32714856
    • Wang Y  et al. cRGD-functionalized nanoparticles for combination therapy of anti-endothelium dependent vessels and anti-vasculogenic mimicry to inhibit the proliferation of ovarian cancer. Acta Biomater 94:495-504 (2019). PubMed: 31252171
    • Rodríguez-Hernández MA  et al. Molecular characterization of autophagic and apoptotic signaling induced by sorafenib in liver cancer cells. J Cell Physiol 234:692-708 (2018). PubMed: 30132846
    • Choi G  et al. CD99 Is Strongly Expressed in Basal Cells of the Normal Adult Epidermis and Some Subpopulations of Appendages: Comparison with Developing Fetal Skin. J Pathol Transl Med 50:361-8 (2016). PubMed: 27498544
    • Tokunaga T  et al. FGF-2 Stimulates the Growth of Tenogenic Progenitor Cells to Facilitate the Generation of Tenomodulin-Positive Tenocytes in a Rat Rotator Cuff Healing Model. Am J Sports Med 43:2411-22 (2015). PubMed: 26311443
    • Lupinacci RM  et al. Intrahepatic lymphatic invasion but not vascular invasion is a major prognostic factor after resection of colorectal cancer liver metastases. World J Surg 38:2089-96 (2014). PubMed: 24663482
    • Xu H  et al. Effects of acupuncture at GV20 and ST36 on the expression of matrix metalloproteinase 2, aquaporin 4, and aquaporin 9 in rats subjected to cerebral ischemia/reperfusion injury. PLoS One 9:e97488 (2014). ICC/IF ; Rat . PubMed: 24828425
    • Kucera T  et al. Pericyte coverage of fetoplacental vessels in pregnancies complicated by Type 1 diabetes mellitus. Placenta 31:1120-2 (2010). IHC-P ; Human . PubMed: 20947164
    • Gartland A  et al. Septoclast deficiency accompanies postnatal growth plate chondrodysplasia in the toothless (tl) osteopetrotic, colony-stimulating factor-1 (CSF-1)-deficient rat and is partially responsive to CSF-1 injections. Am J Pathol 175:2668-75 (2009). IHC-P ; Mouse . PubMed: 19893052

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody

     Inconclusive
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM sodium citrate Ph 6
    Sample
    Rat Tissue sections (Lung, Kidneys)
    Specification
    Lung, Kidneys
    Permeabilization
    No
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Oct 15 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CD34 antibody

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Sep 13 2010

    Question

    Thanks again for helping with secondary antibody selection yesterday for an extracellular epitope for rats. I have not yet received the article you referred me to, but was thinking it over again how we can possibly prevent a non-specific binding of secondary anti –body using your 8158. Do you have any information supporting that your other CD43, your cat. # 64480 would work in ICC-IF? I would not be able to use it for cell selection, but at least can may be able to quantify hematopoietic progenitors in peripheral blood MNC.

    Read More
    Answer

    That might be a better choice than ab8158, insofar as you avoid the issue of reactivity of the anti-rat IgG secondary with your sample. For instance, the anti-rabbit IgG secondary ab96889, conjugated to Dylight 488 (similar to Alexa 488) will recognize ab64480 but not IgG rat IgG in your sample. However, it has not been tested for ICC/IF (cytochemistry), only IHC (histochemistry) on paraffin-embedded tissue, so we do not guarantee it for that application. They are similar, but not identical. Further, the immunogen is from a cytoplasmic domain, so the cells will need to be fixed and permeabilized to allow the antibody access to the cytoplasm. Have you considered Thy-1 as a marker? This paper suggest it may be useful: Haematologica. 1998 Jul;83(7):587-92. http://www.ncbi.nlm.nih.gov/pubmed/9718862?dopt=AbstractPlus We have several antibodies to this target, inculding clone MRC OX-7 which we have unconjgate (ab225) and conjugated to FITC (ab226).

    Read More

    Question

    Dear technical team,   Our customer is looking for CD34 antibody to test rat sample with IHC-P.   I have searched on the abcam's website but there was no product for rat sample.   If you have antibody which has possibility to react rat sample, please let me know.   Thank you.   Best regards,

    Read More
    Answer

    Thank you for contacting us. Although we do not currently have any antibodies specifically directed against Rat CD34, we have an antibody that would be predicted to react with this protein. Ab64480 is a rabbit polyclonal antibody against human CD34. The region of the immunogen however shares 100% homology with that of Rat CD34 (SwissProt number B1PLB1). This antibody has also been characterise with IHC-P and therefore may be suitable for your customer. If they were to purchase this antibody for testing in rat samples they would be eligible for a testing discount, more details below: www.abcam.com/collaborationdiscount If your customer would be interested in this offer, please get in contact before purchase as the code needs to be issued prior to purchase. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com