For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    products/primary-antibodies/cd34-antibody-ep373y-bsa-and-azide-free-ab198395.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Type Marker Neuron marker Soma marker
Share by email
RecombinantRabMAb

Recombinant Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

  • Datasheet
  • Certificate of Compliance
Reviews (4) Submit a question References (25)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunoprecipitation - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Western blot - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • OI-RD Scanning - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
  • Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP373Y] to CD34 - BSA and Azide free
  • Suitable for: IHC-Fr, Flow Cyt (Intra), IHC-P, WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 HRP PE Unconjugated

You may also be interested in

Conjugation
Product image
APC Conjugation Kit - Lightning-Link® (ab201807)
Primary
Product image
Anti-c-Kit antibody [YR145] - BSA and Azide free (ab216450)
Primary
Product image
Anti-CD34 antibody [EP373Y] (ab81289)

View more associated products

Overview

  • Product name

    Anti-CD34 antibody [EP373Y] - BSA and Azide free
    See all CD34 primary antibodies
  • Description

    Rabbit monoclonal [EP373Y] to CD34 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, Flow Cyt (Intra), IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Sheep, Dog, African bush elephant
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow Cyt (intra): TF-1 cells. WB: TF-1 cell lysate. IHC-P: Human kidney tissue; Mouse normal brain, prostate and kidney tissues; Rat kidney tissue. ICC/IF: HUVEC cells; Human embryonic stem cell-derived endothelial cells. IHC-Fr: Mouse and rat lung tissue. Rat kidney. IP: TF-1 cell lysate.
  • General notes

    ab198395 is the carrier-free version of ab81289.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Dissociation constant (KD)

    KD = 1.15 x 10 -10 M
    Learn more about KD
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP373Y
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Soma marker
    • Immunology
    • Cell Type Markers
    • CD
    • Myeloid Cells
    • Stem Cells
    • Lineage Markers
    • Mesoderm
    • Stem Cells
    • Mesenchymal Stem Cells
    • Negative Markers
    • Stem Cells
    • Hematopoietic Progenitors
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Heart
    • Cardiogenesis
    • Stem cells
    • Stem Cells
    • Hematopoietic Progenitors
    • Hematopoietic Stem Cells
    • HSC markers
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Developmental Biology
    • Lineage specification
    • Mesoderm
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-CD34 antibody [EP373Y] (ab195013)
    • HRP Anti-CD34 antibody [EP373Y] (ab195017)
    • PE Anti-CD34 antibody [EP373Y] (ab223930)
    • Anti-CD34 antibody [EP373Y] (ab81289)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CD34 protein (ab126924)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab198395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr (1)
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P (2)
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 40 kDa).

ab198395 is not suitable for rat and mouse species in WB application.

ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
Notes
IHC-Fr
Use at an assay dependent concentration.
Flow Cyt (Intra)
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB
Use at an assay dependent concentration. Detects a band of approximately 120 kDa (predicted molecular weight: 40 kDa).

ab198395 is not suitable for rat and mouse species in WB application.

ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

Target

  • Function

    Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins.
  • Tissue specificity

    Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
  • Sequence similarities

    Belongs to the CD34 family.
  • Developmental stage

    On early hematopoietic progenitor cells.
  • Post-translational
    modifications

    Highly glycosylated.
    Phosphorylated on serine residues by PKC.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession P28906 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 947 Human
    • Entrez Gene: 12490 Mouse
    • Entrez Gene: 305081 Rat
    • Omim: 142230 Human
    • SwissProt: P28906 Human
    • SwissProt: Q64314 Mouse
    • Unigene: 374990 Human
    • Unigene: 29798 Mouse
    • Alternative names

      • CD34 antibody
      • CD34 antigen antibody
      • CD34 molecule antibody
      • CD34_HUMAN antibody
      • Cluster designation 34 antibody
      • cluster of differentiation 34 antibody
      • Hematopoietic progenitor cell antigen CD34 antibody
      • HPCA1 antibody
      • Mucosialin antibody
      • OTTHUMP00000034733 antibody
      • OTTHUMP00000034734 antibody
      see all

    Images

    • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Intracellular Flow Cytometry analysis of TF-1 (human erythroleukemia) cells labeling CD34 with purified ab81289 at 1/50 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control. 

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289). 

       

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)This image is courtesy of an Abreview submitted by Rudolf Jung.
      Paraformaldehyde-fixed, paraffin-embedded mouse kidney tissue stained for CD34 using ab81289 at 1/200 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

      See Abreview

    • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      This data was developed using ab81289, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat kidney tissue labeling CD34 with ab81289 at 1/50 (11.04 µg/mL) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat kidney. is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.<\p>

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Frozen sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      This data was developed using ab81289, the same antibody clone in a different buffer formulation.

      Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat lung tissue labeling CD34 with ab81289 at 1/50 (11.04 µg/mL) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on rat lung. is observed. The nuclear counterstain was DAPI (Blue).

      Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 dilution.<\p>

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)This image is courtesy of an anonymous Abreview.
      Paraformaldehyde-fixed, paraffin-embedded mouse prostate tissue stained for CD34 using ab81289 at 1/150 dilution in immunohistochemical analysis.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

      See Abreview

    • Immunoprecipitation - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunoprecipitation - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      CD34 was immunoprecipitated from TF-1 (Human bone marrow erythroleukemia cells) cells using purified ab81289 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab81289. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as secondary antibody at 1/1000 dilution. Blocking and dilution buffer and concentration: 5% NFDM/TBST

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Western blot - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Western blot - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      This data was developed using ab81289, the same antibody clone in a different buffer formulation. Different batches of ab81289 were tested on TF-1 (Human Erythroleukemia erythroblast) lysate at 0.02 µg/ml. 15 µg of lysate was loaded in each lane. Bands observed at 120 kDa.
    • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Clone EP373Y (ab198395) has been successfully conjugated by Abcam. This image was generated using Anti-CD34 antibody [EP373Y] (Alexa Fluor® 488). Please refer to ab195013 for protocol details. 

      Overlay histogram showing HUVEC cells stained with ab195013 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab195013, 1/500 dilution) for 30 min at 22°C. 

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Alexa Fluor® 488 (ab199091) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter. 

      This antibody gave a positive signal in HUVEC cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions. 

       

       

    • Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Flow Cytometry (Intracellular) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Clone EP373Y (ab198395) has been successfully conjugated by Abcam. This image was generated using Anti-CD34 antibody [EP373Y] (PE). Please refer to ab223930 for protocol details. 

      Overlay histogram showing HUVEC cells stained with ab223930 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab223930, 1/500 dilution) for 30 min at 22°C. 

      Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. 

      Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter. 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Immunocytochemistry/Immunofluorescence analysis of HUVEC (Human umbilical vein endothelial cell line) cells labelling CD34 with purified ab81289 at 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (ab150077) (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counter stain.

      Control: Secondary antibody only, ab150120, an Alexa Fluor® 488-conjugated goat anti-mouse IgG (1/500).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CD34 with purified ab81289 at 1/2500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) (ab97051) was used as the secondary antibody at 1/500. Negative control using PBS instead of primary antibody. Counter stained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human angiosarcoma labeling CD34 with unpurified ab81289 at 1/100-1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunocytochemistry/ Immunofluorescence - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)Image from F?ldes G et al. PLoS One. 2010 May 5;5(5):e10501 Fig 3.

      Immunocytochemistry/Immunofluorescence analysis of human embryonic stem cell-derived endothelial cells labeling CD34 with unpurified ab81289 at 1/200. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.2% aTriton X-100. An Alexa Fluor® 647-conjugated secondary antibody was used at a 1/400 dilution. DAPI (blue) was used as the nuclear counter stain.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal kidney vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal uterus vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      IHC image of CD34 staining in Mouse normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with unpurified ab81289, 1/250 dilution, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • OI-RD Scanning - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      OI-RD Scanning - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81289).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

      This IHC data was generated using the same anti-CD34 antibody clone, EP373Y, in a different buffer formulation (cat# ab81289).

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Normal tonsil vessels tissue labeling CD34 with unpurified ab81289 at 1/100-1/250.

       

    • Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)
      Anti-CD34 antibody [EP373Y] - BSA and Azide free (ab198395)

    Protocols

    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (25)

    Publishing research using ab198395? Please let us know so that we can cite the reference in this datasheet.

    ab198395 has been referenced in 25 publications.

    • Schwabenland M  et al. Deep spatial profiling of human COVID-19 brains reveals neuroinflammation with distinct microanatomical microglia-T-cell interactions. Immunity 54:1594-1610.e11 (2021). PubMed: 34174183
    • Mei X  et al. Association between glioblastoma cell-derived vessels and poor prognosis of the patients. Cancer Commun (Lond) 40:211-221 (2020). PubMed: 32359215
    • Storer MA  et al. Acquisition of a Unique Mesenchymal Precursor-like Blastema State Underlies Successful Adult Mammalian Digit Tip Regeneration. Dev Cell 52:509-524.e9 (2020). PubMed: 31902657
    • Sun C  et al. A reactive oxygen species scoring system predicts cisplatin sensitivity and prognosis in ovarian cancer patients. BMC Cancer 19:1061 (2019). PubMed: 31703584
    • Carr MJ  et al. Mesenchymal Precursor Cells in Adult Nerves Contribute to Mammalian Tissue Repair and Regeneration. Cell Stem Cell 24:240-256.e9 (2019). PubMed: 30503141
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Immunohistochemistry (Frozen sections) abreview for Anti-CD34 antibody [EP373Y] - BSA and Azide free

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Sciatic nerve)
    Permeabilization
    Yes - 1%BSA and 0.25% Triton in PBS
    Specification
    Sciatic nerve
    Blocking step
    3%BSA and 0.05% Triton in PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    Submitted Nov 30 2020

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2023 Abcam plc. All rights reserved.