Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)](https://www.abcam.com/ps/products/213/ab213058/Images/ab213058-264520-ab213058IHCa.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)")
Key features and details
- Mouse monoclonal [HPCA1/1171] to CD34 - BSA and Azide free
- Suitable for: Flow Cyt, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free
See all CD34 primary antibodies -
Description
Mouse monoclonal [HPCA1/1171] to CD34 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD34.
Database link: P28906 -
Positive control
- Human angiosarcoma and tonsil tissues; KG-1 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
HPCA1/1171 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab213058 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 0.5-1µg for 106 cells.
(in 0.1 ml). |
|
| IHC-P |
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 min at RT). |
| Notes |
|---|
|
Flow Cyt
Use 0.5-1µg for 106 cells. (in 0.1 ml). |
|
IHC-P
Use a concentration of 2 - 4 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 min at RT). |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human angiosarcoma tissue labeling CD34 with ab213058 at 4 µg/ml.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)](https://www.abcam.com/ps/products/213/ab213058/Images/ab213058-264519-ab213058IHCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling CD34 with ab213058 at 4 µg/ml.
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![Flow Cytometry - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)](https://www.abcam.com/ps/products/213/ab213058/Images/ab213058-264518-ab213058FC.jpg)
Flow Cytometry - Anti-CD34 antibody [HPCA1/1171] - BSA and Azide free (ab213058)Flow cytometric analysis of KG-1 cells labeling CD34 with ab213058 at 1 µg/106 cells (red), compared to an isotype control (green).
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab213058 has been referenced in 3 publications.
- Zhang Y et al. Experimental study of ASCs combined with POC-PLA patch for the reconstruction of full-thickness chest wall defects. PLoS One 12:e0182971 (2017). Flow Cyt ; Rabbit . PubMed: 28800620
- Cao XF et al. Therapeutic effects of hepatocyte growth factor-overexpressing dental pulp stem cells on liver cirrhosis in a rat model. Sci Rep 7:15812 (2017). PubMed: 29150644
- Rawal S et al. Integration of mesenchymal stem cells into islet cell spheroids improves long-term viability, but not islet function. Islets 9:87-98 (2017). PubMed: 28662368
