Anti-CD34 antibody [QBEND-10] (ab8536)
![Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-14252-anti-cd34-antibody-qbend-10-flow-cytometry.jpg "Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)")
Key features and details
- Mouse monoclonal [QBEND-10] to CD34
- Suitable for: Flow Cyt, IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
Overview
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Product name
Anti-CD34 antibody [QBEND-10]
See all CD34 primary antibodies -
Description
Mouse monoclonal [QBEND-10] to CD34 -
Host species
Mouse -
Specificity
This antibody recognizes the CD34 antigen, some cross reactions with vascular associated adventitia and some basement membranes. Ultrastructure studies, using an indirect immuno-gold technique on ultra thin sections of human umbilical artery, have revealed that this antigen is associated to a degree with the basoluminal surfaces of endothelium and shows a slight reaction with an intracytoplasmic vesicular compartment. Predominant staining is on the endothelial cell membrane. QBEND-10 immunoprecipitates a glycoprotein with a relative MW 110kD expressed on haematopoietic cells and on the established myeloid leukaemic cell line KG1A. -
Tested applications
Suitable for: Flow Cyt, IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Does not react with: Rat, Sheep, Cow, Dog -
Immunogen
Tissue, cells or virus corresponding to Human CD34. Human endothelial vesicles
Database link: P28906 -
Epitope
This antibody's target epitope is distinct from those of other anti-CD34 reactive mabs as demonstrated by its lack of sensitivity to neuraminidase treatment, and fluorescence staining profiles during flow cytometry analysis of target cell populations. -
Positive control
- tonsil, bone marrow
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 1% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
QBEND-10 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab8536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/50.
Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
| IHC-P | (4) |
1/50 - 1/100. Do not perform antigen retrieval.
|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa.
|
| Notes |
|---|
|
Flow Cyt
1/50. Mmethanol fixed cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
IHC-P
1/50 - 1/100. Do not perform antigen retrieval. |
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 40 kDa. |
Target
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Function
Possible adhesion molecule with a role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. Presents carbohydrate ligands to selectins. -
Tissue specificity
Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues. -
Sequence similarities
Belongs to the CD34 family. -
Developmental stage
On early hematopoietic progenitor cells. -
Post-translational
modificationsHighly glycosylated.
Phosphorylated on serine residues by PKC. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 947 Human
- Omim: 142230 Human
- SwissProt: P28906 Human
- Unigene: 374990 Human
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Alternative names
- CD34 antibody
- CD34 antigen antibody
- CD34 molecule antibody
see all
Images
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Flow Cytometry - Anti-CD34 antibody [QBEND-10] (ab8536)Overlay histogram showing Jurkat cells stained with ab8536 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab8536, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a significantly decreased signal in Jurkat cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback. -
![Western blot - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-14253-anti-cd34-antibody-qbend-10-western-blot.jpg)
Western blot - Anti-CD34 antibody [QBEND-10] (ab8536)Anti-CD34 antibody [QBEND-10] (ab8536) at 5 µg/ml + Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 100 kDa (possible glycosylated form) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-219201-ab8536ihcp20x.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-268998-ab8536.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview by Carl Hobbs.ab8536 staining CD34 in human kidney tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/2000 in blocking buffer) for 16 hours at 21°C. A Biotin-conjugated goat anti-mouse IgG polyclonal (1/300) was used as the secondary antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-219202-ab8536ihcp40x.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labelling CD34 with ab8536 at 1µg/ml (1:1000). Blood vessels show positive staining.
Detection system polymeric HRP. HIER with Pressure cooker pH6.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-273517-ab8536IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon tissue sections labeling CD34 with ab8536 at 1ug/ml.
Detection system polymeric HRP. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536-273518-ab853616832.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an abreview submitted by Manoj Kumar Valluru.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human umbilical cord labeling CD34 with ab8536 at 1/1000 dilution. Tissue was fixed in formaldehyde and permeabilized with PBS. Heat mediated antigen retrieval was performed using a citrate pH 6 buffer. Tissue was blocked with 5% serum for 1 hour at room temperature. A biotinylated polyclonal goat anti-mouse secondary antibody was used at 1/100 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)](https://www.abcam.com/ps/products/8/ab8536/Images/ab8536_1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD34 antibody [QBEND-10] (ab8536)This image is courtesy of an Abreview submitted by Dr Jose Javier Martin De Llanoab8536 at 1/500 staining human umbilical cord tissue sections by IHC-P. The tissue was paraformaldehyde fixed and blocked with horse serum before incubation with the antibody for 16 hours. A biotinylated goat anti-mouse IgG was used as the secondary.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (56)
ab8536 has been referenced in 56 publications.
- Han X et al. Basal microvilli define the metabolic capacity and lethal phenotype of pancreatic cancer. J Pathol 253:304-314 (2021). PubMed: 33159698
- Heylmann D et al. Comparison of DNA repair and radiosensitivity of different blood cell populations. Sci Rep 11:2478 (2021). PubMed: 33510180
- Koo HS et al. Non-invasive Intrauterine Administration of Botulinum Toxin A Enhances Endometrial Angiogenesis and Improves the Rates of Embryo Implantation. Reprod Sci 28:1671-1687 (2021). PubMed: 33650094
- Gutjahr JC et al. CD44 engagement enhances acute myeloid leukemia cell adhesion to the bone marrow microenvironment by increasing VLA-4 avidity. Haematologica 106:2102-2113 (2021). PubMed: 32616529
- Khalaf NB et al. Vascular endothelial ERp72 is involved in the inflammatory response in a rat model of skeletal muscle injury. Mol Med Rep 23:N/A (2021). PubMed: 33398381