Recombinant Anti-CD40L antibody [AT161-10] (ab281905)
![Immunocytochemistry - Anti-CD40L antibody [AT161-10] (ab281905)](https://www.abcam.com/ps/products/281/ab281905/Images/ab281905-1-AntiCD40L-antibody-immunocytochemistry-peripheral-blood-monocytes-human.jpg "Immunocytochemistry - Anti-CD40L antibody [AT161-10] (ab281905)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [AT161-10] to CD40L
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
Overview
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Product name
Anti-CD40L antibody [AT161-10]
See all CD40L primary antibodies -
Description
Mouse monoclonal [AT161-10] to CD40L -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human CD40L. Raised by immunizing mice with Chinese hamster ovary (CHO) cells expressing human CD40L.
Database link: P29965 -
Positive control
- Flow cyt: Human peripheral blood leukocytes. ICC: Human peripheral blood monocyte cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
AT161-10 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab281905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
1/100.
|
|
| ICC |
Use a concentration of 10 µg/ml.
|
| Notes |
|---|
|
Flow Cyt
1/100. |
|
ICC
Use a concentration of 10 µg/ml. |
Target
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Function
Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL-4. Involved in immunoglobulin class switching.
Release of soluble CD40L from platelets is partially regulated by GP IIb/IIIa, actin polymerization, and an matrix metalloproteinases (MMP) inhibitor-sensitive pathway. -
Tissue specificity
Specifically expressed on activated CD4+ T-lymphocytes. -
Involvement in disease
Defects in CD40LG are the cause of X-linked immunodeficiency with hyper-IgM type 1 (HIGM1) [MIM:308230]; also known as X-linked hyper IgM syndrome (XHIM). HIGM1 is an immunoglobulin isotype switch defect characterized by elevated concentrations of serum IgM and decreased amounts of all other isotypes. Affected males present at an early age (usually within the first year of life) recurrent bacterial and opportunistic infections, including Pneumocystis carinii pneumonia and intractable diarrhea due to cryptosporidium infection. Despite substitution treatment with intravenous immunoglobulin, the overall prognosis is rather poor, with a death rate of about 10% before adolescence. -
Sequence similarities
Belongs to the tumor necrosis factor family. -
Post-translational
modificationsThe soluble form derives from the membrane form by proteolytic processing.
N-linked glycan is a mixture of high mannose and complex type. Glycan structure does not influence binding affinity to CD40.
Not O-glycosylated. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 959 Human
- Omim: 300386 Human
- SwissProt: P29965 Human
- Unigene: 592244 Human
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Alternative names
- CD154 antibody
- CD40 antigen ligand antibody
- CD40 ligand antibody
see all
Images
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Immunocytochemistry - Anti-CD40L antibody [AT161-10] (ab281905)
Immunofluorescence analysis of paraformaldehyde-fixed human peripheral blood monocytes labeling CD40L with the chimeric rabbit Iof this antibody at 10 µg/ml, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom chimeric antibody, DAPI, merged channels and an isotype control. The isotype control was stained with Anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.
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![Flow Cytometry - Anti-CD40L antibody [AT161-10] (ab281905)](https://www.abcam.com/ps/products/281/ab281905/Images/ab281905-2-AntiCD40L-antibody-flowcytometry-peripheral-blood-leukocytes-human.jpg)
Flow Cytometry - Anti-CD40L antibody [AT161-10] (ab281905)Flow cytometric analysis of Human peripheral blood leukocytes labeling CD40L with an Anti-Fluorescein IgG antibody (isotype control, black line) or the rabbit chimera version of this antibody (blue line) at 1/100 dilution for 1 hour at room temperature. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at 1/1000 dilution and cells analysed using a flow-cytometer.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab281905 has not yet been referenced specifically in any publications.