Recombinant Anti-CD41 antibody [EPR17876] (ab181582)
![Western blot - Anti-CD41 antibody [EPR17876] (ab181582)](https://www.abcam.com/ps/products/181/ab181582/Images/ab181582-284369-anti-cd41-antibody-epr17876-western-blot.jpg "Western blot - Anti-CD41 antibody [EPR17876] (ab181582)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17876] to CD41
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat
Related conjugates and formulations
Overview
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Product name
Anti-CD41 antibody [EPR17876]
See all CD41 primary antibodies -
Description
Rabbit monoclonal [EPR17876] to CD41 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat platelet and spleen tissue lysate; mouse platelet, lung and spleen tissue lysate. IHC: Mouse and rat spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17876 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
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| IHC-P |
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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| Notes |
|---|
|
WB
1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa). |
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IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Integrin alpha-IIb/beta-3 is a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. It recognizes the sequence R-G-D in a wide array of ligands. It recognizes the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin alpha-IIb/beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen. This step leads to rapid platelet aggregation which physically plugs ruptured endothelial cell surface. -
Tissue specificity
Isoform 1 and isoform 2 were identified in platelets and megakaryocytes, but not in reticulocytes or in Jurkat and U937 white blood cell line. Isoform 3 is expressed by leukemia, prostate adenocarcinoma and melanoma cells but not by platelets or normal prostate or breast epithelial cells. -
Involvement in disease
Defects in ITGA2B are a cause of Glanzmann thrombasthenia (GT) [MIM:273800]; also known as thrombasthenia of Glanzmann and Naegeli. GT is the most common inherited disease of platelets. It is an autosomal recessive disorder characterized by mucocutaneous bleeding of mild-to-moderate severity and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. GT has been classified clinically into types I and II. In type I, platelets show absence of the glycoprotein IIb/beta-3 complexes at their surface and lack fibrinogen and clot retraction capability. In type II, the platelets express the glycoprotein IIb/beta-3 complex at reduced levels (5-20% controls), have detectable amounts of fibrinogen, and have low or moderate clot retraction capability. The platelets of GT 'variants' have normal or near normal (60-100%) expression of dysfunctional receptors. -
Sequence similarities
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 16399 Mouse
- Entrez Gene: 685269 Rat
- SwissProt: Q9QUM0 Mouse
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Alternative names
- antigen CD41 antibody
- BDPLT16 antibody
- BDPLT2 antibody
see all
Images
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Western blot - Anti-CD41 antibody [EPR17876] (ab181582)All lanes : Anti-CD41 antibody [EPR17876] (ab181582) at 1/1000 dilution
Lane 1 : Rat platelet lysate
Lane 2 : Mouse platelet lysate
Lane 3 : Mouse lung tissue lysate
Lane 4 : Mouse spleen tissue lysate
Lane 5 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
Lanes 1-2 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 3-5 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 113 kDa
Observed band size: 113 kDaBlocking/Dilution: 5% NFDM/TBST
Exposure: Lanes 1, 3, 4: 3 minutes; lane 2: 10 seconds; Lane 5: 1 minute.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR17876] (ab181582)](https://www.abcam.com/ps/products/181/ab181582/Images/ab181582-284377-anti-cd41-antibody-epr17876-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR17876] (ab181582)Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD41 with ab181582 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic staining on megakaryocytes and thrombocytes of mouse spleen (PMID: 28133558). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Antigen Retrieval was heat mediated using 0.01M Tris-EDTA, pH 9.0 or 0.01M Sodium Citrate Buffer, pH 6.0.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR17876] (ab181582)](https://www.abcam.com/ps/products/181/ab181582/Images/ab181582-284378-anti-cd41-antibody-epr17876-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD41 antibody [EPR17876] (ab181582)Immunohistochemical analysis of paraffin-embedded rat spleen tissue labeling CD41 with ab181582 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP), ready to use. Cytoplasmic staining on megakaryocytes and thrombocytes of rat spleen (PMID: 28133558). Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP), ready to use.
Antigen Retrieval was heat mediated using 0.01M Tris-EDTA, pH 9.0 or 0.01M Sodium Citrate Buffer, pH 6.0.
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
-
![Anti-CD41 antibody [EPR17876] (ab181582)](https://www.abcam.com/ps/products/181/ab181582/Images/ab181582-4-benefits-of-recombinant-antibodies.png)
Anti-CD41 antibody [EPR17876] (ab181582)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab181582 has been referenced in 4 publications.
- Maderna C et al. A murine model of cerebral cavernous malformations with acute hemorrhage. iScience 25:103943 (2022). PubMed: 35265815
- Jin P et al. A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance. Theranostics 11:2278-2296 (2021). PubMed: 33500725
- Ma R et al. TMAO: a potential mediator of clopidogrel resistance. Sci Rep 11:6580 (2021). PubMed: 33753834
- Kanack AJ et al. Platelet and myeloid cell phenotypes in a rat model of Fabry disease. FASEB J 35:e21818 (2021). PubMed: 34320241