Recombinant Anti-CD42b antibody [SP202] (ab227669)
![Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-330617-anti-cd42b-antibody-sp202-immunohistochemistry.jpg "Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SP202] to CD42b
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-CD42b antibody [SP202]
See all CD42b primary antibodies -
Description
Rabbit monoclonal [SP202] to CD42b -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human spleen, bone marrow, stomach adenocarcinoma and pancreatic adenocarcinoma tissue. IHC-Fr: Mouse and Rat spleen tissue.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SP202 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227669 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | (2) |
1/100.
Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
| IHC-Fr |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
IHC-P
1/100. Antigen retrieval: Boil tissue section in EDTA buffer, pH 8.0 for 10 minutes followed by cooling at room temperature for 20 minutes. |
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IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium. -
Involvement in disease
Non-arteritic anterior ischemic optic neuropathy
Bernard-Soulier syndrome
Bernard-Soulier syndrome A2, autosomal dominant
Pseudo-von Willebrand disease -
Sequence similarities
Contains 7 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain. -
Post-translational
modificationsGlycocalicin, which is approximately coextensive with the extracellular part of the molecule, is cleaved off by calpain during platelet lysis. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 2811 Human
- Entrez Gene: 14723 Mouse
- Entrez Gene: 691992 Rat
- Omim: 606672 Human
- SwissProt: P07359 Human
- SwissProt: O35930 Mouse
- Unigene: 1472 Human
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Alternative names
- Antigen CD42b alpha antibody
- Antigen CD42b-alpha antibody
- BDPLT1 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)
Immunohistochemistry (Frozen) analysis of rat spleen tissue section labeling CD42b with purified ab227669. Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-290402-anti-cd42b-antibody-sp202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)Formalin-fixed, paraffin-embedded human spleen tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-330616-anti-cd42b-antibody-sp202-immunohistochemistry.jpg)
Immunohistochemistry (Frozen sections) - Anti-CD42b antibody [SP202] (ab227669)Immunohistochemistry (Frozen) analysis of mouse spleen tissue section labeling CD42b with purified ab227669. Sections were fixed in 4% paraformaldehyde and permeabilized with 0.2% Triton X-100. Antigen retrieval was heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody. DAPI was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-290403-anti-cd42b-antibody-sp202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-290404-anti-cd42b-antibody-sp202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)Formalin-fixed, paraffin-embedded human pancreatic adenocarcinoma tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-290405-anti-cd42b-antibody-sp202-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD42b antibody [SP202] (ab227669)Formalin-fixed, paraffin-embedded human bone marrow tissue stained for CD42b with ab227669 at 1/100 dilution in immunohistochemical analysis.
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![Anti-CD42b antibody [SP202] (ab227669)](https://www.abcam.com/ps/products/227/ab227669/Images/ab227669-7-benefits-of-recombinant-antibodies.png)
Anti-CD42b antibody [SP202] (ab227669)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab227669 has not yet been referenced specifically in any publications.