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RecombinantRabMAb

Recombinant Anti-CD43 antibody [SP55] (ab101533)

Submit a review Submit a question References (1)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (ab101533)
  • Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)
  • Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
  • Western blot - Anti-CD43 antibody [SP55] (ab101533)
  • Anti-CD43 antibody [SP55] (ab101533)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP55] to CD43
  • Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alkaline Phosphatase APC Carrier Free HRP PE

Overview

  • Product name

    Anti-CD43 antibody [SP55]
    See all CD43 primary antibodies

  • Description

    Rabbit monoclonal [SP55] to CD43

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human CD43 aa 200-300 (internal sequence). The exact sequence is proprietary.
    Database link: P16150

  • Positive control

    • IHC-P: Human tonsil and spleen tissue WB: Jurkat cells. Flow: Jurkat cells, IM-9 cells ICC/IF: IM-9 cells

  • General notes

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab101533 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
1/50.
IHC-P
1/200. Antigen retrieval is recommended: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB
1/200.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
Notes
ICC/IF
1/50.
IHC-P
1/200. Antigen retrieval is recommended: Boil tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
WB
1/200.
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response.

  • Tissue specificity

    Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas.

  • Post-translational
    modifications

    Glycosylated; has a high content of sialic acid and O-linked carbohydrate structures.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 43 antibody
      • CD43 antibody
      • CD43 antigen antibody
      • Galactoglycoprotein antibody
      • GALGP antibody
      • GPL 115 antibody
      • GPL115 antibody
      • Human gene for sialophorin antibody
      • Leucocyte sialoglycoprotein antibody
      • LEUK_HUMAN antibody
      • Leukocyte large sialoglycoprotein antibody
      • Leukocyte sialoglycoprotein antibody
      • Leukosialin antibody
      • LSN antibody
      • Ly-48 antibody
      • sialophorin (gpL115, leukosialin, CD43) antibody
      • Sialophorin antibody
      • Spn antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human spleen tissue sections labeling CD43 with ab101533 at 1/200 dilution (0.55 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human spleen, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101533 for 30 mins at room temperature.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD43 with ab101533 at 1/200 dilution (0.55 µg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
      The section was incubated with ab101533 for 30 mins at room temperature.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (ab101533)
      Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SP55] (ab101533)
      Immunocytochemistry/ Immunofluorescence analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified ab101533 at 1:50 (2 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)
      Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)
      Flow Cytometry analysis of IM-9 (human multiple myeloma B Lymphoblast) cells labeling CD43 with purified ab101533 at 1:20 dilution (5.05 µg/ml) - Red. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.
    • Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)
      Flow Cytometry - Anti-CD43 antibody [SP55] (ab101533)

      Flow cytometric analysis of rabbit anti-CD43 (SP55) antibody ab101533 (1/100) in Jurkat cells (green) compared to negative control of rabbit IgG (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SP55] (ab101533)
      Staining of Human CD43 in a Formalin/PFA-fixed paraffin-embedded section of Human Tonsil using ab101533 at a dilution of 1/200.
    • Western blot - Anti-CD43 antibody [SP55] (ab101533)
      Western blot - Anti-CD43 antibody [SP55] (ab101533)
      Anti-CD43 antibody [SP55] (ab101533) at 1/25 dilution + Jurkat cell lysate

      Observed band size: 105,160 kDa why is the actual band size different from the predicted?

    • Anti-CD43 antibody [SP55] (ab101533)
      Anti-CD43 antibody [SP55] (ab101533)

    References (1)

    Publishing research using ab101533? Please let us know so that we can cite the reference in this datasheet.

    ab101533 has been referenced in 1 publication.

    • Wei S  et al. Development and validation of a novel fibroblast scoring model for lung adenocarcinoma. Front Oncol 12:905212 (2022). PubMed: 36072807

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com