Recombinant Anti-CD43 antibody [SPN/1766R] (ab218523)
![Immunocytochemistry - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-456468-anti-cd43-antibody-spn-1766r-immunocytochemistry-k562-human.jpg "Immunocytochemistry - Anti-CD43 antibody [SPN/1766R] (ab218523)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SPN/1766R] to CD43
- Suitable for: WB, ICC, Flow Cyt, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-CD43 antibody [SPN/1766R]
See all CD43 primary antibodies -
Description
Rabbit monoclonal [SPN/1766R] to CD43 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC, Flow Cyt, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD43 aa 1-400.
Database link: P16150 -
Positive control
- IHC-P: Human tonsil tissue; WB: K562 cell lysate; ICC and Flow Cyt: K562 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.05% BSA, 99% PBS -
Concentration information loading... -
Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
SPN/1766R -
Isotype
IgG -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab218523 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.
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| ICC |
Use a concentration of 1 - 2 µg/ml.
|
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| Flow Cyt |
Use a concentration of 1 - 2 µg/ml.
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| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. |
|
ICC
Use a concentration of 1 - 2 µg/ml. |
|
Flow Cyt
Use a concentration of 1 - 2 µg/ml. |
|
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
Tissue specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
Post-translational
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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Alternative names
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
Images
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Immunocytochemistry - Anti-CD43 antibody [SPN/1766R] (ab218523)
Immunocytochemistry analysis of K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 µg/mL followed by Goat anti-Rabbit- IgG-CF488 (green). The nuclear counterstain is NucSpot (red).
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![Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-456469-anti-cd43-antibody-spn-1766r-flow-cytometry-k562-human.jpg)
Flow Cytometry - Anti-CD43 antibody [SPN/1766R] (ab218523)Flow Cytometry analysis of PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 with ab218523 at 2 µg/106 cells followed by Goat anti-Rabbit- IgG-CF488 (Blue); Isotype Control (Red).
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![Western blot - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-456470-anti-cd43-antibody-spn-1766r-western-blot-k562-human.jpg)
Western blot - Anti-CD43 antibody [SPN/1766R] (ab218523)Anti-CD43 antibody [SPN/1766R] (ab218523) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Predicted band size: 40 kDaCD43 protein has multiple sites of glycosylation which increases the apparent molecular weight in Western blot.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-274004-anti-cd43-antibody-spn-1766r-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/1766R] (ab218523)Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human tonsil tissue labeling CD43 with ab218523 at 1 μg/ml.
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![Anti-CD43 antibody [SPN/1766R] (ab218523)](https://www.abcam.com/ps/products/218/ab218523/Images/ab218523-2-benefits-of-recombinant-antibodies.png)
Anti-CD43 antibody [SPN/1766R] (ab218523)
Datasheets and documents
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Datasheet download
References (0)
ab218523 has not yet been referenced specifically in any publications.
