Recombinant Anti-CD43 antibody [SPN/2049R] (ab257316)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] (ab257316)](https://www.abcam.com/ps/products/257/ab257316/Images/ab257316-352493-anti-cd43-antibody-spn-2049r-immunohistochemistry-tonsil-human.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] (ab257316)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [SPN/2049R] to CD43
- Suitable for: IHC-P, ICC/IF, WB, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD43 antibody [SPN/2049R]
See all CD43 primary antibodies -
Description
Rabbit monoclonal [SPN/2049R] to CD43 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human CD43.
Database link: P16150 -
Positive control
- WB: K562 cell lysate. IHC-P: Human tonsil tissue. ICC/IF: K562 cells. Flow Cyt: K562 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
SPN/2049R -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab257316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at room temperature. |
|
| ICC/IF |
Use a concentration of 1 - 2 µg/ml.
|
|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa.
|
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| Flow Cyt |
Use 1-2µg for 106 cells.
|
| Notes |
|---|
|
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Incubate with primary antibody for 30 minutes at room temperature. |
|
ICC/IF
Use a concentration of 1 - 2 µg/ml. |
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 40 kDa. |
|
Flow Cyt
Use 1-2µg for 106 cells. |
Target
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Function
One of the major glycoproteins of thymocytes and T lymphocytes. Plays a role in the physicochemical properties of the T-cell surface and in lectin binding. Presents carbohydrate ligands to selectins. Has an extended rodlike structure that could protrude above the glycocalyx of the cell and allow multiple glycan chains to be accessible for binding. Is a counter receptor for SN/Siglec-1 (By similarity). During T-cell activation is actively removed from the T-cell-APC (antigen-presenting cell) contact site thus suggesting a negative regulatory role in adaptive immune response. -
Tissue specificity
Cell surface of thymocytes, T-lymphocytes, neutrophils, plasma cells and myelomas. -
Post-translational
modificationsGlycosylated; has a high content of sialic acid and O-linked carbohydrate structures. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6693 Human
- Omim: 182160 Human
- SwissProt: P16150 Human
- Unigene: 632188 Human
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Alternative names
- CD 43 antibody
- CD43 antibody
- CD43 antigen antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD43 antibody [SPN/2049R] (ab257316)
Formalin-fixed, paraffin-embedded human tonsil tissue labeling CD43 using ab257316 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
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![Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] (ab257316)](https://www.abcam.com/ps/products/257/ab257316/Images/ab257316-352494-anti-cd43-antibody-spn-2049r-immunocytochemistry-k562-human.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CD43 antibody [SPN/2049R] (ab257316)K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257316 at 2 μg/ml, followed by Goat anti-Rabbit IgG CF488 (green) in ICC/IF.
The nuclear counterstain is NucSpot (red).
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![Western blot - Anti-CD43 antibody [SPN/2049R] (ab257316)](https://www.abcam.com/ps/products/257/ab257316/Images/ab257316-352495-anti-cd43-antibody-spn-2049r-western-blot-k562-human.jpg)
Western blot - Anti-CD43 antibody [SPN/2049R] (ab257316)Anti-CD43 antibody [SPN/2049R] (ab257316) at 2 µg/ml + K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Predicted band size: 40 kDa -
![Flow Cytometry - Anti-CD43 antibody [SPN/2049R] (ab257316)](https://www.abcam.com/ps/products/257/ab257316/Images/ab257316-352496-anti-cd43-antibody-spn-2049r-flow-cytometry-k562-human.jpg)
Flow Cytometry - Anti-CD43 antibody [SPN/2049R] (ab257316)PFA-fixed K562 (human chronic myelogenous leukemia cell line from bone marrow) cells labeling CD43 using ab257316, followed by Goat anti-Rabbit IgG CF488 (blue) as compared with an isotype control (red).
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![Anti-CD43 antibody [SPN/2049R] (ab257316)](https://www.abcam.com/ps/products/257/ab257316/Images/ab257316-5-benefits-of-recombinant-antibodies.png)
Anti-CD43 antibody [SPN/2049R] (ab257316)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab257316 has not yet been referenced specifically in any publications.
