Anti-CD47 antibody [CD47/2937] - BSA and Azide free (ab260480)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)](https://www.abcam.com/ps/products/260/ab260480/Images/ab260480-353269-anti-cd47-antibody-cd47-2937-immunohistochemistry-prostate-carcinoma-human-1.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)")
Key features and details
- Mouse monoclonal [CD47/2937] to CD47 - BSA and Azide free
- Suitable for: IHC-P, WB, Protein Array
- Reacts with: Human
- Isotype: IgG2c
Related conjugates and formulations
Overview
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Product name
Anti-CD47 antibody [CD47/2937] - BSA and Azide free
See all CD47 primary antibodies -
Description
Mouse monoclonal [CD47/2937] to CD47 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WB, Protein Arraymore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human CD47 aa 18-135. The exact sequence is proprietary.
Database link: Q08722 -
Positive control
- WB: U-87 and THP-1 cell lysates. IHC-P: Human prostate carcinoma tissue.
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General notes
ab260480 is the carrier-free version of ab260418.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A/G purified -
Purification notes
Purified from bioreactor concentrate. -
Clonality
Monoclonal -
Clone number
CD47/2937 -
Isotype
IgG2c -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab260480 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Primary incubation for 30 minutes at room temperature. |
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| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
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| Protein Array |
Use at an assay dependent concentration.
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| Notes |
|---|
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IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary incubation for 30 minutes at room temperature. |
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WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa. |
|
Protein Array
Use at an assay dependent concentration. |
Target
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Function
Has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. Plays an important role in memory formation and synaptic plasticity in the hippocampus (By similarity). Receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. May play a role in membrane transport and/or integrin dependent signal transduction. May prevent premature elimination of red blood cells. May be involved in membrane permeability changes induced following virus infection. -
Tissue specificity
Very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 961 Human
- Omim: 601028 Human
- SwissProt: Q08722 Human
- Unigene: 446414 Human
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Alternative names
- Antigen identified by monoclonal antibody 1D8 antibody
- Antigenic surface determinant protein OA3 antibody
- CD 47 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)
Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
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![Western blot - Anti-CD47 antibody [CD47/2937] (ab260480)](https://www.abcam.com/ps/products/260/ab260480/Images/ab260480-353271-anti-cd47-antibody-cd47-2937-western-blot-u87-thp1-human.jpg)
Western blot - Anti-CD47 antibody [CD47/2937] (ab260480)All lanes : Anti-CD47 antibody [CD47/2937] (ab260418) at 2 µg/ml
Lane 1 : U-87 (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : THP-1 (human monocytic leukemia cell line) cell lysate
Predicted band size: 35 kDaThis data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
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![Protein Array - Anti-CD47 antibody [CD47/2937] (ab260480)](https://www.abcam.com/ps/products/260/ab260480/Images/ab260480-353272-anti-cd47-antibody-cd47-2937-protein-array--human.jpg)
Protein Array - Anti-CD47 antibody [CD47/2937] (ab260480)Array of >19,000 full-length human proteins, probed with ab260418.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD’s) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD’s) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)](https://www.abcam.com/ps/products/260/ab260480/Images/ab260480-353270-anti-cd47-antibody-cd47-2937-immunohistochemistry-prostate-carcinoma-human.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD47 antibody [CD47/2937] (ab260480)Formalin-fixed, paraffin-embedded human prostate carcinoma tissue labeling CD47 using ab260418 at 2 μg/ml in immunohistochemical analysis.
Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
This data was developed using the same antibody clone in a different formulation containing BSA and azide (ab260418).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab260480 has not yet been referenced specifically in any publications.
