Recombinant Anti-CD5 antibody [EP2952] (ab75877)

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Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP2952] to CD5
Suitable for: WB, IP, IHC-P
Reacts with: Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Carrier Free

Product name

Anti-CD5 antibody [EP2952]
See all CD5 primary antibodies
Description

Rabbit monoclonal [EP2952] to CD5
Host species

Rabbit
Tested applications

Suitable for: WB, IP, IHC-Pmore details
Unsuitable for: Flow Cyt
Species reactivity

Reacts with: Human
Immunogen

Synthetic peptide within Human CD5 aa 50-150. The exact sequence is proprietary.
Positive control
- WB: Human lymph node lysate IHC-P: Human tonsil tissue. IP: Human lymph node lysate
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Dissociation constant (K_D)

K_D = 1.70 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP2952
Isotype

IgG
Research areas

Alternative Versions
- Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
- Alexa Fluor® 568 Anti-CD5 antibody [EP2952] (ab313086)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CD5 protein (denatured) (ab180322)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab75877 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP		1/50.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 55 kDa.
IP 1/50.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Application notes

Is unsuitable for Flow Cyt.

Function

May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.
Sequence similarities

Contains 3 SRCR domains.
Cellular localization

Membrane.
Target information above from: UniProt accession P06127 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 921 Human
- Omim: 153340 Human
- SwissProt: P06127 Human
- Unigene: 58685 Human
Alternative names
- CD 5 antibody
- CD5 antibody
- CD5 antigen (p56 62) antibody
- CD5 antigen antibody
- CD5 molecule antibody
- CD5_HUMAN antibody
- LEU1 antibody
- LyA antibody
- Lymphocyte antigen T1/Leu-1 antibody
- OTTHUMP00000236973 antibody
- T-cell surface glycoprotein CD5 antibody
- T1 antibody
see all

Immunoprecipitation - Anti-CD5 antibody [EP2952] (ab75877)

CD5 was immunoprecipitated from 0.35 mg Human lymph node lysate 10 µg with 75877 at 1/20 dilution (0.6µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1: Human lymph node lysate 10 µg

Lane 2: ab75877 IP in Human lymph node lysate

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75877 in Human lymph node lysate

Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Western blot - Anti-CD5 antibody [EP2952] (ab75877)

Anti-CD5 antibody [EP2952] (ab75877) at 1/1000 dilution (Purified) + Human lymph node lysate at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 55 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

ab75877 showing positive staining in Normal colon lymphocytes tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

ab75877 showing negative staining in Normal brain tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

ab75877 showing negative staining in Normal kidney tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

ab75877 showing negative staining in Skeletal muscle tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (ab75877)

ab75877 showing positive staining in Normal spleen tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
OI-RD Scanning - Anti-CD5 antibody [EP2952] (ab75877)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D
Anti-CD5 antibody [EP2952] (ab75877)

Immunoprecipitation protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab75877? Please let us know so that we can cite the reference in this datasheet.

ab75877 has been referenced in 2 publications.

Xiao LI et al. Multiple myeloma-associated skin light chain amyloidosis: A case of misdiagnosis. Oncol Lett 11:3617-3620 (2016). IHC ; Human . PubMed: 27284363
Lu TX et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . PubMed: 26202875

Customer reviews and Q&As

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Question

I contact you as I would need to have the concentration of one of your antibody. This is apparently a tissue culture supernatant but I would still like to know if you could give me a range of concentration for this antibody. The reference is Ab75877.

Abcam community

Verified customer

Asked on May 31 2012

Answer

According to the datasheet, this antibody is indeed a non-purified cell culture supernatantproduct. That means, along with whole serum and Ascitesproducts, the antibody concentration cannot be determined as there are too many other proteins in this solution as well.

However, we have a table under our Technical FAQs (Question 8, What concentration of primary antibody should I use?) which explains how much specific antibody can be expected in the non-purified products. In this case it is 1 - 3 mg/ml.

Abcam Scientific Support

Answered on May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (2)

Customer reviews and Q&As

Dissociation constant (K_D)