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RecombinantRabMAb

Recombinant Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

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Western blot - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Immunoprecipitation - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • OI-RD Scanning - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
  • Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP2952] to CD5 - BSA and Azide free
  • Suitable for: ICC/IF, IP, IHC-P, WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 568 Unconjugated

Overview

  • Product name

    Anti-CD5 antibody [EP2952] - BSA and Azide free
    See all CD5 primary antibodies

  • Description

    Rabbit monoclonal [EP2952] to CD5 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, IP, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human lymph node lysate IHC-P: Human tonsil tissue. IP: Human lymph node lysate

  • General notes

    ab194821 is the carrier-free version of ab75877.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab194821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
Notes
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Perform heat mediated antigen retrieval using 0.01M Sodium Citrate Buffer, pH 6.0 before commencing with IHC staining protocol.
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    May act as a receptor in regulating T-cell proliferation. CD5 interacts with CD72/LYB-2.

  • Sequence similarities

    Contains 3 SRCR domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CD 5 antibody
      • CD5 antibody
      • CD5 antigen (p56 62) antibody
      • CD5 antigen antibody
      • CD5 molecule antibody
      • CD5_HUMAN antibody
      • LEU1 antibody
      • LyA antibody
      • Lymphocyte antigen T1/Leu-1 antibody
      • OTTHUMP00000236973 antibody
      • T-cell surface glycoprotein CD5 antibody
      • T1 antibody
      see all

    Images

    • Western blot - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Western blot - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Anti-CD5 antibody [EP2952] (ab75877) at 1/1000 dilution (Purified) + Human lymph node lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

      Predicted band size: 55 kDa
      Observed band size: 67 kDa why is the actual band size different from the predicted?



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

       

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

      ab75877 showing negative staining in Normal brain tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

      ab75877 showing positive staining in Normal colon lymphocytes tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

      ab75877 showing negative staining in Normal kidney tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

      ab75877 showing negative staining in Skeletal muscle tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

      ab75877 showing positive staining in Normal spleen tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Immunoprecipitation - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

      This data was developed using ab75877, the same antibody clone in a different buffer formulation.

      CD5 was immunoprecipitated from 0.35 mg Human lymph node lysate 10 µg with ab75877 at 1/20 dilution (0.6µg) . VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

      Lane 1: Human lymph node lysate 10 µg

      Lane 2: abab75877 IP in Human lymph node lysate

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75877 in Human lymph node lysate

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    • OI-RD Scanning - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      OI-RD Scanning - Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab75877).

    • Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)
      Anti-CD5 antibody [EP2952] - BSA and Azide free (ab194821)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab194821? Please let us know so that we can cite the reference in this datasheet.

    ab194821 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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