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Immunology Cell Type Markers CD Non-lineage
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RecombinantRabMAb

Recombinant Anti-CD52 antibody [EPR23855-41] (ab259794)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Flow Cytometry - Anti-CD52 antibody [EPR23855-41] (ab259794)
  • Anti-CD52 antibody [EPR23855-41] (ab259794)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23855-41] to CD52
  • Suitable for: Flow Cyt
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

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Overview

  • Product name

    Anti-CD52 antibody [EPR23855-41]
    See all CD52 primary antibodies
  • Description

    Rabbit monoclonal [EPR23855-41] to CD52
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or WB
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Flow cyt: Human PBMCs.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59.94% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23855-41
  • Isotype

    IgG
  • Research areas

    • Immunology
    • Cell Type Markers
    • CD
    • Non-lineage
    • Neuroscience
    • Processes

Associated products

  • Alternative Versions

    • Anti-CD52 antibody [EPR23855-41] - BSA and Azide free (ab275257)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab259794 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
1/500.
Notes
Flow Cyt
1/500.
Application notes
Is unsuitable for ICC/IF,IHC-P or WB.

Target

  • Function

    May play a role in carrying and orienting carbohydrate, as well as having a more specific role.
  • Cellular localization

    Cell membrane.
  • Target information above from: UniProt accession P31358 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1043 Human
    • Omim: 114280 Human
    • SwissProt: P31358 Human
    • Unigene: 276770 Human
    • Alternative names

      • Cambridge pathology 1 antigen antibody
      • CAMPATH 1 antibody
      • CAMPATH 1 antigen antibody
      • CAMPATH-1 antigen antibody
      • CD 52 antibody
      • CD52 antibody
      • CD52 antigen antibody
      • CD52 molecule antibody
      • CD52_HUMAN antibody
      • CDw52 antibody
      • CDW52 antigen antibody
      • Epididymal secretory protein E5 antibody
      • Epididymis secretory sperm binding protein Li 171mP antibody
      • HE 5 antibody
      • He5 antibody
      • Human epididymis-specific protein 5 antibody
      see all

    Images

    • Flow Cytometry - Anti-CD52 antibody [EPR23855-41] (ab259794)
      Flow Cytometry - Anti-CD52 antibody [EPR23855-41] (ab259794)

      Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling CD52 with ab259794 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) (Left) isotype control. 

      Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

      Cells were stained with rabbit IgG or ab259794. Then stained with anti-CD3 conjugated to Alexa Fluor® 647.

      Gated on viable cells.

    • Anti-CD52 antibody [EPR23855-41] (ab259794)
      Anti-CD52 antibody [EPR23855-41] (ab259794)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab259794? Please let us know so that we can cite the reference in this datasheet.

    ab259794 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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