Anti-CD62E antibody (ab18981)
Key features and details
- Rabbit polyclonal to CD62E
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-CD62E antibody
See all CD62E primary antibodies -
Description
Rabbit polyclonal to CD62E -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, Flow Cytmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
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Positive control
- WB: Jurkat cell lysate. Flow Cyt: Jurkat cells. IHC-P: Human placenta
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3631 E-Selectin Antibody.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.03% Proclin 300
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.5% BSA, 0.15% EDTA -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab18981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (2) |
Use at an assay dependent concentration.
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| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB |
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 67 kDa.
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| Flow Cyt |
Use at an assay dependent concentration.
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| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 67 kDa. |
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Flow Cyt
Use at an assay dependent concentration. |
Target
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Function
Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 6 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6401 Human
- Entrez Gene: 25544 Rat
- Omim: 131210 Human
- SwissProt: P16581 Human
- SwissProt: P98105 Rat
- Unigene: 82848 Human
- Unigene: 10359 Rat
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Alternative names
- CD26E antibody
- CD62 antigen-like family member E antibody
- CD62E antibody
see all
Images
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Western blot - Anti-CD62E antibody (ab18981)Anti-CD62E antibody (ab18981) at 1/1000 dilution + Jurkat cell lysate at 80 µg
Secondary
Peroxidase conjugated goat anti-rabbit IgG at 1/10000 dilution
Predicted band size: 67 kDa
Observed band size: 67 kDaBlocking/Dilution buffer: 5% NFDM (1 hr at RT) / TBST.
Incubated with the primary antibody overnight at 4°C. Incubated with the secondary antibody for 1 hour at room temperature.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62E antibody (ab18981)ab18981 IHC staining of human placenta with E-Selectin Polyclonal antibody: Immunohistochemistry of formalin fixed, paraffin-embedded tissue after heat-induced antigen retrieval. 5 μg/ml of antibody used for staining. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen.
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Flow Cytometry - Anti-CD62E antibody (ab18981)Flow cytometric analysis of Jurkat cells using ab18981 (red) with negative controls (black and green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (38)
ab18981 has been referenced in 38 publications.
- García-Bernal D et al. Enforced mesenchymal stem cell tissue colonization counteracts immunopathology. NPJ Regen Med 7:61 (2022). PubMed: 36261464
- Ying Y et al. Activation of ATF4 triggers trabecular meshwork cell dysfunction and apoptosis in POAG. Aging (Albany NY) 13:8628-8642 (2021). PubMed: 33714955
- Li C et al. Chronic Low-Dose Alcohol Consumption Attenuates Post-Ischemic Inflammation via PPAR? in Mice. Int J Mol Sci 22:N/A (2021). PubMed: 34066125
- Williams H et al. Aneurysm severity is suppressed by deletion of CCN4. J Cell Commun Signal 15:421-432 (2021). PubMed: 34080128
- Liu S et al. Shikonin Alleviates Endothelial Cell Injury Induced by ox-LDL via AMPK/Nrf2/HO-1 Signaling Pathway. Evid Based Complement Alternat Med 2021:5881321 (2021). PubMed: 34912465