Recombinant Anti-CD62P antibody [EPR2146(2)] (ab182135)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)](https://www.abcam.com/ps/products/182/ab182135/Images/ab182135-213978-anti-cd62p-antibody-epr21462-immunohistochemistry.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2146(2)] to CD62P
- Suitable for: IHC-P, WB, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CD62P antibody [EPR2146(2)]
See all CD62P primary antibodies -
Description
Rabbit monoclonal [EPR2146(2)] to CD62P -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IPmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal liver, Human placenta and plasma lysates; Human spleen and gliocytoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR2146(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
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| WB |
1/1000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa).
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| IP |
1/30.
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| Notes |
|---|
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IHC-P
1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
WB
1/1000 - 1/10000. Detects a band of approximately 91 kDa (predicted molecular weight: 91 kDa). |
|
IP
1/30. |
Target
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Function
Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. -
Tissue specificity
Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface. -
Involvement in disease
Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. -
Sequence similarities
Belongs to the selectin/LECAM family.
Contains 1 C-type lectin domain.
Contains 1 EGF-like domain.
Contains 9 Sushi (CCP/SCR) domains. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6403 Human
- Omim: 173610 Human
- SwissProt: P16109 Human
- Unigene: 73800 Human
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Alternative names
- Antigen CD62 antibody
- CD62 antibody
- CD62 antigen-like family member P antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)
Immunohistochemical analysis of paraffin-embedded Human gliocytoma tissue labeling CD62P with ab182135 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)](https://www.abcam.com/ps/products/182/ab182135/Images/ab182135-213977-anti-cd62p-antibody-epr21462-immunohistochemistry.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR2146(2)] (ab182135)Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD62P with ab182135 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunoprecipitation - Anti-CD62P antibody [EPR2146(2)] (ab182135)](https://www.abcam.com/ps/products/182/ab182135/Images/ab182135-213979-anti-cd62p-antibody-epr21462-immunoprecipitation.jpg)
Immunoprecipitation - Anti-CD62P antibody [EPR2146(2)] (ab182135)Western blot analysis of immunoprecipitation pellet from Human plasma immunoprecipitated using ab182135 at 1/30 dilution. Secondary antibody Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
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![Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)](https://www.abcam.com/ps/products/182/ab182135/Images/ab182135-213976-anti-cd62p-antibody-epr21462-western-blot.jpg)
Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)Anti-CD62P antibody [EPR2146(2)] (ab182135) at 1/10000 dilution + Human plasma lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 91 kDa -
![Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)](https://www.abcam.com/ps/products/182/ab182135/Images/ab182135-213974-anti-cd62p-antibody-epr21462-western-blot.jpg)
Western blot - Anti-CD62P antibody [EPR2146(2)] (ab182135)All lanes : Anti-CD62P antibody [EPR2146(2)] (ab182135) at 1/2000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : Human placenta lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), HRP at 1/1000 dilution
Predicted band size: 91 kDa -
![Anti-CD62P antibody [EPR2146(2)] (ab182135)](https://www.abcam.com/ps/products/182/ab182135/Images/ab182135-6-benefits-of-recombinant-antibodies.png)
Anti-CD62P antibody [EPR2146(2)] (ab182135)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab182135 has been referenced in 2 publications.
- Wallis S et al. A peptide from the staphylococcal protein Efb binds P-selectin and inhibits the interaction of platelets with leukocytes. J Thromb Haemost 20:729-741 (2022). PubMed: 34846792
- Williams H et al. Aneurysm severity is suppressed by deletion of CCN4. J Cell Commun Signal 15:421-432 (2021). PubMed: 34080128