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RecombinantRabMAb

Recombinant Anti-CD62P antibody [EPR22850-190] (ab255822)

Submit a review Submit a question References (5)
Western blot - Anti-CD62P antibody [EPR22850-190] (ab255822)
  • Western blot - Anti-CD62P antibody [EPR22850-190] (ab255822)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)
  • Anti-CD62P antibody [EPR22850-190] (ab255822)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR22850-190] to CD62P
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-CD62P antibody [EPR22850-190]
    See all CD62P primary antibodies

  • Description

    Rabbit monoclonal [EPR22850-190] to CD62P

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HUVEC, Human serum, Human plasma, Human lung and Mouse platelet lysates. IHC-P: Human stomach, Human spleen and Mouse spleen tissues.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab255822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Notes
WB
1/1000.
IHC-P
1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
Application notes
Is unsuitable for Flow Cyt,ICC/IF or IP.

Target

  • Function

    Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1.

  • Tissue specificity

    Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

  • Involvement in disease

    Defects in SELP may be a cause of susceptibility to ischemic stroke (ISCHSTR) [MIM:601367]; also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors.

  • Sequence similarities

    Belongs to the selectin/LECAM family.
    Contains 1 C-type lectin domain.
    Contains 1 EGF-like domain.
    Contains 9 Sushi (CCP/SCR) domains.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • Antigen CD62 antibody
      • CD62 antibody
      • CD62 antigen-like family member P antibody
      • CD62P antibody
      • GMP 140 antibody
      • GMP-140 antibody
      • GMP140 antibody
      • GMRP antibody
      • Granule membrane protein 140 antibody
      • Granulocyte membrane protein antibody
      • GRMP antibody
      • LECAM 3 antibody
      • LECAM3 antibody
      • Leukocyte endothelial cell adhesion molecule 3 antibody
      • Leukocyte-endothelial cell adhesion molecule 3 antibody
      • LYAM3_HUMAN antibody
      • P Selectin antibody
      • P-selectin antibody
      • PADGEM antibody
      • Platelet activation dependent granule-external membrane protein antibody
      • Platelet alpha granule membrane protein antibody
      • PSEL antibody
      • Selectin P (granule membrane protein 140kDa, antigen CD62) antibody
      • SELP antibody
      see all

    Images

    • Western blot - Anti-CD62P antibody [EPR22850-190] (ab255822)
      Western blot - Anti-CD62P antibody [EPR22850-190] (ab255822)
      All lanes : Anti-CD62P antibody [EPR22850-190] (ab255822) at 1/1000 dilution

      Lane 1 : Human serum
      Lane 2 : Human plasma
      Lane 3 : Human lung lysate
      Lane 4 : Mouse platelet

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution (Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG)

      Observed band size: 140 kDa why is the actual band size different from the predicted?



      Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

      The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24945938).

      Exposure time:  Lane 1:15 seconds; Lanes 2-4:8 seconds.

    • Western blot - Anti-CD62P antibody [EPR22850-190] (ab255822)
      Western blot - Anti-CD62P antibody [EPR22850-190] (ab255822)
      All lanes : Anti-CD62P antibody [EPR22850-190] (ab255822) at 1/1000 dilution

      Lane 1 : HUVEC (human umbilical vein endothelial cell) whole cell lysate
      Lane 2 : HEK-293 (human embryonic kidney epithelial cell) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

      Observed band size: 140,90 kDa why is the actual band size different from the predicted?



       Blocking and Dilution Buffer and concentration: 5% NFDM/TBST.

      140KDa and 90KDa respectively represent glycosylated form and non-glycosylated form. The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 24945938).  Negative control:  HEK-293 (PMID: 7693674).

      Exposure time: 26 seconds

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)

      Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CD62P with ab255822 at 1/100 dilution (5.89 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in megakaryocytes and platelets of mouse spleen (PMID: 2472431). The section was incubated with ab255822 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND&~153;,RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)

      Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling CD62P with ab255822 at 1/500 dilution (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in platelets of human spleen (PMID: 2472431). The section was incubated with ab255822 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD62P antibody [EPR22850-190] (ab255822)

      Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling CD62P with ab255822 at 1/500 dilution (1.178 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in blood vessels of human stomach (PMID: 2472431). The section was incubated with ab255822 for 15 mins at RT. The immunostaining was performed on a Leica Biosystems BOND®RX instrument. Counterstained with Hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-CD62P antibody [EPR22850-190] (ab255822)
      Anti-CD62P antibody [EPR22850-190] (ab255822)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (5)

    Publishing research using ab255822? Please let us know so that we can cite the reference in this datasheet.

    ab255822 has been referenced in 5 publications.

    • Musiu C  et al. Fatal cytokine release syndrome by an aberrant FLIP/STAT3 axis. Cell Death Differ 29:420-438 (2022). PubMed: 34518653
    • Chen X  et al. The phosphatase PTEN links platelets with immune regulatory functions of mouse T follicular helper cells. Nat Commun 13:2762 (2022). PubMed: 35589797
    • Wang C  et al. Targeted delivery of fat extract by platelet membrane-cloaked nanocarriers for the treatment of ischemic stroke. J Nanobiotechnology 20:249 (2022). PubMed: 35642036
    • Luo X  et al. Regulating Acidosis and Relieving Hypoxia by Platelet Membrane-Coated Nanoparticle for Enhancing Tumor Chemotherapy. Front Bioeng Biotechnol 10:885105 (2022). PubMed: 35646869
    • Zheng C  et al. Characterization of Novel P-Selectin Targeted Complement Inhibitors in Murine Models of Hindlimb Injury and Transplantation. Front Immunol 12:785229 (2021). PubMed: 34899752

    Customer reviews and Q&As

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