Anti-CD63 antibody (ab231975)
Key features and details
- Rabbit polyclonal to CD63
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-CD63 antibody
See all CD63 primary antibodies -
Description
Rabbit polyclonal to CD63 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human, Pig -
Immunogen
Recombinant fragment corresponding to Human CD63 aa 100-250. N-terminal His and GST tags, Expressed in E.coli.
Database link: P08962 -
Positive control
- WB: Recombinant human CD63 protein; Human serum; Pig liver lysate; A-375 cell lysate. IHC-P: Human colon cancer, stomach, prostate adenoma, skin cancer, breast cancer and liver tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.06% Proclin 300
Constituents: 44.09% PBS, 55.77% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231975 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 - 20 µg/ml.
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| WB |
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa.
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| Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
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WB
Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
Target
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Function
This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. -
Tissue specificity
Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages. -
Sequence similarities
Belongs to the tetraspanin (TM4SF) family. -
Cellular localization
Cell membrane. Lysosome membrane. Late endosome membrane. Also found in Weibel-Palade bodies of endothelial cells. Located in platelet dense granules. - Information by UniProt
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Database links
- Entrez Gene: 967 Human
- Omim: 155740 Human
- SwissProt: P08962 Human
- Unigene: 445570 Human
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Alternative names
- Lysosomal associated membrane protein 3 antibody
- CD 63 antibody
- CD63 antibody
see all
Images
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Western blot - Anti-CD63 antibody (ab231975)All lanes : Anti-CD63 antibody (ab231975) at 2 µg/ml
Lane 1 : Human serum
Lane 2 : Pig liver lysate
Lane 3 : A375 (human malignant melanoma cell line) cell lysate
Secondary
All lanes : HRP-Linked Guinea pig anti-Rabbit at 1/2000 dilution
Predicted band size: 26 kDa -
Western blot - Anti-CD63 antibody (ab231975)Anti-CD63 antibody (ab231975) at 2 µg/ml + Recombinant human CD63 protein
Predicted band size: 26 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody (ab231975)Formalin-fixed, paraffin-embedded human liver tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody (ab231975)Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody (ab231975)Formalin-fixed, paraffin-embedded human skin cancer tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody (ab231975)Formalin-fixed, paraffin-embedded human prostate adenoma tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody (ab231975)Formalin-fixed, paraffin-embedded human stomach tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody (ab231975)Formalin-fixed, paraffin-embedded human colorectal cancer tissue stained for CD63 using ab231975 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab231975 has been referenced in 5 publications.
- Lai C et al. Synovial fibroblast-miR-214-3p-derived exosomes inhibit inflammation and degeneration of cartilage tissues of osteoarthritis rats. Mol Cell Biochem 478:637-649 (2023). PubMed: 36001206
- Northrop-Albrecht EJ et al. Assessment of extracellular vesicle isolation methods from human stool supernatant. J Extracell Vesicles 11:e12208 (2022). PubMed: 35383410
- Lucien F et al. Poly (ADP-Ribose) and α-synuclein extracellular vesicles in patients with Parkinson disease: A possible biomarker of disease severity. PLoS One 17:e0264446 (2022). PubMed: 35395000
- Li B et al. Synchronous Disintegration of Ferroptosis Defense Axis via Engineered Exosome-Conjugated Magnetic Nanoparticles for Glioblastoma Therapy. Adv Sci (Weinh) 9:e2105451 (2022). PubMed: 35508804
- Hua R et al. Small RNA-seq analysis of extracellular vesicles from porcine uterine flushing fluids during peri-implantation. Gene 766:145117 (2021). PubMed: 32920039